LOCUS       sequence-478402-        7203 bp    DNA     circular SYN 14-JUL-2025
DEFINITION  CDS of HispS from N. nambi codon optimized for N.benthamiana. This 
            gene is the first step of the bioluminiscente pathway..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7203)
  AUTHORS   Calvache C, Vazquez-Vilar M, Moreno-Gimenez E, Orzaez D
  TITLE     A quantitative autonomous bioluminescence reporter system with a 
            wide dynamic range for Plant Synthetic Biology.
  JOURNAL   Plant Biotechnol J. 2024 Jan;22(1):37-47. doi: 10.1111/pbi.14146. 
            Epub 2023 Oct 26.
  PUBMED    37882352
REFERENCE   2  (bases 1 to 7203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14146";
            journalName: "Plant Biotechnol J"; date: "2024-01"; volume: "22"; 
            issue: "1"; pages: "37-47"
COMMENT     Sequence Label: GB3471
FEATURES             Location/Qualifiers
     source          1..7203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      5160..5217
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(5241..5258)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5412..6000)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5492..5511)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(6183..6277)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(6298..6957)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     6872..6891
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     7119..7137
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(7141..7184)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    join(7187..7203,1..5)
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 tagagcgatg agtggtctcg aatgaactca agtaaaaatc ctccttctac tctccttgat
       61 gttttcttgg ataccgcaag aaacttggat acagcaagca gaaatgtgct cgagtgtggt
      121 gaacatcgat ggagttacag agaactggat acagtgtcct cagctctcgc ccagcatctt
      181 cgatacacgg tggggctgtc ccctactgtc gcagtgatca gcgagaatca cccgtacata
      241 ttggccctca tgctcgctgt atggaaattg ggcggtacct tcgctccgat agatgtccac
      301 agccctgcag aactggttgc cgggatgctt aacatagtct caccgtcttg cctggtcatc
      361 ccgtcatcag acgtaacaaa ccagaccctt gcatgtgatc ttaacattcc agtggttgca
      421 tttcatcctc accaaagcac aatcccggaa ctgaacaaaa agtacctgac ggacagtcaa
      481 atcagccctg atctcccatt ccccgatccc aaccgtccgg ctctttacct gttcacttct
      541 tctgctacga gtagatccaa cttgaagtgc gtgcccctga cccacacctt catactgaga
      601 aatagcctgt caaagagggc ctggtgcaaa agaatgaggc cagaaacaga tttcgatggt
      661 ataagggttt taggatgggc accatggagt catgttctgg ctcacatgca agatataggg
      721 ccccttacac tcctgaatgc tggatgttac gtctttgcaa cgacaccgag cacatatcca
      781 accgagttga aggacgatag ggatgtcatt tcttgcgccg caaatgcagt tatgtacaag
      841 ggggttaagt ccttcgcctg tctcccgttt gtgctgggag gccttaaggc cctgtgtgag
      901 tccgagccgt ccgtcaaagc acaattacag gtcgaggagc gagcacagtt gttaaagagc
      961 ttacaacaca tgaagattct tgaatgtggt ggcgctatgc tcgaagtttc agtggcttct
     1021 tgggctatcg agaatcgtat tcccattagt ataggaatcg gtatgaccga aactggcgga
     1081 gccttgttcg ctggacccgt tcaagcaatc caaacgggat ttagttcaga ggacaagttt
     1141 attgaggatg ccacatatct ccttgtcaaa gacgattacg agtcacacgc agaagaggac
     1201 atcaatgaag gcgaactggt tgttaaaagc cgaatgctgc caaggggtta tctggggtat
     1261 aacgatccat ccttctcagt ggacgatgca gggtgggtta ccttcaaaac aggcgacagg
     1321 tactccgtga ccccggatgg taagttttct tggttaggaa ggaatacaga ctttatacag
     1381 atgacaagcg gtgagactct cgatcccagg cccatcgaaa gtttgctttg cgagtcctcc
     1441 cttatatcaa gagcctgtgt aataggcgat aagttcctga atgggcccgc cacagcagtg
     1501 tgtgccataa tagaattgga gccgactacg gtagagaaag gtcaggccca tagcagggac
     1561 atagcccgaa tttttgcccc cataaaccgt gatttgcctc ctcctttaag aatcgcctgg
     1621 agccacgtcc tcgtgctcca acccagtgaa aaaataccga tgaccaaaaa aggaacgatc
     1681 ttccgtaaga agatcgaaca agtctttggt agtgcacttg gtgggtcctc aggtgacaat
     1741 tcccaagcaa ccactgatgc atcagtcgtc cgtcgagatg aactgtcaaa cactgttaag
     1801 catatcatat cacgtgttct gggcgtgagc gacgacgagt tgctgtggac tctctcattt
     1861 gccgaacttg gaatgacctc cgcccttgca acgagaattg ccaatgaatt gaatgaggtt
     1921 ttagtagggg tcaatctgcc tatcaatgca tgttatatac acgttgactt accatccctg
     1981 tcaaatgcag tatatgccaa actggctcac ttgaaactcc ctgacagaac accagagccg
     2041 cgtaaggcac cggttgagaa tcctggcgga aaagagatcg taatagtggg acaagcattt
     2101 agacttccag gctctattaa tgacgtggct agtctccgtg acgcttttct ggcccgtcaa
     2161 gcaagttcaa tcattacaga aataccacca gacagatggg atcacgcatc cttctacccg
     2221 aaggacatac gtttcaacaa ggccggctta gtggacattg ctaactacga ccacagtttc
     2281 ttcggcttga cggctacaga agctctttac ttatctccga ccatgagact cgccctggaa
     2341 gttagttttg aggcccttga gaacgcaaac attccggttt ctcagctgaa ggggtcacaa
     2401 acagctgtct acgttgctac cacggatgac gggttcgaga ctctcttaaa tgcagaggct
     2461 gggtacgacg cttacacccg attctacggt acggggcgtg cagcttccac ggcatccggc
     2521 aggatctcat acctgcttga cgttcatgga cccagtataa ctgtggacac ggcatgtagt
     2581 ggaggggctg tttgcattga tcaagctata gactacctgc aatccagttc agccgctgat
     2641 accgccatca tttgtgcttc taatactcat tgctggccag gatcattcat gtttctgagc
     2701 gcccagggta tggtaagctc cggcggacga tgcgctactt tcacgaccga tgccgacggc
     2761 tacgtaccta gcgagggtgc agtcgccttc attcttaaaa cacgtgaagc agctatgagg
     2821 gacaaagaca caattctggc taccatcaaa gcaacacaaa tttctcataa tggtaggtcc
     2881 cagggattag tcgcccccaa tgtaaattcc caagccgact tgcatagaag cctgctccaa
     2941 aaagccgggc tttcaccggc cgacattcat ttcatcgagg cacacggaac tggaacctcc
     3001 ctcggagatc tgtccgagat ccaagctata aatgatgcat atacgagctc acaacctcga
     3061 acggccggcc cgctgatcgt ttctgcaagc aagacagtga taggacacac tgaaccagcc
     3121 ggtccgttgg tggggatgct gtcagtactg aactccttta aagagggggc cgtccctggt
     3181 ttggcacact tgaccgccga taacctgaat cccgctcttg attgcagcag tgttccgtta
     3241 ctcatacctt accagcccgt tcacctggca gcccccaagc ctcatcgtgc tgctgtaatg
     3301 tcatatggat tctctggcac cctcggtggg atcgtgctgg aggcaccaga tgaggaaaga
     3361 ctcgaagagg aacctcctaa tgacaaacct atgttgtttg tagttagtgc aaaaacacat
     3421 actgcattaa tagaatatct cggccgatac cttgaattcc tcttgcaggc aaatccacaa
     3481 gacttctgcg atatttgtta tacttcatgc gttgggcgtg aacattatcg atacagattc
     3541 gcatgcgtag ccaacgacat ggaggattta ataggacaat tgcagaagcg tttaggctct
     3601 aaggtcccac cgaaaccttc ctacaaaaga ggggcccttg cattcgcctt cagcggtcag
     3661 ggcacacagt tccgagggat ggctaccgag ctcgcaaaag cctattccgg attccgaaaa
     3721 atagtctctg atctcgctaa gagggcaagt gaactctcag gtcacgctat tgatcgattt
     3781 ttgttagctt acgatattgg ggctgaaaac gtcgccccgg atagcgaggc tgatcaaata
     3841 tgtatcttcg tatatcaatg tagcgtgctt cgttggcttc agacaatggg cattagacca
     3901 tctgctgtta tagggcactc cctcggtgag attagcgctt ctgtcgccgc cggtgcattg
     3961 tctctggact ctgcactgga ccttgttatc agtcgagctc gactgttaag gagctctacc
     4021 aatgcacccg ccggtatggc agccatgtca gcatcacagg acgaagttgt tgagttgatt
     4081 gggaaattag accttgataa agcaaattca ctctctgttt ctgttatcaa tggtcctcag
     4141 aatacggtag taagcggttc ttcagcagcc attgaaagta tagtcgcact ggccaaaggg
     4201 cgaaagataa aagcaagtgc tctcaacatc aaccaagcct tccactctcc ctacgtagat
     4261 tctgcagtac caggtttgcg tgcttggtca gagaagcata tttcctctgc tagaccatta
     4321 cagatccccc tgtactctac actccttggc gcccaagtaa gtgagggaca aatgctgaat
     4381 ccggatcatt gggtagatca cgcaagaaaa cctgtccaat tcgctcaggc cgccaccatt
     4441 atgaaagaaa gctttaccgg agtaatcatt gacataggtc ctcaagtagt tgcttggagt
     4501 ttgctgctct caaacggact gacatcagtt accgctctcg ccgcaaagcg aggacgatca
     4561 cagcaggttg ccttccttag tgctcttgca gatttatacc aagactatgg tgtcgtgcct
     4621 gactttgtgg gattatacgc tcaacaggag gacgccagtc gtttaaagaa aaccgatatt
     4681 ctgacctatc ctttccaaag ggtgagaagg tatccatctt tcatcccgag tcgtagggca
     4741 cctactcatg cccatgtcca agatgaggaa acattgtcat ctggatcttc cacacccaca
     4801 ttggagaata cagatctcga ctccggtaaa gagtctttga tgggacccac acgtggcctt
     4861 cttcgagtag acgatctgag agacagtatc gtttcttctg taaaggatgt actggagctg
     4921 aaatcaaatg aggacttgga cttgtccgag tccttgaacg cattgggaat ggacagtatt
     4981 atgttcgctc aattacgtaa aaggataggg gagggactcg ggttatccgt cccgatggta
     5041 ttcctgtctg acgccttctc aatcggcgag atggttagta accttgtaga acaggcagag
     5101 gctagtgagg acaactgagc tttgagacca ctcatcgcga ctagtagcgg ccgctgcagt
     5161 ccggcaaaaa agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta
     5221 cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     5281 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     5341 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     5401 ttgctggcgt ttttccacag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     5461 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     5521 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     5581 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     5641 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     5701 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     5761 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     5821 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     5881 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     5941 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     6001 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     6061 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     6121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ctcgaggctt
     6181 ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg
     6241 agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta
     6301 cgccccgccc tgccactcgt cgcagtactg ttgtaattca ttaagcattc tgccgacatg
     6361 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
     6421 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
     6481 gtttaaatca aaactggtga aactcaccca gggattggct gatacgaaaa acatattctc
     6541 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
     6601 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagtgatg aaaacgtttc
     6661 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
     6721 gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat
     6781 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
     6841 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
     6901 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
     6961 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat
     7021 ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aactcgagtg ccacctgacg
     7081 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga
     7141 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc
     7201 ttc
//
