LOCUS       sequence-478410-        9441 bp    DNA     circular SYN 08-AUG-2025
DEFINITION  A synthetic transcription factor (synTF) that contains a synthetic 
            general 3CLpro cleavage site (CS).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9441)
  TITLE     Optimized Pipeline and Designer Cells for Synthetic Biology Based 
            High-Throughput Screening of Viral Protease Inhibitors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 8, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSE163: pFuGW-SSX1p-GAL4BD-1X3CLpro synthetic
            ...
FEATURES             Location/Qualifiers
     source          1..9441
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     202..218
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     203..219
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    222..810
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             328..339
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     737..757
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             1675..2115
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             2278..2298
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    2386..2503
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2801..2845)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3030..3263
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3756..3881
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3928..4108
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4126..4324)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4151..4171)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4326..4705
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     4877..4896
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4971..5075
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5076..5144,5145..5936)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5294..5313)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6107..6695
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6596..6615
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6849..6866
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6983..7004
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7019..7024,7025..7042,7043..7049)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7057..7073
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7062..7084
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7081..7097
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7081..7097
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    7146..7267
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7157..7176)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7211..7230
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7397..7771)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(7790..7837)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(7885..8214)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7899..8034
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7953..7972)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8199..8219
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(8228..8656)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8338..8359)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8550..8569
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    8702..8926
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8915..8932
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             8958..9138
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 gtgaattagc ccttccagtc cccccttttc ttttaaaaag tggctaagat ctacagctgc
       61 cttgtaagtc attggtctta aaggtacctg aggtgtgact ggaaaaccca cctcctcctc
      121 ctcttgtgct tctagccagg cacaatcagc attggtagct gctgtattgc tacttgtgat
      181 tgctccatgt ttttctaggt ctcgaggtcg acggtatcga tgcggggagg cggcccaaag
      241 ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca gagccggcag
      301 caggccgcgg gaaggaaggt ccgctggatt gagggccgaa gggacgtagc agaaggacgt
      361 cccgcgcaga atccaggtgg caacacaggc gagcagccaa ggaaaggacg atgatttccc
      421 cgacaacacc acggaattgt cagtgcccaa cagccgagcc cctgtccagc agcgggcaag
      481 gcaggcggcg atgagttccg ccgtggcaat agggaggggg aaagcgaaag tcccggaaag
      541 gagctgacag gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa acacagtgca
      601 caccacgcca cgttgcctga caacgggcca caactcctca taaagagaca gcaaccagga
      661 tttatacaag gaggagaaaa tgaaagccat acgggaagca atagcatgat acaaaggcat
      721 taaagcagcg tatccacata gcgtaaaagg agcaacatag ttaagaatac cagtcaatct
      781 ttcacaaatt ttgtaatcca gaggttgatt atcgataagc ttgatatcgg cggccgccct
      841 gcagggtagc cagatcatgg ctcactgcaa cctcgtactc ctgggctcaa gctatcctcc
      901 tacctcagcc tcctgagtaa cggactacag gcacaccacc ccacctcgct aattttattt
      961 attttttttg tagagaaaag agacagggta ttgctctgtt gcccagggtg gggtgcagtg
     1021 gcatgatcat ggctcactgc aacctctgcc tcccaggttc aagtgatcct ccagctgtgg
     1081 cctccctaag tgctgggatt acaaccgtga gccgccgcac cggcccaaat ttcttacgtc
     1141 actacagagt tcctaggaaa aaatcccata cctgaaaaag atagaaactg acaggaagga
     1201 tttgagatga tgacctgctt catatacact ccttattaaa actggataac aatgcaccac
     1261 cgaggaggtg gggagggata ggaaaaatga aaagagaaaa tcagcgcatg cgtactctga
     1321 tttgggaaga ctccaaagag aaaatcagag catgcgtact ctgaacttgg agtagccaat
     1381 cccaggggat gctttaggcg ggaaagtcag agtttctgcc tccattttga gaaggttctg
     1441 tccctagagc ctagactgat agaccccaca tcagcttggc ttgtcccgcc tactgttctg
     1501 acttctgatt ggccagatgg agttcactaa ctgccctgat tggtccatca tcctggagca
     1561 atgacattgc agaatatttt ctcctcctcc agccacactt tgtcaccaac tgctgccaac
     1621 tcgccaccac tgctgccgac ctcgcaacca ctgctttgtc tctggcgcgc catgatgaag
     1681 ctactgtctt ctatcgaaca agcatgcgat atttgccgac ttaaaaagct caagtgctcc
     1741 aaagaaaaac cgaagtgcgc caagtgtctg aagaacaact gggagtgtcg ctactctccc
     1801 aaaaccaaaa ggtctccgct gactagggca catctgacag aagtggaatc aaggctagaa
     1861 agactggaac agctatttct actgattttt cctcgagaag accttgacat gattttgaaa
     1921 atggattctt tacaggatat aaaagcattg ttaacaggat tatttgtaca agataatgtg
     1981 aataaagatg ccgtcacaga tagattggct tcagtggaga ctgatatgcc tctaacattg
     2041 agacagcata gaataagtgc gacatcatca tcggaagaga gtagtaacaa aggtcaaaga
     2101 cagttgactg tatcggtagc ccgcttgcaa agcgggttct cgacggcccc cccgaccgat
     2161 gtcagcctgg gggacgagct ccacttagac ggcgaggacg tggcgatggc gcatgccgac
     2221 gcgctagacg atttcgatct ggacatgttg ggggacgggg attccccggg tccgggacca
     2281 aagaagaagc ggaaggtcta gggatccttg cagtcccggg taagcgggac tctggggttc
     2341 gaaaattaac caaactggat ctctgctgtc cctgtaataa acccgaaaat tttgaatttt
     2401 tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt gctattatgt ctactattct
     2461 ttcccctgca ctgtaccccc caatcccccc ttttctttta aaattgtgga tgaatactgc
     2521 catttgtctg cagaattggc gcacgcagtg ccgatccgtt cactaatcga atggatctgt
     2581 ctctgtctct ctctccacct tcttcttcta ttccttcggg cctgtcgggt cccctcgggg
     2641 ttgggaggtg ggtctgaaac gataatggtg aatatccctg cctaactcta ttcactatag
     2701 aaagtacagc aaaaactatt cttaaaccta ccaagcctcc tactatcatt atgaataatt
     2761 ttatatacca cagccaattt gttatgttaa accaattcca caaacttgcc catttatcta
     2821 attccaataa ttcttgttca ttcttttctt gctggttttg cgattcttca attaaggagt
     2881 gtattaagct tgtgtaattg ttaatttctc tgtcccactc catccaggtc gtgtgattcc
     2941 aaatctgttc cagagattta ttactccaac tagcattcca aggcacagca gtggtgcaaa
     3001 tgagttttcc agagcaaccc caaatcccca ggagctgttg atcctttagg tatctttcca
     3061 cagccaggat tcttgcctgg agctgcttga tgccccagac tgtgagttgc aacagatgct
     3121 gttgcgcctc aatagccctc agcaaattgt tctgctgctg cactatacca gacaataatt
     3181 gtctggcctg taccgtcagc gtcattgacg ctgcgcccat agtgcttcct gctgctccca
     3241 agaacccaag gaacaaagct cctattccca ctgctctttt ttctctctgc accactcttc
     3301 tctttgcctt ggtgggtgct actcctaatg gttcaatttt tactacttta tatttatata
     3361 attcacttct ccaattgtcc ctcatatctc ctcctccagg tctgaagatc agcggccgct
     3421 tgctgtgcgg tggtcttact tttgttttgc tcttcctcta tcttgtctaa agcttccttg
     3481 gtgtctttta tctctatcct ttgatgcaca caatagaggg ttgctactgt attatataat
     3541 gatctaagtt cttctgatcc tgtctgaagg gatggttgta gctgtcccag tatttgtcta
     3601 cagccttctg atgtttctaa caggccagga ttaactgcga atcgttctag ctccctgctt
     3661 gcccatacta tatgttttaa tttatatttt ttctttcccc ctggccttaa ccgaattttt
     3721 tcccatcgcg atctaattct cccccgctta atactgacgc tctcgcaccc atctctctcc
     3781 ttctagcctc cgctagtcaa aatttttggc gtactcacca gtcgccgccc ctcgcctctt
     3841 gccgtgcgcg cttcagcaag ccgagtcctg cgtcgagaga gctcctctgg tttccctttc
     3901 gctttcaagt ccctgttcgg gcgccactgc tagagatttt ccacactgac taaaagggtc
     3961 tgagggatct ctagttacca gagtcacaca acagacgggc acacactact tgaagcactc
     4021 aaggcaagct ttattgaggc ttaagcagtg ggttccctag ttagccagag agctcccagg
     4081 ctcagatctg gtctaaccag agagacccag tacaggcaaa acgcgctgct tatatagacc
     4141 tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt
     4201 ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga
     4261 cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc
     4321 atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat
     4381 aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg
     4441 ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac
     4501 tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt
     4561 attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt
     4621 tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat
     4681 taataactag tcaataatca atgtcaacgc gtatatctgg cccgtacatc gcgaagcagc
     4741 gcaaaacgcc taaccctaag cagattcttc atgcaattgt cggtcaagcc ttgccttgtt
     4801 gtagcttaaa ttttgctcgc gcactactca gcgacctcca acacacaagc agggagcaga
     4861 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca taggggatcg
     4921 ggagatctcc cgatccgtcg acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     4981 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     5041 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     5101 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     5161 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     5221 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     5281 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     5341 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     5401 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5461 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     5521 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5581 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     5641 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     5701 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     5761 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     5821 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     5881 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     5941 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     6001 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     6061 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6121 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6181 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     6241 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6301 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6361 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6421 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6481 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6541 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6601 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6661 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6721 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     6781 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     6841 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     6901 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     6961 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     7021 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     7081 caggaaacag ctatgaccat gattacgcca agctctagct agaggtcgac ggtatacaga
     7141 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     7201 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     7261 acaagttggg gtgggcgaag aactccagca tgagatcccc gcgctggagg atcatccagc
     7321 cggcgtcccg gaaaacgatt ccgaagccca acctttcata gaaggcggcg gtggaatcga
     7381 aatctcgtag cacgtgtcag tcctgctcct cggccacgaa gtgcacgcag ttgccggccg
     7441 ggtcgcgcag ggcgaactcc cgcccccacg gctgctcgcc gatctcggtc atggccggcc
     7501 cggaggcgtc ccggaagttc gtggacacga cctccgacca ctcggcgtac agctcgtcca
     7561 ggccgcgcac ccacacccag gccagggtgt tgtccggcac cacctggtcc tggaccgcgc
     7621 tgatgaacag ggtcacgtcg tcccggacca caccggcgaa gtcgtcctcc acgaagtccc
     7681 gggagaaccc gagccggtcg gtccagaact cgaccgctcc ggcgacgtcg cgcgcggtga
     7741 gcaccggaac ggcactggtc aacttggcca tggtttagtt cctcaccttg tcgtattata
     7801 ctatgccgat atactatgcc gatgattaat tgtcaacacg tgctgatcag atccgaaaat
     7861 ggatatacaa gctcccggga gctttttgca aaagcctagg cctccaaaaa agcctcctca
     7921 ctacttctgg aatagctcag aggcagaggc ggcctcggcc tctgcataaa taaaaaaaat
     7981 tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg
     8041 agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca tacttctgcc
     8101 tgctggggag cctggggact ttccacacct ggttgctgac taattgagat gcatgctttg
     8161 catacttctg cctgctgggg agcctgggga ctttccacac cctaactgac acacattcca
     8221 cagaattaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     8281 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     8341 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     8401 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     8461 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     8521 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     8581 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     8641 ccgctacagg gcgcgtgggg atacccccta gagccccagc tggttctttc cgcctcagaa
     8701 gccatagagc ccaccgcatc cccagcatgc ctgctattgt cttcccaatc ctcccccttg
     8761 ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca atgcgatgca
     8821 atttcctcat tttattagga aaggacagtg ggagtggcac cttccagggt caaggaaggc
     8881 acgggggagg ggcaaacaac agatggctgg caactagaag gcacagtcga ggctgatcag
     8941 cgggtttaaa cgggccctgc tagagatttt ccacactgac taaaagggtc tgagggatct
     9001 ctagttacca gagtcacaca acagacgggc acacactact tgaagcactc aaggcaagct
     9061 ttattgaggc ttaagcagtg ggttccctag ttagccagag agctcccagg ctcagatctg
     9121 gtctaaccag agagacccag tacagtccgg atgcagctct cgggccatgt gatgaaatgc
     9181 taggcggctg tcaaacctcc actctaatac ttctctctcc gggtcatcca tcccatgcag
     9241 gctcacaggg tgtaacaagc gggtgttctc tccttcattg gcttcttcta ccttctcttg
     9301 ctcaactggt actagcttgt agcaccatcc aaaggtcagt ggatatctga tccctggccc
     9361 tggtgtgtag ttctgccaat cagggaagta gccttgtgtg tggtagatcc acagatcaag
     9421 gatatcttgt cttcgttggg a
//
