LOCUS       sequence-478434-       12109 bp    DNA     circular SYN 17-JUL-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12109)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 12109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-NOTCH2(Trunc1)-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..12109
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             7759..7782
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             9442..9486
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             9487..9513
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(9653..9670)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9659..9770
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9888..9908)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9893..10222
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10073..10208
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10135..10154
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10270..10317
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10336..10935
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10336..10355)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10832..10852
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    11065..11186
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11102..11121)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11156..11175
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(11239..12030,12031..12099))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11862..11881
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(12100..12109,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgcctt gcagtgtaga
     2761 gatggctacg agccctgcgt gaacgaaggg atgtgcgtga cctatcacaa cggcaccggc
     2821 tactgcaagt gccctgaggg ctttctgggc gagtactgcc agcacagaga tccctgcgag
     2881 aagaacagat gccagaacgg cggaacatgt gtggcccagg ctatgctggg caaagccaca
     2941 tgtagatgcg ccagcggctt taccggcgag gattgccagt acagcaccag ccatccttgc
     3001 ttcgtgtcca gaccttgcct gaatggcggc acctgtcaca tgctgagcag agacacctac
     3061 gagtgtacct gccaagtggg cttcaccggc aaagaatgcc agtggaccga tgcctgcctg
     3121 tctcaccctt gtgccaatgg cagcacatgc accaccgtgg ccaatcagtt cagctgcaag
     3181 tgtctgaccg gcttcacagg ccagaaatgc gagacagacg tgaacgagtg cgacatccct
     3241 ggacactgtc agcatggcgg aacctgcctg aatctgcctg gcagctatca gtgtcagtgc
     3301 ccacagggat tcaccggcca gtactgtgat agcctgtacg tgccctgcgc tccctctcct
     3361 tgtgttaacg gtggcacctg tagacagacc ggcgacttca ccttcgagtg caattgcctg
     3421 ccaggcttcg agggctctac ctgcgagaga aacatcgacg actgccccaa ccacaggtgt
     3481 caaaatggcg gagtgtgtgt ggacggcgtg aacacctaca actgcagatg tcctcctcag
     3541 tggacaggcc agttctgcac cgaggatgtg gatgagtgcc tgctgcagcc taacgcttgt
     3601 cagaatggtg gcacatgcgc caacagaaat ggcggctatg gctgcgtgtg tgtgaatgga
     3661 tggtccggcg acgactgtag cgagaacatc gatgattgcg ccttcgccag ctgtacccct
     3721 ggcagcacct gtatcgatag agtggccagc ttctcctgca tgtgcccaga aggcaaagcc
     3781 ggactgctgt gtcatctgga tgacgcctgc atcagcaacc catgtcacaa aggcgccctg
     3841 tgcgacacca atcctctgaa tggccagtac atctgcacct gtcctcaggg ctataagggc
     3901 gccgactgta cagaggacgt ggacgaatgt gccatggcca acagcaatcc ttgcgagcac
     3961 gccggcaaat gtgtgaacac agatggcgcc ttccactgcg agtgcctgaa aggatatgcc
     4021 ggacctagat gcgagatgga catcaacgag tgccacagcg acccctgcca gaatgatgcc
     4081 acctgtctgg acaagatcgg cggcttcact tgcctgtgca tgcctggctt taagggcgtg
     4141 cactgcgagc tggaaatcaa tgaatgtcaa agcaacccct gcgtcaacaa cggccagtgc
     4201 gtggacaaag tgaacagatt ccagtgcctg tgtcctcctg gcttcactgg cccagtgtgc
     4261 cagatcgaca ttgacgattg cagcagcacc ccttgtctga acggcgccaa gtgcatcgat
     4321 caccctaacg gctatgagtg ccagtgcgct acaggcttta caggcgtgct gtgcgaagag
     4381 aatatcgaca actgcgaccc cgatccttgc caccacggac agtgtcagga cggcatcgac
     4441 agctacacat gcatctgcaa tcctggctac atgggagcca tctgcagcga ccagatcgat
     4501 gagtgctaca gcagcccatg cctgaacgac ggcagatgta tcgacctggt caacggctac
     4561 cagtgcaact gtcagcctgg aaccagcgga gtgaactgcg agatcaactt cgacgattgt
     4621 gccagcaatc cctgcatcca cggcatctgc atggatggca tcaacagata cagctgtgtg
     4681 tgcagccccg ggtttaccgg ccagagatgc aacattgata tcgacgagtg cgcctccaat
     4741 ccatgccgga agggcgctac atgcatcaat ggcgtgaacg gcttccggtg tatctgccct
     4801 gaaggacctc accatcctag ctgctacagc caagtgaacg aatgcctgag caacccttgt
     4861 atccacggaa actgtacagg cggcctgagc ggctacaaat gtctgtgtga tgccggctgg
     4921 gtcggaatca actgcgaggt ggacaagaat gagtgtctgt ctaatccctg ccaaaacggt
     4981 ggaacttgcg acaacctcgt gaatggctac agatgtacct gcaagaaggg attcaagggg
     5041 tacaactgcc aagtcaacat agatgagtgc gcttctaacc cctgtctcaa ccagggcacc
     5101 tgtttcgacg acatcagcgg ctatacctgc cactgcgtgc tgccttacac cggcaagaac
     5161 tgtcagacag tgctggcccc ttgcagccct aatccttgtg aaaacgccgc cgtgtgcaaa
     5221 gagagcccca acttcgagtc ttacacatgc ctgtgcgccc ctggatggca gggacagaga
     5281 tgtaccatcg atattgatga gtgtatcagc aagccttgca tgaatcacgg cctgtgccac
     5341 aacacccagg gctcctatat gtgcgagtgt ccaccaggct ttagcggcat ggactgcgag
     5401 gaagatattg acgactgtct ggctaaccca tgccagaatg gcggctcttg tatggacggg
     5461 gtcaacactt ttagctgtct gtgtctgcct gggttcaccg gggataagtg ccagaccgat
     5521 atgaatgaat gcctcagcga gccctgtaaa aacggcggga cctgcagcga ttacgtgaac
     5581 tcttacacct gtaaatgcca ggccggcttc gacggggtgc actgtgaaaa caatatcaat
     5641 gagtgcaccg agtccagctg cttcaacggc ggcacttgcg tggacggcat taacagcttt
     5701 tcttgtctgt gccccgtggg ctttactggc agcttctgtc tgcacgagat taacgagtgc
     5761 agctctcacc catgtctgaa tgagggaacc tgtgtggatg gcctgggcac ctacagatgc
     5821 tcttgtcctc tgggatacac agggaagaat tgccagacac tggtcaacct gtgtagcaga
     5881 agcccttgca agaacaaggg cacatgtgtg cagaagaaag ccgagagcca gtgtctctgt
     5941 ccttctggat gggctggcgc ctactgtgat gtgcccaatg tgtcttgtga tatcgccgcc
     6001 agtagacggg gagtgctggt ggaacatctg tgtcagcaca gcggcgtgtg catcaacgcc
     6061 ggaaataccc actactgcca gtgtccactg ggctataccg gctcctattg cgaggaacag
     6121 ctggacgagt gtgcaagtaa cccttgtcag cacggggcca cctgttctga cttcatcgga
     6181 ggatacagat gcgagtgcgt gccaggatat cagggcgtga actgtgaata cgaagttgac
     6241 gagtgtcaga accagccttg ccaaaatggc gggacatgca tcgacctcgt gaaccacttc
     6301 aagtgcagct gccctcctgg cacaagaggc ctgctctgtg aagaaaacat tgatgactgt
     6361 gccagaggac cccactgtct caatggcggc cagtgtatgg atcggattgg cggctactcc
     6421 tgtagatgtc tgccaggatt tgccggggag agatgcgagg gcgacattaa cgaatgtctc
     6481 agtaacccct gcagcagcga gggcagcctg gattgcatcc agctgaccaa cgactacctg
     6541 tgcgtgtgca gatccgcctt taccggaagg cactgcgaaa ccttcgtgga cgtgtgccct
     6601 cagatgcctt gtcttaatgg cggaacttgc gccgtggcca gcaacatgcc tgatggcttt
     6661 atctgtcgct gcccacctgg attttccggc gctagatgtc aatcctcctg cggacaagtg
     6721 aagtgcagaa agggcgagca gtgcgtgcac acagccagcg gaccaagatg cttctgccca
     6781 tctcctagag attgcgagag cggctgtgct tctagtccct gtcaacacgg cggaagctgt
     6841 caccctcaga gacagccacc ttactacagc tgtcagtgcg ccccaccttt tagcggctcc
     6901 agatgcgaac tgtacacagc ccctccaagc acacctcctg ccacatgtct gtcccagtat
     6961 tgcgccgaca aggcaagaga tggcgtgtgc gacgaggcct gtaattctca cgcatgtcaa
     7021 tgggatggcg gcgactgcag cctgaccatg gaaaatcctt gggccaactg cagctcccct
     7081 ctgccttgct gggactacat caacaaccag tgcgacgaac tgtgcaacac cgtggaatgc
     7141 ctgttcgaca atttcgagtg ccagggcaac tccaagacct gcaaatacga caagtactgc
     7201 gccgatcact tcaaggacaa ccactgcgat cagggctgta acagcgagga atgtggctgg
     7261 gatggactgg attgtgctgc cgaccagcct gaaaatctgg ccgagggaac actcgtgatc
     7321 gtggtgctga tgcctcctga acagctgctc caggacgcca gatcttttct gagagcactg
     7381 ggcaccctgc tgcacaccaa cctgagaatc aagagagact cccagggcga gctgatggtg
     7441 tacccctact atggcgagaa gtccgccgcc atgaagaaac agcggatgac cagaagatct
     7501 ctgcccggcg agcaagagca agaggtggcc ggaagcaagg tgttcctgga aatcgacaac
     7561 cggcagtgtg tccaggactc cgaccactgc ttcaagaaca ctgatgccgc tgccgctctg
     7621 ctggcctctc atgctattca gggcacactg agctaccctc tggtgtctgt ggtgtccgag
     7681 agcctgacac ctgagagaac ccaaggcgcg ccggaagatc aagttgatcc aagattgatt
     7741 gatggcaagg gcggatctct ggaggtgctg ttccagggac caggcggaag cggcaatccc
     7801 ggcggcgatc tggcccccca gatgctgcgc gagctgcagg agaccaatgc cgccctgcag
     7861 gacgtgcgcg agctgctgcg ccaccgcgtg aaggagatca ccttcctgaa gaacaccgtg
     7921 atggagtgcg acgcctgcgg tatgcagccc tctagaggct ccggctccat catcccagtt
     7981 gaggaggaga acccggactt ctggaaccgc gaggcagccg aggccctggg tgccgccaag
     8041 aagctgcagc ctgcacagac agccgccaag aacctcatca tcttcctggg cgatgggatg
     8101 ggggtgtcta cggtgacagc tgccaggatc ctaaaagggc agaagaagga caaactgggg
     8161 cctgagatac ccctggccat ggaccgcttc ccatatgtgg ctctgtccaa gacatacaat
     8221 gtagacaaac atgtgccaga cagtggagcc acagccacgg cctacctgtg cggggtcaag
     8281 ggcaacttcc agaccattgg cttgagtgca gccgcccgct ttaaccagtg caacacgaca
     8341 cgcggcaacg aggtcatctc cgtgatgaat cgggccaaga aagcagggaa gtcagtggga
     8401 gtggtaacca ccacacgagt gcagcacgcc tcgccagccg gcacctacgc ccacacggtg
     8461 aaccgcaact ggtactcgga cgccgacgtg cctgcctcgg cccgccagga ggggtgccag
     8521 gacatcgcta cgcagctcat ctccaacatg gacattgacg tgatcctagg tggaggccga
     8581 aagtacatgt ttcgcatggg aaccccagac cctgagtacc cagatgacta cagccaaggt
     8641 gggaccaggc tggacgggaa gaatctggtg caggaatggc tggcgaagcg ccagggtgcc
     8701 cggtatgtgt ggaaccgcac tgagctcatg caggcttccc tggacccgtc tgtgacccat
     8761 ctcatgggtc tctttgagcc tggagacatg aaatacgaga tccaccgaga ctccacactg
     8821 gacccctccc tgatggagat gacagaggct gccctgcgcc tgctgagcag gaacccccgc
     8881 ggcttcttcc tcttcgtgga gggtggtcgc atcgaccatg gtcatcatga aagcagggct
     8941 taccgggcac tgactgagac gatcatgttc gacgacgcca ttgagagggc gggccagctc
     9001 accagcgagg aggacacgct gagcctcgtc actgccgacc actcccacgt cttctccttc
     9061 ggaggctacc ccctgcgagg gagctccatc ttcgggctgg cccctggcaa ggcccgggac
     9121 aggaaggcct acacggtcct cctatacgga aacggtccag gctatgtgct caaggacggc
     9181 gcccggccgg atgttaccga gagcgagagc gggagccccg agtatcggca gcagtcagca
     9241 gtgcccctgg acgaagagac ccacgcaggc gaggacgtgg cggtgttcgc gcgcggcccg
     9301 caggcgcacc tggttcacgg cgtgcaggag cagaccttca tagcgcacgt catggccttc
     9361 gccgcctgcc tggagcccta caccgcctgc gacctggcgc cccccgccgg caccaccgac
     9421 gccgcgcacc cgggtggcgg aggactgaac gacatcttcg aggctcagaa aatcgaatgg
     9481 cacgaacatc accatcacca tcaccatcac cattaaggcc ggcctgtagt cactggcaca
     9541 acgcgttacc cggtaagcca atcgggtata cacggtcgtc atactgcaga cagggttctt
     9601 ctactttgca agatagtctt gagtagtaaa ataaatagat agagaaaatc agcctcgact
     9661 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     9721 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc acaacactca
     9781 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     9841 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     9901 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     9961 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
    10021 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
    10081 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
    10141 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
    10201 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
    10261 tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
    10321 gtgaggaact aaaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
    10381 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
    10441 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
    10501 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
    10561 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
    10621 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
    10681 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
    10741 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
    10801 gtgcccgcct tcctggagac atccgcgccc cgcaacctcc ccttctacga gcggctcggc
    10861 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
    10921 aagcccggtg cctgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
    10981 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
    11041 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
    11101 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
    11161 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
    11221 ttggcgtaat catggtcatt accaatgctt aatcagtgag gcacctatct cagcgatctg
    11281 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    11341 gggcttacca tctggcccca gcgctgcgat gataccgcga gaaccacgct caccggctcc
    11401 ggatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    11461 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    11521 agttaatagt ttgcgcaacg ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc
    11581 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    11641 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    11701 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    11761 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    11821 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    11881 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    11941 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    12001 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    12061 aaagggaata agggcgacac ggaaatgttg aatactcata ttcttcctt
//
