LOCUS       sequence-478446-        4564 bp    DNA     circular SYN 14-JUL-2025
DEFINITION  Expression of MFSD6 loop 3 tagged with rabbit IgG Fc2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Varanese L, Xu L, Peters CE, Pintilie G, Roberts DS, Raj S, Liu M, 
            Ooi YS, Diep J, Qiao W, Richards CM, Callaway J, Bertozzi CR, Jabs 
            S, de Vries E, van Kuppeveld FJM, Nagamine CM, Chiu W, Carette JE
  TITLE     MFSD6 is an entry receptor for enterovirus D68.
  JOURNAL   Nature. 2025 Mar 25. doi: 10.1038/s41586-025-08908-0.
  PUBMED    40132641
REFERENCE   2  (bases 1 to 4564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2025 Mar 25. doi: 10.1038/s41586-025-08908-0."
COMMENT     Sequence Label: pFUSE-rIgG-Fc2-MFSD6(L3)-Zeo
FEATURES             Location/Qualifiers
     source          1..4564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        669..972
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             1565..1939
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1953..2347
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     polyA_signal    2451..2572
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2488..2507)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2542..2561
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     sig_peptide     complement(3669..3728)
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     LTR             3778..4046
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(4059..4270)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    4307..4398
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(4339..4358)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    4409..4457
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tcttaattaa
      661 cctgcaggcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      721 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      781 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      841 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      901 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      961 gctattacca tgatgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     1021 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttgactagtc
     1081 agggccccaa cccccccaag cccccatttc acaacacgct ggcgctacag gcgcgtgact
     1141 tccccttgct ttggggcggg gggctgagac tcctatgtgc tccggattgg tcaggcacgg
     1201 ccttcggccc cgcctcctgc caccgcagat tggccgctag gcctccccga gcgccctgcc
     1261 tccgagggcc ggcgcaccat aaaagaagcc gccctagcca cgtcccctcg cagttcggcg
     1321 gtcccgcggg tctgtctcaa gcttctgcct tctccctcct gtgagtttgg taagtcactg
     1381 actgtctatg cctgggaaag ggtgggcagg agatggggca gtgcaggaaa agtggcacta
     1441 tgaaccctgc agccctagtt tgacaattaa tcattggcat agtatatctg catagtataa
     1501 tacaactcac tatagcaatt gtactaacct tcttctcttt cctctcctga caggaggagc
     1561 catcatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc
     1621 ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc
     1681 cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc
     1741 ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc
     1801 ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga
     1861 gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcactttgt
     1921 ggcagaggag caggactgag gataagctag aagctcgctt tcttgctgtc caatttctat
     1981 taaaggttcc tttgttccct aagtccaact actaaactgg gggatattat gaagggcctt
     2041 gagcatctgg attctgccta ataaaaaaca tttattttca ttgcaatgat gtatttaaat
     2101 tatttctgaa tattttacta aaaagggaat gtgggaggtc agtgcattta aaacataaag
     2161 aaatgaagag ctagttcaaa ccttgggaaa atacactata tcttaaactc catgaaagaa
     2221 ggtgaggctg caaacagcta atgcacattg gcaacagccc ctgatgccta tgccttattc
     2281 atccctcaga aaaggattca agtagaggct tgatttggag gttaaagttt tgctatgctg
     2341 tattttagaa ttaattccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     2401 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     2461 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     2521 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     2581 ggccagctag ctcatttacc cggagagcgg gagatggact tctgcgtgta gtggttgtgc
     2641 aaggcctcgt gcatcacgga gcaggtgaag acgtcgcccc gctgccactc actcgtgggc
     2701 actgagagct tgctgtagag gaagtaggag ccgtcgctgt ccagcacggc cggcgtggtc
     2761 ttgtagttgt cctctgcctt cccgttcttc tcccactcca ccgagatgtc ggaagggtag
     2821 aagccgttga tcatgcaggt caggctgacc gacctgctgc tcagctcctc ccggggaggg
     2881 cccatggtgt agaccttcgg ctccaggggc tgccctctgg ctttggagat ggttttctcg
     2941 atgggggccg ggagtgcctt gttgtggact ttgcacttga actccttgcc cctcagccag
     3001 tcctggtgcg cgatggggag ggtgctgacc acgcggatcg tgctgttgaa ctgctgctcc
     3061 cgtagcggcg gccgggcggt gcgcacctgc tcgttgttta tgtaccatgt gaactgcacc
     3121 tcggggtcat cctggctcac gtccaccacc acgcatgtga cctcgggggt gcgtgagatc
     3181 atgagggtgt ccttgggttt tggggggaag atgaagacag acggtccccc caggagttca
     3241 gggggtgggc acgtgggctt gctagatctt tcaacttctt gttgatcata aacaagcatg
     3301 acttggtcag aaggtaaaga tttggtggtg gtaacaataa cagtggttgt ctccttggtt
     3361 acgcttcctg gggagacagg ggttgctgtg cttgagttca aagtcaagtc catcatacgg
     3421 ttagtaattt cacctgtctg gggctgcaga gtgggttcac tgttcatgtt tggagctgtt
     3481 ggcaaagtaa cggtgtctga gacattgagc cttgtttcca aaaggtttct tttctcacgc
     3541 atttttggtg atatggtgag gaaagaggta aaggaagaat ttgttggcag gatagttaac
     3601 tggtgacttg cattggtggg gtgagttgtt gggcgaatct ttggtacaca tctcaaggta
     3661 gcaggtttcg aattcgtgac aagtgcaaga cttagtgcaa tgcaagacag gagttgcatc
     3721 ctgtacatgg tggccctcct tcgccggtga tctcaggtag gcgccggtca cagcttggat
     3781 ctgtaacggc gcagaacaga aaacgaaaca aagacgtaga gttgagcaag cagggtcagg
     3841 caaagcgtgg agagccggct gagtctaggt aggctccaag ggagcgccgg acaaaggccc
     3901 ggtctcgacc tgagctttaa acttacctag acggcggacg cagttcagga ggcaccacag
     3961 gcgggaggcg gcagaacgcg actcaaccgg cgtggatggc ggcctcaggt agggcggcgg
     4021 gcgcgtgaag gagagatgcg agcccctcga agcttcagct gtgttctggc ggcaaacccg
     4081 ttgcgaaaaa gaacgttcac ggcgactact gcacttatat acggttctcc cccaccctcg
     4141 ggaaaaaggc ggagccagta cacgacatca ctttcccagt ttaccccgcg ccaccttctc
     4201 taggcacccg ttcaattgcc gacccctccc cccaacttct cggggactgt gggcgatgtg
     4261 cgctctgccc actgacgggc accggagcga tcgcagatcc ttcgatagag aaatgttctg
     4321 gcacctgcac ttgcactggg gacagcctat tttgctagtt tgttttgttt cgttttgttt
     4381 tgatggagag cgtatgttag ttacgattca cacaaaaaac caacacacag atgtaatgaa
     4441 aataaagata ttttattgcg gccgctgatt taaatgttaa ttaactagcc atgaccaaaa
     4501 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     4561 cttc
//
