LOCUS       sequence-478484-        5024 bp    DNA     circular SYN 07-AUG-2025
DEFINITION  Express HiBiT-TREM2 Fusion Protein in Mammalian Cells under a CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Promega
  TITLE     Promega Plasmid Collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: HiBiT-TREM2 Vector
FEATURES             Location/Qualifiers
     source          1..5024
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1033..1052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1967..1985)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    2022..2143
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2033..2052)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2087..2106
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        2388..2745
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2388..2579
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2411..2431)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2596..2731
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        2760..2807
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2839..3633
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2893..2912)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3503..3522
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3697..3745
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(3984..4572)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4064..4083)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    4688..4971
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgct caccatgaac tccttctcca
     1081 caagcgcctt cggtccagtt gccttctccc tgggcctgct cctggtgttg cctgctgcct
     1141 tccctgcccc agtgagcggc tggcggctgt tcaagaagat tagcgggagc tccggtggct
     1201 cgagcggtgc gatcgcccac aacaccacag tgttccaggg cgtggcgggc cagtccctgc
     1261 aggtgtcttg cccctatgac tccatgaagc actgggggag gcgcaaggcc tggtgccgcc
     1321 agctgggaga gaagggccca tgccagcgtg tggtcagcac gcacaacttg tggctgctgt
     1381 ccttcctgag gaggtggaat gggagcacag ccatcacaga cgataccctg ggtggcactc
     1441 tcaccattac gctgcggaat ctacaacccc atgatgcggg tctctaccag tgccagagcc
     1501 tccatggcag tgaggctgac accctcagga aggtcctggt ggaggtgctg gcagaccccc
     1561 tggatcaccg ggatgctgga gatctctggt tccccgggga gtctgagagc ttcgaggatg
     1621 cccatgtgga gcacagcatc tccaggagcc tcttggaagg agaaatcccc ttcccaccca
     1681 cttccatcct tctcctcctg gcctgcatct ttctcatcaa gattctagca gccagcgccc
     1741 tctgggctgc agcctggcat ggacagaagc cagggacaca tccacccagt gaactggact
     1801 gtggccatga cccagggtat cagctccaaa ctctgccagg gctgagagac acggtttaaa
     1861 cgaattcggg ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat gcaagctgat
     1921 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
     1981 ctagcataac cccttggggc ggccgcttcg agcagacatg ataagataca ttgatgagtt
     2041 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     2101 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     2161 tcattttatg tttcaggttc agggggagat gtgggaggtt tttttaagca agtaaaacct
     2221 ctacaaatgt ggtaaaatcg aattttaaca aaatattaac gcttacaatt tcctgatgcg
     2281 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgcggat ctgcgcagca
     2341 ccatggcctg aaataacctc tgaaagagga acttggttag gtaccttctg aggcggaaag
     2401 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     2461 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     2521 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     2581 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     2641 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     2701 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct taattaactg
     2761 ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact
     2821 aaacccagga ggcagatcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     2881 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     2941 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     3001 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     3061 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     3121 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     3181 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     3241 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     3301 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     3361 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     3421 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     3481 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     3541 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     3601 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     3661 accaagcgac gcccaacctg ccatcacgat ggccgcaata aaatatcttt attttcatta
     3721 catctgtgtg ttggtttttt gtgtgaatcg atagcgataa ggatcctctt tgcgcttgcg
     3781 ttttcccttg tccagatagc ccagtagctg acattcatcc ggggtcagca ccgtttctgc
     3841 ggactggctt tctacccggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     3901 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     3961 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     4021 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     4081 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     4141 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     4201 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4261 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     4321 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     4381 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     4441 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     4501 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     4561 aaaggatttc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     4621 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     4681 ttatagtccg gaaatacagg aacgcacgct ggatggccct tcgctgggat ggtgaaacca
     4741 tgaaaaatgg cagcttcagt ggattaagtg ggggtaatgt ggcctgtacc ctctggttgc
     4801 ataggtattc atacggttaa aatttatcag gcgcgattgc ggcagttttt cgggtggttt
     4861 gttgccattt ttacctgtct gctgccgtga tcgcgctgaa cgcgttttag cggtgcgtac
     4921 aattaaggga ttatggtaaa tccacttact gtctgccctc gtagccatcg agataaaccg
     4981 cagtactccg gccacgatgc gtccggcgta gaggatcgag atct
//
