LOCUS       sequence-478519-        2240 bp    DNA     linear   UNA 14-JUL-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: ADORA3-Tango
FEATURES             Location/Qualifiers
     source          1..2240
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1234..1848,1849..2235)
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="RLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKN
                     KRALLDALXIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPT
                     EKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDS
                     MPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTIEG
                     LLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHADA
                     LDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(1301..1320)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 cttggtaccg agctcggatc cactagtcca gtgtggtgga attctgcaga tatccagcac
       61 agtggcggcc gcgccaccat gaagacgatc atcgccctga gctacatctt ctgcctggta
      121 ttcgccgact acaaggacga tgatgacgcc agcatcgata tgccaaacaa ctctaccgcc
      181 ttgtctctgg ctaacgttac atacatcact atggagatat tcattggtct gtgcgctatt
      241 gtggggaatg tcctggttat atgtgtggtc aagttgaatc caagtctcca gactacaaca
      301 ttttatttta ttgtgtcact ggcactggcc gacattgctg tgggtgtgtt ggtaatgcct
      361 ctggccatag tggtcagtct cgggatcaca atccattttt actcctgctt gttcatgact
      421 tgtctgctgc tgatcttcac gcacgccagc attatgagcc ttctggctat cgctgttgat
      481 cggtacctgc gcgtcaagtt gactgtgcgg tataagcggg ttaccaccca ccgcagaata
      541 tggctggctc tgggcctgtg ttggcttgtt agcttcttgg tgggcctgac ccctatgttt
      601 gggtggaaca tgaagctgac atctgaatac caccggaacg ttactttcct gagttgtcag
      661 tttgtatccg tgatgcgaat ggattatatg gtttacttca gctttctgac ttggatattt
      721 atcccgctgg tggtcatgtg tgctatatac ctggatatct tttatatcat aaggaacaag
      781 ctcagtctca atctgtcaaa cagcaaggaa actggtgcct tttatgggcg cgaattcaag
      841 actgctaaga gcctcttcct ggtgctcttt cttttcgctc tgagttggct gccactgtcc
      901 attatcaact gtatcattta ttttaacgga gaggtgccgc agctggtact ctacatggga
      961 atacttttga gccacgcaaa tagtatgatg aatcccatag tgtatgctta caagattaaa
     1021 aagtttaagg aaacctacct gctgattctg aaagcctgtg tcgtatgcca cccatctgat
     1081 agcctggata cgagcatcga aaagaattca gagatcgata ccggtggacg caccccaccc
     1141 agcctgggtc cccaagatga gtcctgcacc accgccagct cctccctggc caaggacact
     1201 tcatcgaccg gtgagaacct gtacttccag ctaagattag ataaaagtaa agtgattaac
     1261 agcgcattag agctgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc
     1321 cagaagctag gtgtagagca gcctacattg tattggcatg taaaaaataa gcgggctttg
     1381 ctcgacgcnt tanccattga gatgttagat aggcaccata ctcacttttg ccctttagaa
     1441 ggggaaagct ggcaagattt tttacgtaat aacgctaaaa gttttagatg tgctttacta
     1501 agtcatcgcg atggagcaaa agtacattta ggtacacggc ctacagaaaa acagtatgaa
     1561 actctcgaaa atcaattagc ctttttatgc caacaaggtt tttcactaga gaatgcatta
     1621 tatgcactca gcgctgtggg gcattttact ttaggttgcg tattggaaga tcaagagcat
     1681 caagtcgcta aagaagaaag ggaaacacct actactgata gtatgccgcc attattacga
     1741 caagctatcg aattatttga tcaccaaggt gcagagccag ccttcttatt cggccttgaa
     1801 ttgatcatat gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc gtacagccgc
     1861 gcgcgtacga aaaacaatta cgggtctacc atcgagggcc tgctcgatct cccggacgac
     1921 gacgcccccg aagaggcggg gctggcggct ccgcgcctgt cctttctccc cgcgggacac
     1981 acgcgcagac tgtcgacggc ccccccgacc gatgtcagcc tgggggacga gctccactta
     2041 gacggcgagg acgtggcgat ggcgcatgcc gacgcgctag acgatttcga tctggacatg
     2101 ttgggggacg gggattcccc gggtccggga tttacccccc acgactccgc cccctacggc
     2161 gctctggata tggccgactt cgagtttgag cagatgttta ccgatgccct tggaattgac
     2221 gagtacggtg ggtagtctag
//
