LOCUS       sequence-478583-       10025 bp    DNA     circular SYN 14-JUL-2025
DEFINITION  TU for the expression of the CRY2(PHR) gene fused to the Etr binding
            domain under the P35S promoter and TNOS terminator in alpha1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10025)
  AUTHORS   Calvache C, Vazquez-Vilar M, Moreno-Gimenez E, Orzaez D
  TITLE     A quantitative autonomous bioluminescence reporter system with a 
            wide dynamic range for Plant Synthetic Biology.
  JOURNAL   Plant Biotechnol J. 2024 Jan;22(1):37-47. doi: 10.1111/pbi.14146. 
            Epub 2023 Oct 26.
  PUBMED    37882352
REFERENCE   2  (bases 1 to 10025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14146";
            journalName: "Plant Biotechnol J"; date: "2024-01"; volume: "22"; 
            issue: "1"; pages: "37-47"
COMMENT     Sequence Label: GB4192
FEATURES             Location/Qualifiers
     source          1..10025
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        616..960
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     904..924
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1661..3154
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     terminator      3181..3433
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3468..3486)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    3674..3698
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4999..5628
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6057..7130
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7196..7390
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7626..7648)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7734..7874
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7789..7808
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7889..7906)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8060..8648)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8140..8159)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8736..9551)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8736..9551)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9459..9478
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    9984..10008
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagactaga gccaagctga tctcctttgc cccggagatc accatggacg actttctcta
       61 tctctacgat ctaggaagaa agttcgacgg agaaggtgac gataccatgt tcaccaccga
      121 taatgagaag attagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga
      181 ggcctacgcg gcaggtctga tcaagacgat ctacccgagt aataatctcc aggagatcaa
      241 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa
      301 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg
      361 cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaagaaag tagttcctac
      421 tgaatcaaag gccatggagt caaaaattca gatcgaggat ctaacagaac tcgccgtgaa
      481 gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt
      541 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
      601 agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt
      661 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta
      721 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcccctg ccgacagtgg
      781 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
      841 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
      901 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
      961 tccggtattt ttacaacaat taccacaaca aaacaaacaa caaacaacat tacaatttac
     1021 tattctagtc gaaatgcccc gccccaagct caagtccgat gacgaggtac tcgaggccgc
     1081 caccgtagtg ctgaagcgtt gcggtcccat agagttcacg ctcagcggag tagcaaagga
     1141 ggtggggctc tcccgcgcag cgttaatcca gcgcttcacc aaccgcgata cgctgctggt
     1201 gaggatgatg gagcgcggcg tcgagcaggt gcggcattac ctgaatgcga taccgatagg
     1261 cgcagggccg caagggctct gggaattttt gcaggtgctc gttcggagca tgaacactcg
     1321 caacgacttc tcggtgaact atctcatctc ctggtacgag ctccaggtgc cggagctacg
     1381 cacgcttgcg atccagcgga accgcgcggt ggtggagggg atccgcaagc gactgccccc
     1441 aggtgctcct gcggcagctg agttgctcct gcactcggtc atcgctggcg cgacgatgca
     1501 gtgggccgtc gatccggatg gtgagctagc tgatcatgtg ctggctcaga tcgctgccat
     1561 cctgtgttta atgtttcccg aacacgacga tttccaactc ctccaggcac atgcgtccgc
     1621 gtacagctta attaacagtg ctggtagtgc tggtggttcg atgaagatgg acaaaaagac
     1681 tatagtttgg tttagaagag atctaaggat tgaggataat cctgcattag cagcagctgc
     1741 tcacgaagga tctgtttttc ctgtcttcat ttggtgtcct gaagaagaag gacagtttta
     1801 tcctggaaga gcttcaagat ggtggatgaa acaatcactt gctcacttat ctcaatcctt
     1861 gaaggctctt ggatctgacc tcactttaat caaaacccac aacacgattt cagcgatctt
     1921 ggattgtatc cgcgttaccg gtgctacaaa agtcgtcttt aaccacctct atgatcctgt
     1981 ttcgttagtt cgggaccata ccgtaaagga gaagctggtg gaacgtggga tctctgtgca
     2041 aagctacaat ggagatctat tgtatgaacc gtgggagata tactgcgaaa agggcaaacc
     2101 ttttacgagt ttcaattctt actggaagaa atgcttagat atgtcgattg aatccgttat
     2161 gcttcctcct ccttggcggt tgatgccaat aactgcagcg gctgaagcga tttgggcgtg
     2221 ttcgattgaa gaactagggc tggagaatga ggccgagaaa ccgagcaatg cgttgttaac
     2281 tagagcttgg tccccaggat ggagcaatgc tgataagtta ctaaatgagt tcatcgagaa
     2341 gcagttgata gattatgcaa agaacagcaa gaaagttgtt gggaattcta cttcactact
     2401 ttctccgtat ctccatttcg gggaaataag cgtcagacac gttttccagt gtgcccggat
     2461 gaaacaaatt atatgggcaa gagataagaa cagtgaagga gaagaaagtg cagatctttt
     2521 tcttagggga atcggtttaa gagagtattc tcggtatata tgtttcaact tcccgtttac
     2581 tcacgagcaa tcgttgttga gtcatcttcg gtttttccct tgggatgctg atgttgataa
     2641 gttcaaggcc tggagacaag gcaggaccgg ttatccgttg gtggatgccg gaatgagaga
     2701 gctttgggct accggatgga tgcataacag aataagagtg attgtttcaa gctttgctgt
     2761 gaagtttctt ctccttccat ggaaatgggg aatgaagtat ttctgggata cacttttgga
     2821 tgctgatttg gaatgtgaca tccttggctg gcagtatatc tctgggagta tccccgatgg
     2881 ccacgagctt gatcgcttgg acaatcccgc gttacaaggc gccaaatatg acccagaagg
     2941 tgagtacata aggcaatggc ttcccgagct tgcgagattg ccaactgaat ggatccatca
     3001 tccatgggac gctcctttaa ccgtactcaa agcttctggt gtggaactcg gaacaaacta
     3061 tgcgaaaccc attgtagaca tcgacacagc tcgtgagcta ctagctaaag ctatttcaag
     3121 aacccgtgaa gcacagatca tgatcggagc agcatgagct tggaatggat cttcgatccc
     3181 gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg
     3241 acgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc
     3301 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac
     3361 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct
     3421 atgttactag atcgggaatt gccaagctaa ttcttgaaga cgaaagggcc tcgtgatacg
     3481 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     3541 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     3601 tccgctcatg agacaataac cctgataaat gcttcaataa tgggaccgac tcgcgctgtc
     3661 atgagacgaa ttctgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt
     3721 agaataatcg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg
     3781 catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct
     3841 atagtgcagt cggcttctga cgttcagtgc agccgtcatc tgaaaacgac atgtcgcaca
     3901 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt
     3961 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca
     4021 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga
     4081 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca
     4141 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg
     4201 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca
     4261 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg
     4321 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg
     4381 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg
     4441 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga
     4501 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga
     4561 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg
     4621 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac
     4681 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac
     4741 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacct
     4801 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg
     4861 gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt
     4921 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca
     4981 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc
     5041 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg
     5101 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa
     5161 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc
     5221 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg
     5281 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc
     5341 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa
     5401 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag
     5461 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac
     5521 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc
     5581 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta
     5641 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca
     5701 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc
     5761 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca
     5821 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa
     5881 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc
     5941 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc
     6001 tgggttgcct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga
     6061 gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg
     6121 agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg
     6181 gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag
     6241 ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc
     6301 cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc
     6361 gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc
     6421 acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac
     6481 tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca
     6541 agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg
     6601 atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg
     6661 ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg
     6721 aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg
     6781 agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc
     6841 tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc
     6901 tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac
     6961 gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt
     7021 caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag
     7081 tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc
     7141 agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggactc tttcctgtgg
     7201 atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga
     7261 acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa
     7321 aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg
     7381 cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc
     7441 ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct
     7501 caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc
     7561 cactcgaccg ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt
     7621 gaaaacctct gacacatgca gctcccggtg acggtcacag cttgtctgta agcggatgcc
     7681 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
     7741 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc
     7801 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     7861 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     7921 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     7981 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     8041 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     8101 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     8161 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     8221 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     8281 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     8341 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     8401 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     8461 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     8521 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     8581 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     8641 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     8701 cacgttaagg gattttggtc atgcattcta ggtgattaga aaaactcatc gagcatcaaa
     8761 tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc
     8821 tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg
     8881 tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata
     8941 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagt
     9001 ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca
     9061 ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagtcg aaatacgcga
     9121 tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc
     9181 agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt
     9241 ttccctggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg
     9301 atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca
     9361 tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca
     9421 tacaatcggt agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca
     9481 tataaatcag catccatgtt ggaatttaat cgcggccttg agcaagacgt ttcccgttga
     9541 atatggctca taacagaact tattatttcc ttcctctttt ctacagtatt taaagatacc
     9601 ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct
     9661 taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc
     9721 gacggagccg attttgaaac cgcggtgatc acaggcagca acgctctgtc atcgttacaa
     9781 tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt
     9841 cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg
     9901 acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg
     9961 tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacat aacgaattcg
    10021 tctca
//
