LOCUS       sequence-478584-        4041 bp    DNA     circular SYN 11-SEP-2025
DEFINITION  Adenoviral-SREBP-1c cleavage-activation reporter system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4041)
  TITLE     Ad-Flag-SREBP-1c vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 11, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pShuttle-CMV-Flag-SREBP-1c
FEATURES             Location/Qualifiers
     source          1..4041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      640..1228
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1129..1148
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1382..1399
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1414..1554
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1480..1499)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1640..1662
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1656..1847)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     rep_origin      2627..3007
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2714..2733)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2924..2945
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(3055..3849)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(3055..3849)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3166..3185)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3776..3795
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gcaagctacc tgctttctct ttgcgcttgc gttttccctt gtccagatag cccagtagct
       61 gacattcatc cggggtcagc accgtttctg cggactggct ttctacgtga aaaggatcta
      121 ggtgaagatc ctttttgata atctcatggc tgcagcaatg gcaacaacgt tgcgcaaact
      181 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      241 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      301 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      361 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
      421 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
      481 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
      541 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
      601 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
      661 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
      721 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
      781 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
      841 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
      901 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
      961 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1021 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1081 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1141 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1201 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1261 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     1321 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1381 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     1441 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     1501 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     1561 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     1621 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     1681 aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg
     1741 tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt
     1801 ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg
     1861 taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg
     1921 atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg
     1981 gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt
     2041 aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac
     2101 ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag
     2161 accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc
     2221 tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc
     2281 ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg
     2341 cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt
     2401 tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg
     2461 ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccgttcccac gccccgcgcc
     2521 acgtcacaaa ctccaccccc tcattatcat attggcttca atccaaaata aggtatatta
     2581 ttgatgatgt taattaagaa ttaattcgat cctgaatggc gaatggacgc gccctgtagc
     2641 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     2701 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     2761 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     2821 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     2881 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     2941 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     3001 atttcggcct attggttaaa aaatgagctg atttaacaaa aattttaaca aaattcagaa
     3061 gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta
     3121 aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc
     3181 caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga
     3241 aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag
     3301 atcctcgccg tcgggcatgc tcgccttgag cctggcgaac agttcggctg gcgcgagccc
     3361 ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc
     3421 tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg
     3481 cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga
     3541 caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac
     3601 aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc
     3661 ctcgtcttgc agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg
     3721 cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca
     3781 gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg
     3841 ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca gagcttgatc ccctgcgcca
     3901 tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc caaccttacc
     3961 agagggcgcc ccagctggca attccggttc gcttgctgtc cataaaaccg cccagtctag
     4021 ctatcgccat gtaagcccac t
//
