LOCUS       sequence-478585-       14087 bp    DNA     circular SYN 14-JUL-2025
DEFINITION  Genome editing in Agrobacterium fabrum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14087)
  AUTHORS   Sun J, Yu X, Tang G, Chen M, Zheng Y, Hu Y, Li Q, Li X, Li N, Li Z, 
            Li Y, Lu N, Tan W, Yang Y, Zeng X, Zhao G, Wang H, Dai L, Zhao G, Ai
            L, Zhao W
  TITLE     A CRISPR/SpCas9M-reporting system for efficient and rapid genome 
            editing in Caulobacter crescentus
  JOURNAL   bioRxiv 2024.12.02.626314
REFERENCE   2  (bases 1 to 14087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.12.02.626314"
COMMENT     Sequence Label: pSJX067
FEATURES             Location/Qualifiers
     source          1..14087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             458..1252
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAHGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(512..531)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1122..1141
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     promoter        1580..1614
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     misc_RNA        1635..1710
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     regulatory      5608..5617
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      5738..5784
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             5924..10027
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5924..10027
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     RBS             9484..9492
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             10043..10756
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     10778..10794
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     10779..10795
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10829..10845)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10829..10845)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(10877..10896)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10878..10896)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10906..10923)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10906..10922)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10915..10937)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(11631..12293)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      12294..13065
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
      121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg
      181 aaaagtgcca cctgggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag
      241 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt
      301 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa
      361 gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc
      421 aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
      481 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
      541 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg catgggcgcc cggttctttt
      601 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
      661 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
      721 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
      781 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
      841 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
      901 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
      961 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     1021 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     1081 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     1141 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     1201 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     1261 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     1321 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     1381 atcctccagc gcggggatct catgctggag ttcttcgccc acccccatgg gcaaatatta
     1441 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     1501 tggcttccat gtcggcagaa tgcttaatga attacaacag tttttatata agctttcagt
     1561 cggcgcgaat gctccacgct tgacagctag ctcagtccta ggtataatac tagtgaaggc
     1621 gcccagatcg aagtgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
     1681 caacttgaaa aagtggcacc gagtcggtgc tttttttgaa ggtgggaatg gtctgcatca
     1741 gatcgagcgc cgggcgcatg ccggcataga tccacttgcg ccgtgccgcc aatatgccga
     1801 gcggtatgcc gatgatcatg cacacggcgc tggcggcgat gaccagggcc agcgtctcag
     1861 tcgtcgcttt ccagtagccg agattgatga tgagcaacag accgagtgcg gtgaagatcg
     1921 gcatgccggc cgagcggcgc gtccaggccg aaagcgccgt cacgatcgcg acgatgatca
     1981 gcggatgcgg cgcctgcagc acgtagagca gggcgttgat gacgctttgg aaaatgaaag
     2041 agagccaatc gaagaaaaaa gcgagattgc cggtcagcca gtcaatggcc tccttggcat
     2101 atcggccgat aggcaggcgg ttttcaggag cgctgagcca ttccacggta agaaaagacc
     2161 tttcgtgaat gagagcaatt ccggcaaaaa tgcagagcgg ttttgcgtcc ggaattgcgc
     2221 agacaacaaa gaagcggagc tttttcagtg atctattgtc accggaattg ctccaataca
     2281 gaagcgacgg ccggcatcgt ctttgccggg cgcggtgcag atccatggcc ttcgatgcga
     2341 gaaaaattcg cgggcaaggt catgcatggg catcgtattt cccactgcat ccgacctgcc
     2401 gcgcaggccg ggtgcagtca tgcatcgtct tgtggcctaa aggccgagag cctttttaac
     2461 ggcaggcagg gcgtccgcgc tgccatcctt tgtcttgacg ttggcgaacc agggctcgat
     2521 cgccgccgga ttggccttca gccaggcggt tgcggccgcc tcgccctcaa gaccgtcatt
     2581 caggatcttg cccatgatct cgttttccat cggcagggaa aactgcaggt tcttaagaaa
     2641 cgcgccgaca ttcgggcatt cttcgacata tcccttgcgg acattggtgt agatcgtcgc
     2701 accgccgaaa ttggggccga agacctcgtc gccgccggtc aggtaggtca gcttgaaatt
     2761 cgcattcatc ggatgcggct cccagccgag gaagacgatc ggctcgccgg atttctcggc
     2821 gcgggccacc tgggaaagca tgccctgctc ggaggattcc accacctcga aggacttcag
     2881 tccgaaactg tcctttgcca ccatgtcgag aatcaggcga ttgccgtcat tgccgggctc
     2941 gatgccgtaa atcttgccgc cgagttcact gctatgtgcg gcaatgtcct tgaaatcctt
     3001 gatgccgagt tcggcaccct tggcattggt cgccagcgta tatttcgcgc cggtcaggtt
     3061 ttccctgagc gtttccaccg atttgtcgtc gcggtagggc gctatgtcac cttccatggt
     3121 cggcatccag ttgccgagga agacgtcgat gtccttgttt ttcaaggacg tataggtgac
     3181 gggtacggag agaagcttga cgtctgtttc gtagccaagg cttttcagaa gcacggtggc
     3241 ggtcgccgtc gtggcggtga tgtcggtcca gccgacatca gaaaaacgca ccgtgccaca
     3301 acttgccggt tcggcggcaa cggccgcgct gaacgtcatg gaggagattg ccgcggcgag
     3361 cgccagacag cgacttctat ttgcaaacat tgtttgctcc ctgttttttc gttcccttgc
     3421 cattatcaat atcttccggc cacaatcaac tcgcgtgcat ttgcgtgctt tggacccccg
     3481 tttgcgacaa aatcgtgaaa atcgccgcct tttcgcaaac ccgattcgct gaaacggcag
     3541 cgtgcggaaa tctgtgtttt tccctcgatg gcacttttca acccgctttt ccgcggtcaa
     3601 aacacgcggc atatggcatc ggtgcaaacg ggaatcgctt tcgtgggacg cgatgtccgc
     3661 aaataggttg aagcattgcg aagcgtccgc agacgcgagg cgctccggga gtggatcccc
     3721 gctgcgtccg gttgccgcat ttgatttttc gcaaagatgg cctgtccgct cctgcgctaa
     3781 gacgacgggg acacattcgc gtggatgtgc tttctctcag gagcagaatt atgctgaaca
     3841 gaactgccaa attcgccggc ctcgccatcg tcgccgctct ttgcgctttt cccgccttgt
     3901 ccgccgaaat cgaagtgaag atgctcaaca agggcagcga cggtcaggcg atggtctttg
     3961 aacctgcaac cgtcaaggcc gccgtcggcg acgtcatcac tttcgtgccg gtcgacaagg
     4021 gccatgatgc ggcagccgtg aaggacatga tccccgaggg tgttgcagag ttcaagggca
     4081 agatgaacga agcggtgaag atcacggtcg agaaggaagg tgcttatgtc gtcaaatgca
     4141 ccccgcatct cggcatgggc atgatcgcgc tcgtcgttgt cggcgatgcg acacccgcca
     4201 atctggacgt cgtcaagaac ggcaaactgc cgaaaaaggc acgcgaccgg ctgaacgagg
     4261 agatcgccaa gctcggcctc taacctcata tcgcctcggt cccgcaccgg cctcgttgat
     4321 ccggcatcat cgaaatgccg caacggccgg cgcggaccgc aggttgagaa aagtttcgca
     4381 atcatgttgt atcccggttg accgggacat atctggaaga cagtaaatgg ccgcggttga
     4441 cagggaacgc caaaggcacc ctgccgaacc gcggattttt cttgccgcat ccggcccacg
     4501 ggcagaggag acgatgacga catgatagac cggatcaccg ctttgattca ggcaggtttc
     4561 agcgctatcg gcaaggctgc cggtcttctc gtcgcatggc ttttatggcc cttcatggcc
     4621 gcgcatggct ggtacagcgg ccgcaactgg ctcatcaagg gcccaattgc tctgatcgcc
     4681 atcctgctgg ccggtttcta cggatatttc gtctggcaaa cccaggtctg gacgaatttc
     4741 gatccggatt acatttcccg ctacaaactt gccgaacgca ccgtgccccc cggacaggaa
     4801 ttgccggccg taactccggc tgctgccgcc ggcgcaaccg gtgttgcagc aaacacctcc
     4861 gcacccgtgt gccagcgctc ggccatcgtg gatgctgccg tcgaccttac cgacttcaac
     4921 gtcaaccaga atgcatggat ttcctccatg ctgctctatc gcctcggcct ctttggtatg
     4981 gactgggaca gcacaccgtt tctcgataac aaggcctcgt tccagcgcgg cgtgaaccag
     5041 gctgtgcgcc gcacctcggt ggagctggta gatacgctcg gccgcgtgcg cggcacctcg
     5101 ggtatcaata acaatctcca ggaagcgcgt ggcaacatgc agttcagcga atattcctgg
     5161 tatttcggcc tcgatccctt cggcccgaag acgccgaccc ccagctatta ccgcgccgca
     5221 atccgcagct tccaggcctt caacggcgaa ctcaccgcct gcaaagccgt cttcgatacc
     5281 cgcgccgaca acctgatcca gttcatcgac cgtatcgcag gcgatatcgg ttccacgtct
     5341 gcaattctgc gcgaacgttc ggaaaactat aatggcggct ggttcgacac gcgtgccgat
     5401 gaccgcttct ggttcgccta tggccagctt tatggttatt acggcgtgct ttccgccgcc
     5461 ggatccgact ttgcgcaggt catccgcgaa cgcaacctga ccaacctctg gaacgacacg
     5521 ctgtcgcaga cccgcgcggc gctgcgcatc cagccggcca tcatttccaa cggcgaggaa
     5581 agcggctggc tcatgccgtc gcacctcgcc accatgggat tttatgttct gcgggtgcgc
     5641 tcgaacctcg tcgagcttcg ctcggttctc gaccgttgat aaggaggccg gcccgctgtc
     5701 cggccggctt actctatcgc gcccagcggc gcggatccaa ataaaacgaa aggctcagtc
     5761 gaaagactgg gcctttcgtt ttatctgttg tttgtcgggc tggactctag ccgaccgact
     5821 gagacgctca caagcgttca attggatcca atcttgacgt ccgtttgatt acgatcaaga
     5881 ttggatccaa taggtcgccg aaccacgatg cgaggaaacg catatggaca agaagtactc
     5941 gatcggcctg gatatcggga cgaacagcgt cgggtgggcc gtgatcacgg acgaatacaa
     6001 ggtgccctcc aagaagttca aggtcctggg gaataccgac cggcactcca tcaagaagaa
     6061 cctcatcggg gccctgctct ttgactccgg ggaaacggcc gaagcgacgc ggctgaagcg
     6121 gaccgcgcgt cgtcgctaca cgcgccggaa gaatcgcatc tgttatctcc aggaaatctt
     6181 cagcaacgag atggccaagg tggatgattc cttcttccac cgcctggagg agtcctttct
     6241 ggtcgaagag gataagaagc atgagcgcca cccgatcttt gggaacatcg tcgacgaagt
     6301 ggcctaccac gaaaagtatc ccacgatcta ccacctgcgt aagaagctcg tggactccac
     6361 cgataaggcg gatctgcggc tgatctacct cgccctggcc cacatgatca agtttcgggg
     6421 ccattttctg atcgaaggcg atctgaatcc ggacaatagc gacgtcgata agctctttat
     6481 ccagctggtg cagacctaca atcaactctt cgaggagaat ccgatcaatg cctccggggt
     6541 ggatgccaag gcgatcctga gcgcgcgtct gtcgaagtcc cgccgcctgg agaacctgat
     6601 cgcccagctc ccgggcgaaa agaagaacgg gctctttggg aatctgatcg ccctcagcct
     6661 ggggctgacc ccgaacttta agtccaattt cgacctcgcg gaagatgcca agctccaact
     6721 ctcgaaggat acgtacgacg atgacctcga caacctgctg gcccaaatcg gcgaccagta
     6781 cgcggacctg ttcctggcgg ccaagaacct gtcggacgcc atcctcctga gcgacatcct
     6841 ccgtgtcaat acggaaatca cgaaggcgcc gctgtccgcc agcatgatca agcggtatga
     6901 tgagcatcat caagacctca ccctgctgaa ggcgctggtc cggcaacagc tgcccgagaa
     6961 gtacaaggag atctttttcg atcaaagcaa gaacggctac gccgggtata tcgacggggg
     7021 ggcctcgcaa gaagagttct acaagtttat caagcccatc ctggagaaga tggatgggac
     7081 ggaggagctc ctcgtcaagc tcaaccggga agatctcctc cgcaagcaac gcacctttga
     7141 taatgggtcc atcccgcatc aaatccacct cggcgagctc catgcgatcc tgcggcggca
     7201 agaggacttc tacccgtttc tcaaggacaa ccgcgagaag atcgagaaga tcctgacgtt
     7261 ccgcatcccc tattatgtcg gccccctggc ccgggggaat tcgcggtttg cctggatgac
     7321 ccgcaagtcc gaagaaacga tcaccccctg gaattttgaa gaggtggtgg acaagggggc
     7381 gagcgcgcag tcctttatcg aacggatgac caatttcgac aagaacctcc ccaacgagaa
     7441 ggtgctcccg aagcattccc tcctgtatga gtactttacc gtctacaacg agctcacgaa
     7501 ggtcaagtat gtcaccgagg gcatgcgcaa gcccgccttc ctctcggggg aacaaaagaa
     7561 ggccatcgtg gacctgctct tcaagaccaa ccgcaaggtc accgtgaagc aactcaagga
     7621 ggactatttc aagaagatcg aatgcttcga tagcgtggag atctccgggg tcgaagaccg
     7681 gtttaacgcc tcgctcggca cgtaccatga tctcctcaag atcatcaagg acaaggactt
     7741 cctcgacaac gaggagaacg aagacatcct ggaagacatc gtgctgaccc tgaccctctt
     7801 cgaggatcgc gagatgatcg aggagcgtct caagacgtac gcgcatctct tcgatgataa
     7861 ggtgatgaag caactcaagc ggcggcgtta cacgggctgg gggcgcctct cccgtaagct
     7921 catcaacggc atccgggaca agcaatcggg gaagaccatc ctggacttcc tgaagtcgga
     7981 cggctttgcc aatcgcaatt ttatgcaact catccacgac gattccctca ccttcaagga
     8041 agatatccag aaggcccagg tgtccgggca aggggactcc ctgcatgaac atatcgccaa
     8101 cctggccggg agccccgcga tcaagaaggg gatcctccag accgtgaagg tggtcgatga
     8161 gctcgtgaag gtcatgggcc ggcataagcc cgaaaatatc gtcatcgaga tggcgcgcga
     8221 gaaccagacc acccaaaagg gccagaagaa ttcgcgggag cgcatgaagc ggatcgaaga
     8281 aggcatcaag gaactcggca gccagatcct gaaggaacat ccggtcgaaa acacgcagct
     8341 ccagaacgag aagctctacc tctactatct ccaaaatggc cgggatatgt acgtggacca
     8401 ggagctcgac atcaatcggc tcagcgacta tgatgtggat catatcgtcc cccaatcctt
     8461 tctcaaggac gacagcatcg ataacaaggt cctgacccgg tcggacaaga atcgtggcaa
     8521 gagcgataac gtcccctccg aggaggtcgt gaagaagatg aagaactatt ggcgccaact
     8581 gctcaacgcc aagctgatca cgcaacggaa gttcgacaac ctcaccaagg ccgagcgcgg
     8641 gggcctctcg gaactggaca aggcggggtt catcaagcgc cagctcgtcg aaacccgtca
     8701 aatcaccaag cacgtggcgc aaatcctgga tagccgcatg aacaccaagt acgatgagaa
     8761 cgacaagctg atccgtgaag tgaaggtgat caccctgaag tccaagctcg tgagcgattt
     8821 ccgcaaggat ttccaatttt ataaggtgcg ggaaatcaat aattaccatc acgcgcatga
     8881 tgcgtatctc aacgccgtgg tcggcacggc cctgatcaag aagtatccca agctggagtc
     8941 ggaattcgtc tacggcgatt acaaggtgta tgacgtccgt aagatgatcg cgaagagcga
     9001 gcaagaaatc gggaaggcca cggcgaagta cttcttctac agcaatatca tgaatttctt
     9061 caagacggag atcacgctgg cgaatggcga aatccgtaag cgcccgctga tcgaaacgaa
     9121 tggcgagacc ggcgaaatcg tgtgggataa ggggcgcgat ttcgcgacgg tccgcaaggt
     9181 cctctcgatg ccgcaggtca acatcgtcaa gaagaccgaa gtccagaccg gggggttttc
     9241 caaggagagc atcctgccga agcgcaacag cgacaagctc atcgcccgca agaaggactg
     9301 ggatcccaag aagtatggcg ggttcgatag cccgaccgtg gcgtactcgg tgctggtggt
     9361 cgcgaaggtc gaaaagggga agtccaagaa gctcaagagc gtcaaggaac tgctggggat
     9421 cacgatcatg gaacgctcct cctttgagaa gaatcccatc gacttcctgg aggcgaaggg
     9481 ctataaggag gtgaagaagg atctcatcat caagctgccg aagtattcgc tcttcgaact
     9541 cgaaaatggg cgcaagcgca tgctggcgtc cgccggcgag ctccaaaagg gcaatgagct
     9601 ggccctgccc tccaagtacg tcaattttct gtacctagcc tcccactatg agaagctgaa
     9661 gggcagcccc gaggataatg aacagaagca actctttgtc gagcaacata agcattacct
     9721 ggatgaaatc atcgagcaaa tcagcgaatt ttccaagcgg gtgatcctcg ccgatgccaa
     9781 cctggacaag gtcctcagcg cgtataacaa gcaccgggac aagcccatcc gtgaacaagc
     9841 ggagaacatc atccatctgt tcacgctcac caacctgggc gcccccgccg cgtttaagta
     9901 ttttgatacc accatcgatc gcaagcgtta tacgtccacc aaggaagtcc tcgatgccac
     9961 gctgatccat caaagcatca ccgggctcta cgagacccgg atcgatctct cccagctggg
    10021 cggcgatcac cggtcggcca ccatgcgtaa aggcgaagag ctgttcactg gtgtcgtccc
    10081 tattctggtg gaactggatg gtgatgtcaa cggtcataag ttttccgtgc gtggcgaggg
    10141 tgaaggtgac gcaactaatg gtaaactgac gctgaagttc atctgtacta ctggtaaact
    10201 gccggtacct tggccgactc tggtaacgac gctgacttat ggtgttcagt gctttgctcg
    10261 ttatccggac catatgaagc agcatgactt cttcaagtcc gccatgccgg aaggctatgt
    10321 gcaggaacgc acgatttcct ttaaggatga cggcacgtac aaaacgcgtg cggaagtgaa
    10381 atttgaaggc gataccctgg taaaccgcat tgagctgaaa ggcattgact ttaaagaaga
    10441 cggcaatatc ctgggccata agctggaata caattttaac agccacaatg tttacatcac
    10501 cgccgataaa caaaaaaatg gcattaaagc gaattttaaa attcgccaca acgtggagga
    10561 tggcagcgtg cagctggctg atcactacca gcaaaacact ccaatcggtg atggtcctgt
    10621 tctgctgcca gacaatcact atctgagcac gcaaagcgtt ctgtctaaag atccgaacga
    10681 gaaacgcgat catatggttc tgctggagtt cgtaaccgca gcgggcatca cgcatggtat
    10741 ggatgaactg tacaaatgaa tgaaaacggg ccccccctcg aggtcgacgg tatcgataag
    10801 cttgatatcg aattcctgca gcccggggga tccactagtt ctagagcggc cgccaccgcg
    10861 gtggagctcc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
    10921 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
    10981 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
    11041 gcgcagcctg aatggcgaat ggaaattgta agcgttaata ttttgttaaa attcgcgtta
    11101 aatttttgtt aaatcagctc attttttaac caataggccg tactgcgatg agtggcaggg
    11161 cggggcgtaa tttttttaag gcagttattg gtgcccttaa acgcctggtt gctacgcctg
    11221 aataagtgat aataagcgga tgaatggcag aaattcgaaa gcaaattcga cccggtcgtc
    11281 ggttcagggc agggtcgtta aatagccgct tatgtctatt gctggtttac cggtttattg
    11341 actaccggaa gcagtgtgac cgtgtgcttc tcaaatgcct gaggccagtt tgctcaggct
    11401 ctccccgtgg aggtaataat tgacgatatg atcatttatt ctgcctccca gagcctgata
    11461 aaaacggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc
    11521 tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg aggcggctac
    11581 agccgatagt ctggaacagc gcacttacgg gttgctgcgc aacccaagtg ctaccggcgc
    11641 ggcagcgtga cccgtgtcgg cggctccaac ggctcgccat cgtccagaaa acacggctca
    11701 tcgggcatcg gcaggcgctg ctgcccgcgc cgttcccatt cctccgtttc ggtcaaggct
    11761 ggcaggtctg gttccatgcc cggaatgccg ggctggctgg gcggctcctc gccggggccg
    11821 gtcggtagtt gctgctcgcc cggatacagg gtcgggatgc ggcgcaggtc gccatgcccc
    11881 aacagcgatt cgtcctggtc gtcgtgatca accaccacgg cggcactgaa caccgacagg
    11941 cgcaactggt cgcggggctg gccccacgcc acgcggtcat tgaccacgta ggccgacacg
    12001 gtgccggggc cgttgagctt cacgacggag atccagcgct cggccaccaa gtccttgact
    12061 gcgtattgga ccgtccgcaa agaacgtccg atgagcttgg aaagtgtctt ctggctgacc
    12121 accacggcgt tctggtggcc catctgcgcc acgaggtgat gcagcagcat tgccgccgtg
    12181 ggtttcctcg caataagccc ggcccacgcc tcatgcgctt tgcgttccgt ttgcacccag
    12241 tgaccgggct tgttcttggc ttgaatgccg atttctctgg actgcgtggc catgcttatc
    12301 tccatgcggt aggggtgccg cacggttgcg gcaccatgcg caatcagctg caacttttcg
    12361 gcagcgcgac aacaattatg cgttgcgtaa aagtggcagt caattacaga ttttctttaa
    12421 cctacgcaat gagctattgc ggggggtgcc gcaatgagct gttgcgtacc cccctttttt
    12481 aagttgttga tttttaagtc tttcgcattt cgccctatat ctagttcttt ggtgcccaaa
    12541 gaagggcacc cctgcggggt tcccccacgc cttcggcgcg gctccccctc cggcaaaaag
    12601 tggcccctcc ggggcttgtt gatcgactgc gcggccttcg gccttgccca aggtggcgct
    12661 gcccccttgg aacccccgca ctcgccgccg tgaggctcgg ggggcaggcg ggcgggcttc
    12721 gcccttcgac tgcccccact cgcataggct tgggtcgttc caggcgcgtc aaggccaagc
    12781 cgctgcgcgg tcgctgcgcg agccttgacc cgccttccac ttggtgtcca accggcaagc
    12841 gaagcgcgca ggccgcaggc cggaggcttt tccccagaga aaattaaaaa aattgatggg
    12901 gcaaggccgc aggccgcgca gttggagccg gtgggtatgt ggtcgaaggc tgggtagccg
    12961 gtgggcaatc cctgtggtca agctcgtggg caggcgcagc ctgtccatca gcttgtccag
    13021 cagggttgtc cacgggccga gcgaagcgag ccagccggtg gccgctcgcg gccatcgtcc
    13081 acatatccac gggctggcaa gggagcgcag cgaccgcgca gggcgaagcc cggagagcaa
    13141 gcccgtaggg cgccgcagcc gccgtaggcg gtcacgactt tgcgaagcaa agtctagtga
    13201 gtatactcaa gcattgagtg gcccgccgga ggcaccgcct tgcgctgccc ccgtcgagcc
    13261 ggttggacac caaaagggag gggcaggcat ggcggcatac gcgatcatgc gatgcaagaa
    13321 gctggcgaaa atgggcaacg tggcggccag tctcaagcac gcctaccgcg agcgcgagac
    13381 gcccaacgct gacgccagca ggacgccaga gaacgagcac tgggcggcca gcagcaccga
    13441 tgaagcgatg ggccgactgc gcgagttgct gccagagaag cggcgcaagg acgctgtgtt
    13501 ggcggtcgag tacgtcatga cggccagccc ggaatggtgg aagtcggcca gccaagaaca
    13561 gcaggcggcg ttcttcgaga aggcgcacaa gtggctggcg gacaagtacg gggcggatcg
    13621 catcgtgacg gccagcatcc accgtgacga aaccagcccg cacatgaccg cgttcgtggt
    13681 gccgctgacg caggacggca ggctgtcggc caaggagttc atcggcaaca aagcgcagat
    13741 gacccgcgac cagaccacgt ttgcggccgc tgtggccgat ctagggctgc aacggggcat
    13801 cgagggcagc aaggcacgtc acacgcgcat tcaggcgttc tacgaggccc tggagcggcc
    13861 accagtgggc cacgtcacca tcagcccgca agcggtcgag ccacgcgcct atgcaccgca
    13921 gggattggcc gaaaagctgg gaatctcaaa gcgcgttgag acgccggaag ccgtggccga
    13981 ccggctgaca aaagcggttc ggcaggggta tgagcctgcc ctacaggccg ccgcaggagc
    14041 gcgtgagatg cgcaagaagg ccgatcaagc ccaagagacg gcccgag
//
