LOCUS       sequence-478604-       16637 bp    DNA     circular SYN 14-JUL-2025
DEFINITION  CCP expression in diverse gram-negative bacteria, with pre-cloned 
            Golden Gate Compatible (BsaI) restriction sites to facilitate 
            promoter swapping. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 8680 to 8714)
  AUTHORS   Armstrong ZR, Alonso J, Stanton V, Patel N, Zogaj X, Cocioba SS, 
            Klose KE
  TITLE     Mobilizable shuttle vectors with fluorescent markers functional 
            across different species of bacteria.
  JOURNAL   Appl Environ Microbiol. 2025 May 12:e0004525. doi: 
            10.1128/aem.00045-25.
  PUBMED    40353662
REFERENCE   2  (bases 1 to 16637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2025 May 12:e0004525. doi: 10.1128/aem.00045-25."
COMMENT     Sequence Label: pKEK3227
FEATURES             Location/Qualifiers
     source          1..16637
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..546
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     CDS             complement(1307..2158)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(2145..2984)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(3495..4466)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     rep_origin      6011..6405
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     CDS             complement(6869..7684)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6869..7684)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7592..7611
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7876..7892
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7876..7892
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(7950..7967)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             8656..8667
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(8680..8714)
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     primer_bind     complement(8721..8737)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      8791..9336
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     CDS             complement(10097..10948)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(10935..11774)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(12285..13256)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     rep_origin      14801..15195
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
ORIGIN
        1 ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa aacgaaaaaa ccgccttgca
       61 gggcggtttt tcgaaggttc tctgagctac caactctttg aaccgaggta actggcttgg
      121 aggagcgcag tcaccaaaac ttgtcctttc agtttagcct taaccggcgc atgacttcaa
      181 gactaactcc tctaaatcaa ttaccagtgg ctgctgccag tggtgctttt gcatgtcttt
      241 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcggac tgaacggggg
      301 gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg
      361 gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca
      421 ggagagcgca cgagggagcc gccaggggga aacgcctggt atctttatag tcctgtcggg
      481 tttcgccacc actgatttga gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta
      541 tggaaaaacg gctttgccgc ggccctctca cttccctgtt aagtatcttc ctggcatctt
      601 ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg ccgcagtcga acgaccgagc
      661 gtagcgagtc agtgagcgag gaagcggaat atatcctgta tcacatattc tgctgacgca
      721 ccggtgcagc cttttttctc ctgccacatg aagcacttca ctgacaccct catcagtgcc
      781 aacatagtaa gccagtatac actccgctag cgctgatgtc cggcggtgct tttgccgtta
      841 cgcaccaccc cgtcagtagc tgaacaggag ggacagcttg gaggtgaagg gcaatcagct
      901 gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc
      961 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     1021 cgggcagtga gcgcaacgca attaatgtaa gttagcgcga attgcaagct ggccgacgcg
     1081 ctgggctacg tcttgctggc gttcgggagc agaagagcat acatctggaa gcaaagccag
     1141 gaaagcggcc tatggagctg tgcggcagcg ctcagtaggc aatttttcaa aatattgtta
     1201 agccttttct gagcatggta tttttcatgg tattaccaat tagcaggaaa ataagccatt
     1261 gaatataaaa gataaaaatg tcttgtttac aatagagtgg ggggggtcag cctgccgcct
     1321 tgggccgggt gatgtcgtac ttgcccgccg cgaactcggt taccgtccag cccagcgcga
     1381 ccagctccgg caacgcctcg cgcacccgct ggcggcgctt gcgcatggtc gaaccactgg
     1441 cctctgacgg ccagacatag ccgcacaagg tatctatgga agccttgccg gttttgccgg
     1501 ggtcgatcca gccacacagc cgctggtgca gcaggcgggc ggtttcgctg tccagcgccc
     1561 gcacctcgtc catgctgatg cgcacatgct ggccgccacc catgacggcc tgcgcgatca
     1621 aggggttcag ggccacgtac aggcgcccgt ccgcctcgtc gctggcgtac tccgacagca
     1681 gccgaaaccc ctgccgcttg cggccattct gggcgatgat ggataccttc caaaggcgct
     1741 cgatgcagtc ctgtatgtgc ttgagcgccc caccactatc gacctctgcc ccgatttcct
     1801 ttgccagcgc ccgatagcta cctttgacca ccatggcatc agcggtgacg gcctcccact
     1861 tgggttccag gaacagccgg agctgccgtc cgccttcggt cttgggttcc gggccaagca
     1921 ctaggccatt aggcccagcc atggccacca gcccttgcag gatgcgcaga tcatcagcgc
     1981 ccagcggctc cgggccgctg aactcgatcc gcttgccgtc gccgtagtca tacgtcacgt
     2041 ccagcttgct gcgcttgcgc tcgccccgct tgagggcacg gaacaggccg ggggccagac
     2101 agtgcgccgg gtcgtgccgg acgtggctga ggctgtgctt gttcttaggc ttcaccacgg
     2161 ggcaccccct tgctcttgcg ctgcctctcc agcacggcgg gcttgagcac cccgccgtca
     2221 tgccgcctga accaccgatc agcgaacggt gcgccatagt tggccttgct cacaccgaag
     2281 cggacgaaga accggcgctg gtcgtcgtcc acaccccatt cctcggcctc ggcgctggtc
     2341 atgctcgaca ggtaggactg ccagcggatg ttatcgacca gtaccgagct gccccggctg
     2401 gcctgctgct ggtcgcctgc gcccatcatg gccgcgccct tgctggcatg gtgcaggaac
     2461 acgatagagc acccggtatc ggcggcgatg gcctccatgc gaccgatgac ctgggccatg
     2521 gggccgctgg cgttttcttc ctcgatgtgg aaccggcgca gcgtgtccag caccatcagg
     2581 cggcggccct cggcggcgcg cttgaggccg tcgaaccact ccggggccat gatgttgggc
     2641 aggctgccga tcagcggctg gatcagcagg ccgtcagcca cggcttgccg ttcctcggcg
     2701 ctgaggtgcg ccccaagggc gtgcaggcgg tgatgaatgg cggtgggcgg gtcttcggcg
     2761 ggcaggtaga tcaccgggcc ggtgggcagt tcgcccacct ccagcagatc cggcccgcct
     2821 gcaatctgtg cggccagttg cagggccagc atggatttac cggcaccacc gggcgacacc
     2881 agcgccccga ccgtaccggc caccatgttg ggcaaaacgt agtccagcgg tggcggcgct
     2941 gctgcgaacg cctccagaat attgataggc ttatgggtag ccattgattg cctcctttgc
     3001 aggcagttgg tggttaggcg ctggcggggt cactaccccc gccctgcgcc gctctgagtt
     3061 cttccaggca ctcgcgcagc gcctcgtatt cgtcgtcggt cagccagaac ttgcgctgac
     3121 gcatcccttt ggccttcatg cgctcggcat atcgcgcttg gcgtacagcg tcagggctgg
     3181 ccagcaggtc gccggtctgc ttgtcctttt ggtctttcat atcagtcacc gagaaacttg
     3241 ccggggccga aaggcttgtc ttcgcggaac aaggacaagg tgcagccgtc aaggttaagg
     3301 ctggccatat cagcgactga aaagcggcca gcctcggcct tgtttgacgt ataaccaaag
     3361 ccaccgggca accaatagcc cttgtcactt ttgatcaggt agaccgaccc tgaagcgctt
     3421 ttttcgtatt ccataaaacc cccttctgtg cgtgagtact catagtataa caggcgtgag
     3481 taccaacgca agcactacat gctgaaatct ggcccgcccc tgtccatgcc tcgctggcgg
     3541 ggtgccggtg cccgtgccag ctcggcccgc gcaagctgga cgctgggcag acccatgacc
     3601 ttgctgacgg tgcgctcgat gtaatccgct tcgtggccgg gcttgcgctc tgccagcgct
     3661 gggctggcct cggccatggc cttgccgatt tcctcggcac tgcggccccg gctggccagc
     3721 ttctgcgcgg cgataaagtc gcacttgctg aggtcatcac cgaagcgctt gaccagcccg
     3781 gccatctcgc tgcggtactc gtccagcgcc gtgcgccggt ggcggctaag ctgccgctcg
     3841 ggcagttcga ggctggccag cctgcgggcc ttctcctgct gccgctgggc ctgctcgatc
     3901 tgctggccag cctgctgcac cagcgccggg ccagcggtgg cggtcttgcc cttggattca
     3961 cgcagcagca cccacggctg ataaccggcg cgggtggtgt gcttgtcctt gcggttggtg
     4021 aagcccgcca agcggccata gtggcggctg tcggcgctgg ccgggtcggc gtcgtactcg
     4081 ctggccagcg tccgggcaat ctgcccccga agttcaccgc ctgcggcgtc ggccaccttg
     4141 acccatgcct gatagttctt cgggctggtt tccactacca gggcaggctc ccggccctcg
     4201 gctttcatgt catccaggtc aaactcgctg aggtcgtcca ccagcaccag accatgccgc
     4261 tcctgctcgg cgggcctgat atacacgtca ttgccctggg cattcatccg cttgagccat
     4321 ggcgtgttct ggagcacttc ggcggctgac cattcccggt tcatcatctg gccggtggtg
     4381 gcgtccctga cgccgatatc gaagcgctca cagcccatgg ccttgagctg tcggcctatg
     4441 gcctgcaaag tcctgtcgtt cttcatcggg ccaccaagcg cagccagatc gagccgtcct
     4501 cggttgtcag tggcgtcagg tcgagcaaga gcaacgatgc gatcagcagc accaccgtag
     4561 gcatcatgga agccagcatc acggttagcc atagcttcca gtgccacccc cgcgacgcgc
     4621 tccgggcgct ctgcgcggcg ctgctcacct cggcggctac ctcccgcaac tctttggcca
     4681 gctccaccca tgccgcccct gtctggcgct gggctttcag ccactccgcc gcctgcgcct
     4741 cgctggcctg ctgggtctgg ctcatgacct gccgggcttc gtcggccagt gtcgccatgc
     4801 tctgggccag cggttcgatc tgctccgcta actcgttgat gcctctggat ttcttcactc
     4861 tgtcgattgc gttcatggtc tattgcctcc cggtattcct gtaagtcgat gatctgggcg
     4921 ttggcggtgt cgatgttcag ggccacgtct gcccggtcgg tgcggatgcc ccggccttcc
     4981 atctccacca cgttcggccc caggtgaaca ccgggcaggc gctcgatgcc ctgcgcctca
     5041 agtgttctgt ggtcaatgcg ggcgtcgtgg ccagcccgct ctaatgcccg gttggcatgg
     5101 tcggcccatg cctcgcgggt ctgctcaagc catgccttgg gcttgagcgc ttcggtcttc
     5161 tgtgccccgc ccttctccgg ggtcttgccg ttgtaccgct tgaaccactg agcggcgggc
     5221 cgctcgatgc cgtcattgat ccgctcggag atcatcaggt ggcagtgcgg gttctcgccg
     5281 ccaccggcat ggatggccag cgtatacggc aggcgctcgg caccggtcag gtgctgggcg
     5341 aactcggacg ccagcgcctt ctgctggtcg agggtcagct cgaccggcag ggcaaattcg
     5401 acctccttga acagccgccc attggcgcgt tcatacaggt cggcagcatc ccagtagtcg
     5461 gcgggccgct cgacgaactc cggcatgtgc ccggattcgg cgtgcaagac ttcatccatg
     5521 tcgcgggcat acttgccttc gcgctggatg tagtcggcct tggccctggc cgattggccg
     5581 cccgacctgc tgccggtttt cgccgtaagg tgataaatcg ccatgctgcc tcgctgttgc
     5641 ttttgctttt cggctccatg atagcctacg agacagcaca ttaacaatgg ggtgtcaaga
     5701 tggttaaggg gagcaacaag gcggcggatc ggctggccaa gctcgaagaa caacgagcgc
     5761 gaatcaatgc cgaaattcag cgggtgcggg caagggaaca gcagcaagag cgcaagaacg
     5821 aaacaaggcg caaggtgctg gtgggggcca tgattttggc caaggtgaac agcagcgagt
     5881 ggccggagga tcggctcatg gcggcaatgg atgcgtacct tgaacgcgac cacgaccgcg
     5941 ccttgttcgg tctgccgcca cgccagaagg atgagccggg ctgaatgatc gaccgagaca
     6001 ggccctgcgg ggctgcacac gcgcccccac ccttcgggta gggggaaagg ccgctaaagc
     6061 ggctaaaagc gctccagcgt atttctgcgg ggtttggtgt ggggtttagc gggctttgcc
     6121 cgcctttccc cctgccgcgc agcggtgggg cggtgtgtag cctagcgcag cgaatagacc
     6181 agctatccgg cctctggccg ggcatattgg gcaagggcag cagcgcccca caagggcgct
     6241 gataaccgcg cctagtggat tattcttaga taatcatgga tggatttttc caacaccccg
     6301 ccagcccccg cccctgctgg gtttgcaggt ttgggggcgt gacagttatt gcaggggttc
     6361 gtgacagtta ttgcaggggg gcgtgacagt tattgcaggg gttcgtgaca gttagtacgg
     6421 gagtgacggg cactggctgg caatgtctag caacggcagg catttcggct gagggtaaaa
     6481 gaactttccg ctaagcgata gactgtatgt aaacacagta ttgcaaggac gcggaacatg
     6541 cctcatgtgg cggccaggac ggccagccgg gatcgggata ctggtcgtta ccagagccac
     6601 cgacccgagc aaacccttct ctatcagatc gttgacgagt attacccggc attcgctgcg
     6661 cttatggcag agcagggaaa ggaattgccg ggctatgtgc aacgggaatt tgaagaattt
     6721 ctccaatgcg ggcggctgga gcatggcttt ctacgggttc gctgcgagtc ttgccacgcc
     6781 gagcacctgg tcgctttcag aaatcaatct aaagtatata tgagtaaact tggtctgaca
     6841 ggctcgcgct ttgttagcag ccggatcctt agaaaaactc atcgagcatc aaatgaaact
     6901 gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg
     6961 aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga
     7021 ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat
     7081 caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa agcttatgca
     7141 tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat
     7201 caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt
     7261 taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat
     7321 caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg
     7381 ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg
     7441 gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg
     7501 caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc
     7561 gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat
     7621 cagcatccat gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt tgaatatggc
     7681 tcatatgttt agtctccttt ttttgttata aatattttat acacaaaaat cttcgttatt
     7741 taaataagtt ttatagctat tttttcaaga tacaaaacta ttaattagtt ataattaggt
     7801 tattagctta aaattataaa ccaaatacga tgagtgacaa cccaaaagat ctcgatcctc
     7861 tacgccgttg gggagcagga aacagctatg acggtaccaa aaaaaacccc gccctgtcag
     7921 gggcggggtt tttttttgag ctcttatcaa tggtgatggt ggtgatgtgt ttccttgaca
     7981 tcgactgtga agtgtttagc aactgcgtgt tcctggattt gaaatccatc agctacttct
     8041 ttcttcacca ggacgtgttc aataatgtgg ttggggggca tctttacggc cttgtgggcc
     8101 ttgtatgatg ttgtaaattg gcatttaagt ctatgtcctc cttccaataa taagaacatg
     8161 gtaacgtccc ccttcagtat cccatcacaa gcagtgataa gttctgaact gggttcccag
     8221 ttaagtgtct tcttctgcat tacaggtcca ttagctggga agttgactcc gtgaaaggtg
     8281 gaattgtgga taaaacaatc tttttccaga cttatgtgag cagaggcggt cccgcagccg
     8341 ccatcctcga acattaatgt acgctcccaa gaatagccct cgggcaggct ttgtttaaag
     8401 taatctggca tatcttcggg gtaatcgcaa aaacatcggt tgccataggt gaaggtagca
     8461 ctcagtatat caaaggcaaa agggagagga cccccctttg tgactctgag tttcagggtt
     8521 tttttaccct cgtaaggttt gcctgcacct tcaccttcaa tggtaaattg atgaccatta
     8581 acgcaaccgt ccatgtggaa tcgcattctg acatctcttg gcaaaacttg ttttgacagg
     8641 gaagccatgg gactctttct cctctttaat cgagaccaag ctagcactgt acctaggact
     8701 gagctagccg tcaagaattc actggccgtc gttttacggt ctcagtcgac tactctagaa
     8761 atattttatc tgattaataa gatgatcttc ttgagatcgt tttggtctgc gcgtaatctc
     8821 ttgctctgaa aacgaaaaaa ccgccttgca gggcggtttt tcgaaggttc tctgagctac
     8881 caactctttg aaccgaggta actggcttgg aggagcgcag tcaccaaaac ttgtcctttc
     8941 agtttagcct taaccggcgc atgacttcaa gactaactcc tctaaatcaa ttaccagtgg
     9001 ctgctgccag tggtgctttt gcatgtcttt ccgggttgga ctcaagacga tagttaccgg
     9061 ataaggcgca gcggtcggac tgaacggggg gttcgtgcat acagtccagc ttggagcgaa
     9121 ctgcctaccc ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca taacagcgga
     9181 atgacaccgg taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc gccaggggga
     9241 aacgcctggt atctttatag tcctgtcggg tttcgccacc actgatttga gcgtcagatt
     9301 tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg gctttgccgc ggccctctca
     9361 cttccctgtt aagtatcttc ctggcatctt ccaggaaatc tccgccccgt tcgtaagcca
     9421 tttccgctcg ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag gaagcggaat
     9481 atatcctgta tcacatattc tgctgacgca ccggtgcagc cttttttctc ctgccacatg
     9541 aagcacttca ctgacaccct catcagtgcc aacatagtaa gccagtatac actccgctag
     9601 cgctgatgtc cggcggtgct tttgccgtta cgcaccaccc cgtcagtagc tgaacaggag
     9661 ggacagcttg gaggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac
     9721 caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     9781 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtaa
     9841 gttagcgcga attgcaagct ggccgacgcg ctgggctacg tcttgctggc gttcgggagc
     9901 agaagagcat acatctggaa gcaaagccag gaaagcggcc tatggagctg tgcggcagcg
     9961 ctcagtaggc aatttttcaa aatattgtta agccttttct gagcatggta tttttcatgg
    10021 tattaccaat tagcaggaaa ataagccatt gaatataaaa gataaaaatg tcttgtttac
    10081 aatagagtgg ggggggtcag cctgccgcct tgggccgggt gatgtcgtac ttgcccgccg
    10141 cgaactcggt taccgtccag cccagcgcga ccagctccgg caacgcctcg cgcacccgct
    10201 ggcggcgctt gcgcatggtc gaaccactgg cctctgacgg ccagacatag ccgcacaagg
    10261 tatctatgga agccttgccg gttttgccgg ggtcgatcca gccacacagc cgctggtgca
    10321 gcaggcgggc ggtttcgctg tccagcgccc gcacctcgtc catgctgatg cgcacatgct
    10381 ggccgccacc catgacggcc tgcgcgatca aggggttcag ggccacgtac aggcgcccgt
    10441 ccgcctcgtc gctggcgtac tccgacagca gccgaaaccc ctgccgcttg cggccattct
    10501 gggcgatgat ggataccttc caaaggcgct cgatgcagtc ctgtatgtgc ttgagcgccc
    10561 caccactatc gacctctgcc ccgatttcct ttgccagcgc ccgatagcta cctttgacca
    10621 ccatggcatc agcggtgacg gcctcccact tgggttccag gaacagccgg agctgccgtc
    10681 cgccttcggt cttgggttcc gggccaagca ctaggccatt aggcccagcc atggccacca
    10741 gcccttgcag gatgcgcaga tcatcagcgc ccagcggctc cgggccgctg aactcgatcc
    10801 gcttgccgtc gccgtagtca tacgtcacgt ccagcttgct gcgcttgcgc tcgccccgct
    10861 tgagggcacg gaacaggccg ggggccagac agtgcgccgg gtcgtgccgg acgtggctga
    10921 ggctgtgctt gttcttaggc ttcaccacgg ggcaccccct tgctcttgcg ctgcctctcc
    10981 agcacggcgg gcttgagcac cccgccgtca tgccgcctga accaccgatc agcgaacggt
    11041 gcgccatagt tggccttgct cacaccgaag cggacgaaga accggcgctg gtcgtcgtcc
    11101 acaccccatt cctcggcctc ggcgctggtc atgctcgaca ggtaggactg ccagcggatg
    11161 ttatcgacca gtaccgagct gccccggctg gcctgctgct ggtcgcctgc gcccatcatg
    11221 gccgcgccct tgctggcatg gtgcaggaac acgatagagc acccggtatc ggcggcgatg
    11281 gcctccatgc gaccgatgac ctgggccatg gggccgctgg cgttttcttc ctcgatgtgg
    11341 aaccggcgca gcgtgtccag caccatcagg cggcggccct cggcggcgcg cttgaggccg
    11401 tcgaaccact ccggggccat gatgttgggc aggctgccga tcagcggctg gatcagcagg
    11461 ccgtcagcca cggcttgccg ttcctcggcg ctgaggtgcg ccccaagggc gtgcaggcgg
    11521 tgatgaatgg cggtgggcgg gtcttcggcg ggcaggtaga tcaccgggcc ggtgggcagt
    11581 tcgcccacct ccagcagatc cggcccgcct gcaatctgtg cggccagttg cagggccagc
    11641 atggatttac cggcaccacc gggcgacacc agcgccccga ccgtaccggc caccatgttg
    11701 ggcaaaacgt agtccagcgg tggcggcgct gctgcgaacg cctccagaat attgataggc
    11761 ttatgggtag ccattgattg cctcctttgc aggcagttgg tggttaggcg ctggcggggt
    11821 cactaccccc gccctgcgcc gctctgagtt cttccaggca ctcgcgcagc gcctcgtatt
    11881 cgtcgtcggt cagccagaac ttgcgctgac gcatcccttt ggccttcatg cgctcggcat
    11941 atcgcgcttg gcgtacagcg tcagggctgg ccagcaggtc gccggtctgc ttgtcctttt
    12001 ggtctttcat atcagtcacc gagaaacttg ccggggccga aaggcttgtc ttcgcggaac
    12061 aaggacaagg tgcagccgtc aaggttaagg ctggccatat cagcgactga aaagcggcca
    12121 gcctcggcct tgtttgacgt ataaccaaag ccaccgggca accaatagcc cttgtcactt
    12181 ttgatcaggt agaccgaccc tgaagcgctt ttttcgtatt ccataaaacc cccttctgtg
    12241 cgtgagtact catagtataa caggcgtgag taccaacgca agcactacat gctgaaatct
    12301 ggcccgcccc tgtccatgcc tcgctggcgg ggtgccggtg cccgtgccag ctcggcccgc
    12361 gcaagctgga cgctgggcag acccatgacc ttgctgacgg tgcgctcgat gtaatccgct
    12421 tcgtggccgg gcttgcgctc tgccagcgct gggctggcct cggccatggc cttgccgatt
    12481 tcctcggcac tgcggccccg gctggccagc ttctgcgcgg cgataaagtc gcacttgctg
    12541 aggtcatcac cgaagcgctt gaccagcccg gccatctcgc tgcggtactc gtccagcgcc
    12601 gtgcgccggt ggcggctaag ctgccgctcg ggcagttcga ggctggccag cctgcgggcc
    12661 ttctcctgct gccgctgggc ctgctcgatc tgctggccag cctgctgcac cagcgccggg
    12721 ccagcggtgg cggtcttgcc cttggattca cgcagcagca cccacggctg ataaccggcg
    12781 cgggtggtgt gcttgtcctt gcggttggtg aagcccgcca agcggccata gtggcggctg
    12841 tcggcgctgg ccgggtcggc gtcgtactcg ctggccagcg tccgggcaat ctgcccccga
    12901 agttcaccgc ctgcggcgtc ggccaccttg acccatgcct gatagttctt cgggctggtt
    12961 tccactacca gggcaggctc ccggccctcg gctttcatgt catccaggtc aaactcgctg
    13021 aggtcgtcca ccagcaccag accatgccgc tcctgctcgg cgggcctgat atacacgtca
    13081 ttgccctggg cattcatccg cttgagccat ggcgtgttct ggagcacttc ggcggctgac
    13141 cattcccggt tcatcatctg gccggtggtg gcgtccctga cgccgatatc gaagcgctca
    13201 cagcccatgg ccttgagctg tcggcctatg gcctgcaaag tcctgtcgtt cttcatcggg
    13261 ccaccaagcg cagccagatc gagccgtcct cggttgtcag tggcgtcagg tcgagcaaga
    13321 gcaacgatgc gatcagcagc accaccgtag gcatcatgga agccagcatc acggttagcc
    13381 atagcttcca gtgccacccc cgcgacgcgc tccgggcgct ctgcgcggcg ctgctcacct
    13441 cggcggctac ctcccgcaac tctttggcca gctccaccca tgccgcccct gtctggcgct
    13501 gggctttcag ccactccgcc gcctgcgcct cgctggcctg ctgggtctgg ctcatgacct
    13561 gccgggcttc gtcggccagt gtcgccatgc tctgggccag cggttcgatc tgctccgcta
    13621 actcgttgat gcctctggat ttcttcactc tgtcgattgc gttcatggtc tattgcctcc
    13681 cggtattcct gtaagtcgat gatctgggcg ttggcggtgt cgatgttcag ggccacgtct
    13741 gcccggtcgg tgcggatgcc ccggccttcc atctccacca cgttcggccc caggtgaaca
    13801 ccgggcaggc gctcgatgcc ctgcgcctca agtgttctgt ggtcaatgcg ggcgtcgtgg
    13861 ccagcccgct ctaatgcccg gttggcatgg tcggcccatg cctcgcgggt ctgctcaagc
    13921 catgccttgg gcttgagcgc ttcggtcttc tgtgccccgc ccttctccgg ggtcttgccg
    13981 ttgtaccgct tgaaccactg agcggcgggc cgctcgatgc cgtcattgat ccgctcggag
    14041 atcatcaggt ggcagtgcgg gttctcgccg ccaccggcat ggatggccag cgtatacggc
    14101 aggcgctcgg caccggtcag gtgctgggcg aactcggacg ccagcgcctt ctgctggtcg
    14161 agggtcagct cgaccggcag ggcaaattcg acctccttga acagccgccc attggcgcgt
    14221 tcatacaggt cggcagcatc ccagtagtcg gcgggccgct cgacgaactc cggcatgtgc
    14281 ccggattcgg cgtgcaagac ttcatccatg tcgcgggcat acttgccttc gcgctggatg
    14341 tagtcggcct tggccctggc cgattggccg cccgacctgc tgccggtttt cgccgtaagg
    14401 tgataaatcg ccatgctgcc tcgctgttgc ttttgctttt cggctccatg atagcctacg
    14461 agacagcaca ttaacaatgg ggtgtcaaga tggttaaggg gagcaacaag gcggcggatc
    14521 ggctggccaa gctcgaagaa caacgagcgc gaatcaatgc cgaaattcag cgggtgcggg
    14581 caagggaaca gcagcaagag cgcaagaacg aaacaaggcg caaggtgctg gtgggggcca
    14641 tgattttggc caaggtgaac agcagcgagt ggccggagga tcggctcatg gcggcaatgg
    14701 atgcgtacct tgaacgcgac cacgaccgcg ccttgttcgg tctgccgcca cgccagaagg
    14761 atgagccggg ctgaatgatc gaccgagaca ggccctgcgg ggctgcacac gcgcccccac
    14821 ccttcgggta gggggaaagg ccgctaaagc ggctaaaagc gctccagcgt atttctgcgg
    14881 ggtttggtgt ggggtttagc gggctttgcc cgcctttccc cctgccgcgc agcggtgggg
    14941 cggtgtgtag cctagcgcag cgaatagacc agctatccgg cctctggccg ggcatattgg
    15001 gcaagggcag cagcgcccca caagggcgct gataaccgcg cctagtggat tattcttaga
    15061 taatcatgga tggatttttc caacaccccg ccagcccccg cccctgctgg gtttgcaggt
    15121 ttgggggcgt gacagttatt gcaggggttc gtgacagtta ttgcaggggg gcgtgacagt
    15181 tattgcaggg gttcgtgaca gttagtacgg gagtgacggg cactggctgg caatgtctag
    15241 caacggcagg catttcggct gagggtaaaa gaactttccg ctaagcgata gactgtatgt
    15301 aaacacagta ttgcaaggac gcggaacatg cctcatgtgg cggccaggac ggccagccgg
    15361 gatcgggata ctggtcgtta ccagagccac cgacccgagc aaacccttct ctatcagatc
    15421 gttgacgagt attacccggc attcgctgcg cttatggcag agcagggaaa ggaattgccg
    15481 ggctatgtgc aacgggaatt tgaagaattt ctccaatgcg ggcggctgga gcatggcttt
    15541 ctacgggttc gctgcgagtc ttgccacgcc gagcacctgg tcgctttcag aaatcaatct
    15601 aaagtatata tgagtaaact tggtctgaca ggctcgcgaa ggcgtctttg ttagcagccg
    15661 gatccctgca gttacttatt aaataattta tagctattga aaagagataa gaattattca
    15721 aactaatatt gtttaaaaag tcaattcctg catgttttaa ggaactgtta aattgatttt
    15781 gtcaagatct tcttgtattc tgtgttaacc cacttcataa cgaaataatt atacttctgt
    15841 ttatctttgt gtgatattct tgaatttttt ctagtgaatc tgataagtga gcgactcact
    15901 ctaggtttag aatgaaaata ttctcttgga accatactta atatagaaat atcaacatct
    15961 gccattaaaa ataatgccaa tgagcgtttt gtatttaata atcttttagc aaacccgtat
    16021 tccacgatta aataaatctc atcagctata ctatcaaaaa caattttgcg tattatatcc
    16081 gtacttatgt tataaggtat attaccaaat attttatagg attggttttt aggaaattta
    16141 aactgcaata tatccttgtt taaaacttgg aaattatcgt gatcaacaag tttattttct
    16201 gtagttttgc ataatttatg gtctatttca atgccagtta cgaaattaca cctctgtact
    16261 aattcaaggg taaaatgccc tttgcctgag ccgatttcaa agatattatc atgttcattt
    16321 aatcttatat ttgtcattat tttatctata ttatgttttg aagtaataaa gttttgactg
    16381 tgttttatat ttttctcgct catatgttta gtctcctttt tttgttataa atattttata
    16441 cacaaaaatc ttcgttattt aaataagttt tatagctatt ttttcaagat acaaaactat
    16501 taattagtta taattaggtt attagcttaa aattataaac caaatacgat gagtgacaac
    16561 ccaaaagatc tcgatcctct acgccgcagc tgcgctatac tctagaaata ttttatctga
    16621 ttaataagat gatcttc
//
