LOCUS       sequence-478622-        5029 bp    DNA     circular SYN 15-JUL-2025
DEFINITION  Express Cyclin L2 alpha.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Loyer P, Trembley JH, Grenet JA, Busson A, Corlu A, Zhao W, Kocak M,
            Kidd VJ, Lahti JM
  TITLE     Characterization of cyclin L1 and L2 interactions with CDK11 and 
            splicing factors: influence of cyclin L isoforms on splice site 
            selection.
  JOURNAL   J Biol Chem. 2008 Mar 21;283(12):7721-32. doi: 
            10.1074/jbc.M708188200. Epub 2008 Jan 23.
  PUBMED    18216018
REFERENCE   2  (bases 1 to 5029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M708188200"; journalName: "J Biol Chem"; date: 
            "2008-03-21- 21"; volume: "283"; issue: "12"; pages: "7721-32"
COMMENT     Sequence Label: pFLEX-FLAG-human Cyclin L2 alpha
FEATURES             Location/Qualifiers
     source          1..5029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1204..1222
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1292..1312)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(1297..1626,1627..1641,1642..1910)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      1477..1612
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             1642..1910
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          1954..2050
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             2104..2127
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    3730..3864
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3754..3773)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3808..3827
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4007..4029)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4115..4255
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4170..4189
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4270..4287)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4441..5029)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4521..4540)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     1201 aaaaataggc gtatcacgag gccctttcgt cttcaagaat tggtcgacca attctcatgt
     1261 ttgacagctt atcatcgata agcttggctg tggaatgtgt gtcagttagg gtgtggaaag
     1321 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     1381 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     1441 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     1501 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     1561 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     1621 tgcaaaaagc tcctcgatcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc
     1681 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc
     1741 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg
     1801 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt
     1861 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctcctc
     1921 gatcgaggaa ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt gtcttttatt
     1981 tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg gatgttgcct
     2041 ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgctct agccctcgcc
     2101 atggactaca aggacgacga tgacaagtct cgagcaattg aattcatggc ggcggcggcg
     2161 gcggcggctg gtgctgcagg gtcggcagct cccgcggcag cggccggcgc cccgggatct
     2221 gggggcgcac cctcagggtc gcagggggtg ctgatcgggg acaggctgta ctccggggtg
     2281 ctcatcacct tggagaactg cctcctgcct gacgacaagc tccgtttcac gccgtccatg
     2341 tcgagcggcc tcgacaccga cacagagacc gacctccgcg tggtgggctg cgagctcatc
     2401 caggcggccg gtatcctgct ccgcctgccg caggtggcca tggctaccgg gcaggtgttg
     2461 ttccagcggt tcttttatac caagtccttc gtgaagcact ccatggagca tgtgtcaatg
     2521 gcctgtgtcc acctggcttc caagatagaa gaggccccaa gacgcatacg ggacgtcatc
     2581 aatgtgtttc accgccttcg acagctgaga gacaaaaaga agcccgtgcc tctactactg
     2641 gatcaagatt atgttaattt aaagaaccaa attataaagg cggaaagacg agttctcaaa
     2701 gagttgggtt tctgcgtcca tgtgaagcat cctcataaga taatcgttat gtaccttcag
     2761 gtgttagagt gtgagcgtaa ccaacacctg gtccagacct catggaatta catgaacgac
     2821 agccttcgca ccgacgtctt cgtgcggttc cagccagaga gcatcgcctg tgcctgcatt
     2881 tatcttgctg cccggacgct ggagatccct ttgcccaatc gtccccattg gtttctcttg
     2941 tttggagcaa ctgaagaaga aattcaggaa atctgcttaa agatcttgca gctttatgct
     3001 cggaaaaagg ttgatctcac acacctggag ggtgaagtgg aaaaaagaaa gcacgctatc
     3061 gaagaggcaa aggcccaagc ccggggcctg ttgcctgggg gcacacaggt gctggatggt
     3121 acctcggggt tctctcctgc ccccaagctg gtggaatccc ccaaagaagg taaagggagc
     3181 aagccttccc cactgtctgt gaagaacacc aagaggaggc tggagggcgc caagaaagcc
     3241 aaggcggaca gccccgtgaa cggcttgcca aaggggcgag agagtcggag tcggagccgg
     3301 agccgtgagc agagctactc gaggtcccca tcccgatcag cgtctcctaa gaggaggaaa
     3361 agtgacagcg gctccacatc tggtgggtcc aagtcgcaga gccgctcccg gagcaggagt
     3421 gactccccac cgagacaggc cccccgcagc gctccctaca aaggctctga gattcggggc
     3481 tcccggaagt ccaaggactg caagtacccc cagaagccac acaagtctcg gagccggagt
     3541 tcttcccgtt ctcgaagcag gtcacgggag cgggcggata atccgggaaa atacaagaag
     3601 aaaagtcatt actacagaga tcagcgacga gagcgctcga ggtcgtatga acgcacaggc
     3661 cgtcgctatg agcgggacca ccctgggcac agcaggcatc ggaggtgaga attcctgatt
     3721 gattgaccgg atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     3781 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     3841 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     3901 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
     3961 gattatgatc cggctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     4021 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     4081 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg
     4141 atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     4201 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc
     4261 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     4321 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4381 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4441 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4501 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4561 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4621 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4681 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4741 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4801 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4861 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     4921 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     4981 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaa
//
