LOCUS       sequence-478688-        3564 bp    DNA     circular SYN 15-JUL-2025
DEFINITION  This plasmid contains a human pancreatic islet active enhancer, 
            which can be shuttled into a Gateway destination vector, such as 
            pGL4.23-Gateway..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Pasquali L, Gaulton KJ, Rodriguez-Segui SA, Mularoni L, 
            Miguel-Escalada I, Akerman I, Tena JJ, Moran I, Gomez-Marin C, van 
            de Bunt M, Ponsa-Cobas J, Castro N, Nammo T, Cebola I, 
            Garcia-Hurtado J, Maestro MA, Pattou F, Piemonti L, Berney T, Gloyn 
            AL, Ravassard P, Gomez-Skarmeta JL, Muller F, McCarthy MI, Ferrer J
  TITLE     Pancreatic islet enhancer clusters enriched in type 2 diabetes 
            risk-associated variants.
  JOURNAL   Nat Genet. 2014 Feb;46(2):136-43. doi: 10.1038/ng.2870. Epub 2014 
            Jan 12.
  PUBMED    24413736
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.2870"; 
            journalName: "Nat Genet"; date: "2014-02"; volume: "46"; issue: "2";
            pages: "136-43"
COMMENT     Sequence Label: pENTR D-TOPO-C3_38
FEATURES             Location/Qualifiers
     source          1..3564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     19..38
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     140..156
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     140..156
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     161..180
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        161..179
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    197..296
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1317..1416)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1432..1449)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1432..1448)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1441..1463)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      1512..1598
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1690..1717
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1876..1893)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2047..2635)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2127..2146)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2782..3564,1..27))
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3505..3524
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 cgacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta tgtaagcaga
       61 cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc agagattttg
      121 agacacgggc cagagctgcc aggaaacagc tatgaccatg taatacgact cactataggg
      181 gatatcagct ggatggcaaa taatgatttt attttgactg atagtgacct gttcgttgca
      241 acaaattgat aagcaatgct ttcttataat gccaactttg tacaagaaag ctgggtcggc
      301 gcgcccaccc ttctggcagg gtgcaaacat cagcattttc atgccttgag gtaacttcag
      361 caccttttct aaaacttaat gagcttctta ataacaagtt atgaaaaacc ttgctaaatt
      421 acattttctt acaaatctac attacaggaa acaattgagt tgctttctaa gagactgtaa
      481 gacctgtctg ctgacttagg gacgctttta ggtggctgaa ttaggtggga aaagtgttag
      541 agctggtaat gagcagtggt ggttgggtgg ggtggcctgc agggtgagaa ctgaaggcag
      601 agtttctgat ctttatgcca agaccaggcc acaggagtat taagtgcctg gcttaggaat
      661 ttaaagaaag aaagaggttg attgttatat ttaaaaaact atttccgggc tctctttacc
      721 caggaagaaa aagctaaagg tcactacaga tacgtaggga atatgataaa gcaggtctga
      781 aaacacaatt tgattttgta caaatgatgt cgcttctcac aggcttcatt aaacagagaa
      841 cgtgtttgat gacagaaacg cataaaggac ggaaatgcgt gctgtaatat ttacttatgt
      901 ttaaaaatag ccagatggaa ccaactggtg ccaaatgaaa aatgaccagc gtaggctgtg
      961 tttcttgaca aaacaccttc agcacccatt gtccaaaagc tgaccaaagg tagtagatgg
     1021 gaatgtggct ccctttgaac taggcagaag tgaagcagat gccttgtata ttcaaatggt
     1081 tggcatgtaa atttctacgg gcagtcctat tttctggaac aatgtgtatt tgtaggaatt
     1141 tggacattgt gacttcaaat tttcttccct ttcattgagg cgggtttgtg gtcagctgat
     1201 tttttttctg gtgatgagat gttttgcgaa gtaccgtgtc agcctcctac ccctaccccc
     1261 gtccctcttc agcaaatcct ctgggatagt ggggagggtg aagggggcgg ccgcggagcc
     1321 tgcttttttg tacaaagttg gcattataaa aaagcattgc tcatcaattt gttgcaacga
     1381 acaggtcact atcagtcaaa ataaaatcat tatttggggc ccgagcttaa gactggccgt
     1441 cgttttacaa cgtcgtgact gggaaaacat ccatgctagc gttaacgcga gagtagggaa
     1501 ctgccaggca tcaaataaaa cgaaaggctc agtcggaaga ctgggccttt cgttttatct
     1561 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg
     1621 ttgtgaagca acggcccgga gggtggcggg caggacgccc gccataaact gccaggcatc
     1681 aaactaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa actcttcctg
     1741 gctagcggta cgcgtattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     1801 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     1861 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     1921 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     1981 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     2041 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     2101 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     2161 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     2221 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     2281 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2341 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2401 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2461 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     2521 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     2581 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     2641 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gacgcgtaac tcacgttaag
     2701 ggattttggt catgagcttg cgccgtcccg tcaagtcagc gtaatgctct gccagtgtta
     2761 caaccaatta accaattctg attagaaaaa ctcatcgagc atcaaatgaa actgcaattt
     2821 attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga
     2881 aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac
     2941 tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga
     3001 gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagtttat gcatttcttt
     3061 ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa
     3121 accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg
     3181 acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat
     3241 attttcacct gaatcaggat attcttctaa tacctggaat gctgtttttc cggggatcgc
     3301 agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg
     3361 cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct
     3421 acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca agcgatagat
     3481 tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc
     3541 catgttggaa tttaatcgcg gcct
//
