LOCUS       sequence-478758-        7148 bp    DNA     circular SYN 16-JUL-2025
DEFINITION  jGCaMP8s expressed under an oligodendrocyte-specific promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7148)
  TITLE     Fyhn unpublished plasmids
REFERENCE   2  (bases 1 to 7148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAAV MBP:jGCaMP8s
FEATURES             Location/Qualifiers
     source          1..7148
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     CDS             2314..2331
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     2613..2634
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2740..2761)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3001..3020)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    3592..4180
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3645..3665)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4063..4074)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4223..4344
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4234..4253)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4288..4307
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   4506..4635
                     /label=AAV2 ITR
     rep_origin      complement(4872..5460)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4952..4971)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5631..6422,6423..6491))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6254..6273
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6492..6596)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6622..7077)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6759..6780)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6971..6990
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatcg taccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      241 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      301 caagtgtatc atatggcggc cgctagagct ccttcctgct taggccgtgt ctgctctgtc
      361 agtctttcct ctggctctag tctgtccatc tgcagggata acctcccaag ggccctcttg
      421 ttggctgttc accttccgtc tgtcttcata gcctcctgat gagagagcgc gcacacacag
      481 gctacctgac tgatgtgttg ctgcactgtg gggtccagtg acacagctgt ggaggtcagg
      541 aacccagaat taagctgtct gggagccaca agtttcaaga gtccatgcta tggctcctac
      601 atgcaggtgg tgccagcagc attccttggt gtcctgtggc tgtggttgca tctctgactc
      661 tcctccacct atcacattcc ttctccagta ccaggcctct gtgtctcagt tttcctctct
      721 cacaagatcc ctagcttcag gattgcaccc acctccacta gccatgagcc cgtcctcact
      781 ggatcacgtc tgcaaagact ctatttccag ttaaagtcac agacacgggc atagagaggg
      841 ggataggata tgaaaatatc agttggggga ggggcattca attcttagct cacattttct
      901 tcatccaatt tcccccacta gaatgtaagc tttgagagaa aagggaccag atcttattcc
      961 tcaccgtggc tttaacactt agagaaaatg catcccctct aatcaataag tcatcgacag
     1021 tgggtagatg gaggaacggc agtgcgtagt aggatgcgtg caagcatagt ctcgtgcatg
     1081 ggtgcataga tcgctgggca ggtggacaag gtgggggtgg ataaagaagt gggtagatga
     1141 ttgatgttag gtaaatatca ctgggtggac agatgggtgg taggtggatg gatggttaga
     1201 atagtcagaa gagggatgga ttgataaggt gaacagatga taaatgggtg atagactgga
     1261 agggttgtca aaagaggata agggaagtgt gagctagccg tatttctaag gtcagtaata
     1321 gagttgggag aagaggttaa gttacatcca tttaaacctc acacgaagct gagagggaat
     1381 ggacttgctg ccgttggtga ggaaagcgtt gcatttcccg tgtgcttggt tgtgaagtgc
     1441 tcaggtccca catgaagcag tcaggttact gcggcttaca gaggagccag atccaaatgc
     1501 cccgagtaag cacgtccccg agccagaggc ctccagcgga atccgggaga gggattgctc
     1561 agtgccctgc ttccctggac tgtaagctgc agaaagatgt gggaagtcct gttctccact
     1621 gagaacacta aaagcacctt ttgtcaaacg accgcttcac atctggggct tgtgcactgg
     1681 tggcctttta aaccagagac aacccacaag atacctaacc tgcggggctc tctggtacag
     1741 tgagcaactc aggaaatgct ttggcttgat tgctgtgggc tctcaggcca tcgccctctg
     1801 gagtggttct tttaatgaga acctgaagat tggcccctga gccatgtata ccaagcaagc
     1861 tcaatccagg ttagctccct ctggttgggg caagctaacg tgctccttgg gccccgcgcg
     1921 taactgtgcg ttttatagga gacagctagt tcaagacccc aggaagaaag cggctttgtc
     1981 cccctctagg cctcgtacag gcccacattc atatctcatt gttgttgcag gggaggcaga
     2041 tgcgatccag aacaatggga cctcggctga ggacacggcg gtgacagact ccaagcacac
     2101 agcagaccca aagaataact ggcaaggcgc ccacccagct gacccaggga accgccccca
     2161 cttgatccgc ctcttttccc gagatgcccc gggaagggag gacaacacct tcaaagacag
     2221 gccctcagag tccgacgagc ttcagaccat ccaagaagac cccacagcag cttccggaag
     2281 ctcgaataat tctaggatcc acccgccacc atgcatcatc accatcatca cacgcgtcgc
     2341 aagaagacct tcaaggaggt ggccaccgcc gtgaagatca tcgccatgct gatgggcctg
     2401 aagatcaacg tctatatcaa ggccgacaag cagaagaacg gcatcaaggc gaacttccac
     2461 atccgccaca acatcgagga cggcggcgtg cagctcgcct accactacca gcagaacacc
     2521 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcgt ggagtccaaa
     2581 ctttcgaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     2641 gccgggatca ctctcggcat ggacgagctg tacaagggcg gtaccggagg gagcatggtg
     2701 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     2761 gtaaacggcc acaagttcag cgtgtccggc gagggtgagg gcgatgccac ctacggcaag
     2821 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     2881 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     2941 gacttcttca agtccgccat gcccgaaggc tacatccagg agcgcaccat cttcttcaag
     3001 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     3061 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     3121 gagtacaacc tgccggacca actgactgaa gagcagatcg cagaatacaa agaggctttc
     3181 tccctatttg acaaggacgg ggatgggaca ataacaacca aggagctggg gacggtgatg
     3241 cggtctctcg gacacaaccc cacagaagca gagctgcagg acatgatcaa tgaagtagat
     3301 gccgacggtg acggcacaat cgacttccct gagttcctga caatgatggc aagaaaaatg
     3361 aaatacaggg acacggaaga agaaattaga gaagcgttcg gtgtgtttga taaggatggc
     3421 aatggctaca tcagtgcagc agagcttcgc cacgtgatga caaaccttgg agagaagtta
     3481 acagatgaag aggttgatga aatgatcagg gaagcagaca tcgatgggga tggtcaggta
     3541 aactacgaag agtttgtaca aatgatgaca gcgaagtaaa agcttatcga taatcaacct
     3601 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     3661 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     3721 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     3781 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     3841 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     3901 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     3961 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     4021 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     4081 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     4141 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacccggg
     4201 cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga
     4261 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     4321 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     4381 cagggggaga tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc
     4441 gataaggatc ttcctagagc atggctacgt agataagtag catggcgggt taatcattaa
     4501 ctacaaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac
     4561 tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag
     4621 cgagcgagcg cgcagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     4681 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     4741 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     4801 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     4861 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     4921 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     4981 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     5041 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     5101 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     5161 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     5221 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     5281 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     5341 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     5401 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     5461 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     5521 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     5581 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     5641 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     5701 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     5761 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     5821 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     5881 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     5941 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     6001 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     6061 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     6121 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     6181 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     6241 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     6301 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     6361 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     6421 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     6481 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     6541 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     6601 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     6661 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     6721 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     6781 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     6841 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     6901 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     6961 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     7021 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     7081 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     7141 tacgccag
//
