LOCUS       sequence-478934-        6601 bp    DNA     circular SYN 17-JUL-2025
DEFINITION  Localization: Actin Related Protein, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6601)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-ARP2-N-14
FEATURES             Location/Qualifiers
     source          1..6601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     20..40
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1365..2042
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             2091..2768
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
     polyA_signal    2890..3011
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2927..2946)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2981..3000
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3018..3473)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3155..3176)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3367..3386
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3500..3604
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3606..3963
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3606..3797
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3629..3649)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3814..3949
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3998..4792
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3998..4792
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4052..4071)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4662..4681
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4980..4999)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5024..5071
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5400..5988
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5889..5908
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        6163..6466
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(6467..6601,1..69)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
       61 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggactca gatctcgaga
      121 ccgccatgga cagccagggc aggaaggtgg tggtgtgcga caacggcacc gggtttgtga
      181 agtgtggata tgcaggctct aactttccag aacacatctt cccagctttg gttggaagac
      241 ctattatcag atcaaccacc aaagtgggaa acattgaaat caagaataac aaaaagatgg
      301 atcttatggt tggtgatgag gcaagtgaat tacgatcaat gttagaagtt aactacccta
      361 tggaaaatgg catagtacga aattgggatg acatgaaaca cctgtgggac tacacatttg
      421 gaccagagaa acttaatata gataccagaa attgtaaaat cttactcaca gaacctccta
      481 tgaacccaac caaaaacaga gagaagattg tagaggtaat gtttgaaact taccagtttt
      541 ccggtgtata tgtagccatc caggcagttc tgactttgta cgctcaaggt ttattgactg
      601 gtgtagtggt agactctgga gatggtgtga ctcacatttg cccagtatat gaaggctttt
      661 ctctccctca tcttaccagg agactggata ttgctgggag ggatataact agatatctta
      721 tcaagctact tctgttgcga ggatacgcct tcaaccactc tgctgatttt gaaacggttc
      781 gcatgattaa agaaaaactg tgttacgtgg gatataatat tgagcaagag cagaaactgg
      841 ccttagaaac cacagtatta gttgaatctt atacactccc agatggacgt atcatcaaag
      901 ttgggggaga gagatttgaa gcaccagaag ctttatttca gcctcacttg atcaatgttg
      961 aaggagttgg tgttgctgaa ttgcttttta acacaattca ggcagctgac attgatacca
     1021 gatctgaatt ctacaaacac attgtgcttt ctggagggtc tactatgtat cctggcctgc
     1081 catcacggtt ggaacgagaa cttaaacagc tttacttaga acgagttttg aagggtgatg
     1141 tggaaaaact ttctaaattt aagatccgca ttgaagaccc accccgcaga aagcacatgg
     1201 tattcctggg tggtgcagtt ctagcggata tcatgaaaga caaagacaac ttttggatga
     1261 cccgacaaga gtaccaagaa aagggtgtcc gtgtgctaga gaaacttggt gtgactgttc
     1321 gagggggtgc gtcaggcggc agcggatctg gaccggtcgc caccatgagt gcgattaagc
     1381 cagacatgaa gatcaacctc cgtatggaag gcaacgtaaa cgggcaccac tttgtgatcg
     1441 acggagatgg tacaggcaag ccttttgagg gaaaacagag tatggatctt gaagtcaaag
     1501 agggcggacc tctgcctttt gcctttgata tcctgaccac tgcattccat tacggcaaca
     1561 gggtattcgc cgaatatcca gaccacatac aagactattt taagcagtcg tttcctaagg
     1621 ggtattcgtg ggaacgaagc ttgactttcg aagacggggg catttgcatt gccagaaacg
     1681 acataacaat ggaaggggac actttctata ataaagttcg atttcacggt gtaaactttc
     1741 ccgccaatgg tccagttatg cagaagaaga cgctgaaatg ggagccctcc actgagaaaa
     1801 tgtatgtgcg tgatggagtg ctgactggtg atattcgcat ggctttgttg cttgaaggaa
     1861 atgcccatta ccgatgtgac ttcagaacta cttacaaagc taaggagaag ggtgtcaagt
     1921 taccaggcta ccactttgtg gaccactgca ttgagatttt aagccatgac aaagattaca
     1981 acaaggttaa gctgtatgag catgctgttg ctcattctgg attgcctgac aatgccagac
     2041 gatccggtag cggtcacggt actggttcta ctggttctgg ttcttctgag agtgcgatta
     2101 agccagacat gaagatcaac ctccgtatgg aaggcaacgt aaacgggcac cactttgtga
     2161 tcgacggaga tggtacaggc aagccttttg agggaaaaca gagtatggat cttgaagtca
     2221 aagagggcgg acctctgcct tttgcctttg atatcctgac cactgcattc cattacggca
     2281 acagggtatt cgccgaatat ccagaccaca tacaagacta ttttaagcag tcgtttccta
     2341 aggggtattc gtgggaacga agcttgactt tcgaagacgg gggcatttgc attgccagaa
     2401 acgacataac aatggaaggg gacactttct ataataaagt tcgatttcac ggtgtaaact
     2461 ttcccgccaa tggtccagtt atgcagaaga agacgctgaa atgggagccc tccactgaga
     2521 aaatgtatgt gcgtgatgga gtgctgactg gtgatattcg catggctttg ttgcttgaag
     2581 gaaatgccca ttaccgatgt gacttcagaa ctacttacaa agctaaggag aagggtgtca
     2641 agttaccagg ctaccacttt gtggaccact gcattgagat tttaagccat gacaaagatt
     2701 acaacaaggt taagctgtat gagcatgctg ttgctcattc tggattgcct gacaatgcca
     2761 gacgataagc ggccgcgact ctagatcata atcagccata ccacatttgt agaggtttta
     2821 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     2881 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     2941 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     3001 aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     3061 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     3121 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     3181 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     3241 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     3301 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     3361 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     3421 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
     3481 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     3541 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     3601 gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     3661 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     3721 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     3781 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     3841 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     3901 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     3961 aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     4021 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     4081 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     4141 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
     4201 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     4261 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     4321 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     4381 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     4441 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     4501 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
     4561 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     4621 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     4681 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     4741 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     4801 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     4861 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     4921 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact
     4981 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
     5041 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
     5101 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
     5161 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
     5221 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat
     5281 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     5341 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     5401 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     5461 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     5521 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     5581 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     5641 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     5701 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     5761 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     5821 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     5881 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     5941 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6001 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     6061 ttatcccctg attctgtgga taaccgtatt accgccatgc attagttatt aatagtaatc
     6121 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     6181 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     6241 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     6301 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     6361 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     6421 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     6481 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     6541 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     6601 t
//
