LOCUS       sequence-478970-        8369 bp    DNA     circular SYN 04-AUG-2025
DEFINITION  Expresses Gal4-LATeNT* cargo in mammalian cells; used with 
            UAS-mCherry reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8369)
  AUTHORS   Roh H, Kim D, Kim B, Jeon Y, Kim Y, Jacko M, Xu F, Lin C, Won Um J, 
            Ting AY
  TITLE     Light-activated tetanus neurotoxin for conditional proteolysis and 
            inducible synaptic inhibition in vivo
  JOURNAL   bioRxiv 2025.01.27.635161; doi: 10.1101/2025.01.27.635161
REFERENCE   2  (bases 1 to 8369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.01.27.635161; doi: 10.1101/2025.01.27.635161"
COMMENT     Sequence Label: pcDNA3-Gal4-LATeNT(slow)
FEATURES             Location/Qualifiers
     source          1..8369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      901..910
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             913..939
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             940..1377
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="KLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKR
                     SPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKD
                     AVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             1378..1719
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     CDS             1999..2040
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        complement(3922..3940)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3923..3940)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3960..3977)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3966..4190
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4236..4664
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4323..4342)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4533..4554
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4673..4693)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4678..5007
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4858..4993
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4920..4939
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5074..5868
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5128..5147)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5738..5757
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6042..6163
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6079..6098)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6133..6152
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6212..6228)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6212..6228)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6225..6247)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6236..6252
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6260..6266,6267..6284,6285..6290))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6305..6326
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6443..6460)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6614..7202)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6694..6713)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7373..8164,8165..8233))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7996..8015
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8234..8338)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgcggcc
      901 gccaccatgg ggtatccata tgatgttcca gattatgcta agctactgtc ttctatcgaa
      961 caagcatgcg atatttgccg acttaaaaag ctcaagtgct ccaaagaaaa accgaagtgc
     1021 gccaagtgtc tgaagaacaa ctgggagtgt cgctactctc ccaaaaccaa aaggtctccg
     1081 ctgactaggg cacatctgac agaagtggaa tcaaggctag aaagactgga acagctattt
     1141 ctactgattt ttcctcgaga agaccttgac atgattttga aaatggattc tttacaggat
     1201 ataaaagcat tgttaacagg attatttgta caagataatg tgaataaaga tgccgtcaca
     1261 gatagattgg cttcagtgga gactgatatg cctctaacat tgagacagca tagaataagt
     1321 gcgacatcat catcggaaga gagtagtaac aaaggtcaaa gacagttgac tgtatcggcc
     1381 aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt cactaacagt
     1441 agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc acaaccaatt
     1501 gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag taaaattgat
     1561 gatggtaata attcaaaacc actgtcacct ggttggacgg accaaactgc gtataacgcg
     1621 tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata taactatcta
     1681 ttcgatgatg aagatacccc accaaaccca aaaaaagagt cggctaccgc tgccaccgtc
     1741 ccgcctgccg ccccggccgg cgagggtggc ccccctgcac ctcctccaaa ccttactagt
     1801 aacaggagac tgcagcagac ccaggcccag gtggatgagg tggtggacat catgagggtg
     1861 aatgtggaca aggtcctgga gcgggaccag aagttgtcgg agctggatga ccgtgcagat
     1921 gccctccagg caggggcctc ccagtttgaa acaagtgcag ccaagctcaa gcgcaaatac
     1981 tggtggaaaa acctcaaggg caagcccatc cccaaccccc tgctgggcct ggacagcacc
     2041 aagggctcgg gctcgacctc gggctcgggc accggtccga tcaccatcaa caacttccgt
     2101 tactccgacc cggttaacaa cgacaccatc atcatgatgg aaccgccgta ctgcaaaggt
     2161 ctagacatct actacagagc cttcaaaatc accgaccgta tctggatcgt tccggaacgt
     2221 tacgaatttg gtaccaaacc ggaagacttc aacccgccgt cctccctgat cgaaggtgct
     2281 tccgaatact acgacccgaa ctacctgcgt accgactccg acaaagaccg tttcctgcag
     2341 accatggtta aactgttcaa ccgtatcaaa aacaacgttg ctggtgaagc tctgctggac
     2401 aaaatcatca acgctatccc gtacctgggt aactcctact ccctgctgga caaattcgac
     2461 accaactcca actccgtttc cttcaacctg ctggaacagg atccgtccgg tgctaccacc
     2521 aaatccgcta tgctgaccaa cctgatcatc ttcggtccgg gtccggttct gaacaaaaac
     2581 gaagttcgtg gtatcgttct gcgtgttgac aacaaaaact acttcccgtg ccgtgacggt
     2641 ttcggttcca tcatgcagat ggctttctgc ccggaatacg taccgacctt cgacaacgtt
     2701 atcgaaaaca tcacctccct gaccatcggt aaatccaaat acttccagga cccggctctg
     2761 ctgctgatgc acgaactgat ccacgttctg cacggtctgt acggcatgca ggtttcctcc
     2821 cacgaaatca tcccgtccaa acaggaaatc tacatgcagc acacctaccc gatctccgct
     2881 gaagagctct tcaccttcgg tggtcaggac gctaacctga tctccatcga tatcaaaaac
     2941 gacctgtacg aaaaaaccct gaacgactac aaagctatcg ctaacaaact gtcccaggtt
     3001 acctcctgca acgacccgaa catcgacatc gactcctaca aacagatcta tcagcagaag
     3061 tatcagtttg ataaggattc caacggccag tatatagtga acgaggataa gtttcagata
     3121 ctgtataact ccataatgta tggcgggagc ggcagcgggg ggtccggtag cggggagttt
     3181 cgggcaacca cactggaacg gatcgagaaa agcttcctga ttactgatcc gagactgcct
     3241 gacaacccaa tcatttttgt gagcaattcc ttcctgcagc tgacagaata ttctcgggaa
     3301 gagatcctgg ggcgcaattg ccgttttctg cagggacccg agacagaccg tgccactgtt
     3361 cggaaaatca gagatgctat tgacaaccag actgaagtga ccgttcagct gatcaattat
     3421 accaagagcg gcaagaagtt ctggaacgtg ttccacctgc agccgatgcg cgattataag
     3481 ggcgacgtcc agtacttcat tggcgtgcag ctggatggca ccgaacgcct tcatggcgcc
     3541 gctgagcgtg aggcggtctg cctggtcaaa aagacagcct ttcagatttt tactgagata
     3601 gagctgggca agaagtttaa cataaagact cgcttaagct atttttccat gaaccacgat
     3661 cctgtgaaga tacctaacct gctggatgat actatatata acgatactga gggctttaac
     3721 atagagtcca aggatctgaa gtccgagtat aagggccaga acatgagggt gaacactaac
     3781 gcctttagga acgtcgacgg ttccggtctg gtttccaaac tgatcggtct gtgcaaaaaa
     3841 atcatcccgc cgaccaacat ccgtgaaaac ctgtacaacc gtaccgctta ataactcgag
     3901 catgcatcta gagggcccta ttctatagtg tcacctaaat gctagagctc gctgatcagc
     3961 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     4021 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     4081 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     4141 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     4201 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     4261 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     4321 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4381 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     4441 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4501 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4561 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     4621 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     4681 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4741 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     4801 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     4861 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4921 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     4981 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     5041 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     5101 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     5161 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     5221 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     5281 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     5341 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     5401 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     5461 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     5521 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     5581 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     5641 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     5701 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     5761 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     5821 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     5881 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     5941 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     6001 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     6061 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6121 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     6181 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     6241 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     6301 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     6361 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     6421 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     6481 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     6541 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     6601 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     6661 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6721 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6781 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     6841 aggtcgttcg ctccaagctg ggctgtgtgc acaaaccccc cgttcagccc gaccgctgcg
     6901 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6961 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7021 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     7081 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7141 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7201 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     7261 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     7321 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     7381 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     7441 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     7501 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     7561 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     7621 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     7681 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     7741 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     7801 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     7861 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     7921 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     7981 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     8041 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     8101 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     8161 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     8221 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     8281 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     8341 catttccccg aaaagtgcca cctgacgtc
//
