LOCUS       sequence-478980-        9395 bp    DNA     circular SYN 27-AUG-2025
DEFINITION  Expresses LATeNT-mCherry in yeast cytosol.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9395)
  AUTHORS   Roh H, Kim D, Kim B, Jeon Y, Kim Y, Jacko M, Xu F, Lin C, Won Um J, 
            Ting AY
  TITLE     Light-activated tetanus neurotoxin for conditional proteolysis and 
            inducible synaptic inhibition in vivo
  JOURNAL   bioRxiv 2025.01.27.635161; doi: 10.1101/2025.01.27.635161
REFERENCE   2  (bases 1 to 9395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 27, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.01.27.635161; doi: 10.1101/2025.01.27.635161"
COMMENT     Sequence Label: pyGAIP-LATeNT-mCherry
FEATURES             Location/Qualifiers
     source          1..9395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..504
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     546..564
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(602..624)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     724..743
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(1237..2331)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(2332..2739)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      complement(3040..3495)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3177..3198)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3389..3408
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3621..3643
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3635..3652
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3636..3652
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3662..3681
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3662..3680
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(3699..3946)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     3912..3930
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(3956..4663)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(4036..4059)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(4234..4253)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4503..4521
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      6487..6496
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6562..7003)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(6568..6591)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    6886..7003
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(7194..7214)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7194..7212)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7233..7249)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7233..7249)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7246..7268)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7257..7273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7281..7287,7288..7305,7306..7311))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7326..7347
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7464..7481)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7635..8223)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7715..7734)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8394..9185,9186..9254))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9017..9036
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9255..9359)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atcacgtgct ataaaaataa ttataattta aattttttaa tataaatata taaattaaaa
       61 atagaaagta aaaaaagaaa ttaaagaaaa aatagttttt gttttccgaa gatgtaaaag
      121 actctagggg gatcgccaac aaatactacc ttttatcttg ctcttcctgc tctcaggtat
      181 taatgccgaa ttgtttcatc ttgtctgtgt agaagaccac acacgaaaat cctgtgattt
      241 tacattttac ttatcgttaa tcgaatgtat atctatttaa tctgcttttc ttgtctaata
      301 aatatatatg taaagtacgc tttttgttga aattttttaa acctttgttt attttttttt
      361 cttcattccg taactcttct accttcttta tttactttct aaaatccaaa tacaaaacat
      421 aaaaataaat aaacacagag taaattccca aattattcca tcattaaaag atacgaggcg
      481 cgtgtaagtt acaggcaagc gatccgtcct aagaaaccat tattatcatg acattaacct
      541 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
      601 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
      661 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
      721 atgcggcatc agagcagatt gtactgagag tgcaccatat cgactacgtc gttaaggccg
      781 tttctgacag agtaaaattc ttgagggaac tttcaccatt atgggaaatg gttcaagaag
      841 gtattgactt aaactccatc aaatggtcag gtcattgagt gttttttatt tgttgtattt
      901 tttttttttt agagaaaatc ctccaatata taaattagga atcatagttt catgattttc
      961 tgttacacct aactttttgt gtggtgccct cctccttgtc aatattaatg ttaaagtgca
     1021 attctttttc cttatcacgt tgagccatta gtatcaattt gcttacctgt attcctttac
     1081 atcctccttt ttctccttct tgataaatgt atgtagattg cgtatatagt ttcgtctacc
     1141 ctatgaacat attccatttt gtaatttcgt gtcgtttcta ttatgaattt catttataaa
     1201 gtttatgtac aaatatcata aaaaaagaga atctttttaa gcaaggattt tcttaacttc
     1261 ttcggcgaca gcatcaccga cttcggtggt actgttggaa ccacctaaat caccagttct
     1321 gatacctgca tccaaaacct ttttaactgc atcttcaatg gccttacctt cttcaggcaa
     1381 gttcaatgac aatttcaaca tcattgcagc agacaagata gtggcgatag ggttgacctt
     1441 attctttggc aaatctggag cagaaccgtg gcatggttcg tacaaaccaa atgcggtgtt
     1501 cttgtctggc aaagaggcca aggacgcaga tggcaacaaa cccaaggaac ctgggataac
     1561 ggaggcttca tcggagatga tatcaccaaa catgttgctg gtgattataa taccatttag
     1621 gtgggttggg ttcttaacta ggatcatggc ggcagaatca atcaattgat gttgaacctt
     1681 caatgtaggg aattcgttct tgatggtttc ctccacagtt tttctccata atcttgaaga
     1741 ggccaaaaca ttagctttat ccaaggacca aataggcaat ggtggctcat gttgtagggc
     1801 catgaaagcg gccattcttg tgattctttg cacttctgga acggtgtatt gttcactatc
     1861 ccaagcgaca ccatcaccat cgtcttcctt tctcttacca aagtaaatac ctcccactaa
     1921 ttctctgaca acaacgaagt cagtaccttt agcaaattgt ggcttgattg gagataagtc
     1981 taaaagagag tcggatgcaa agttacatgg tcttaagttg gcgtacaatt gaagttcttt
     2041 acggattttt agtaaacctt gttcaggtct aacactaccg gtaccccatt taggaccacc
     2101 cacagcacct aacaaaacgg catcagcctt cttggaggct tccagcgcct catctggaag
     2161 tggaacacct gtagcatcga tagcagcacc accaattaaa tgattttcga aatcgaactt
     2221 gacattggaa cgaacatcag aaatagcttt aagaacctta atggcttcgg ctgtgatttc
     2281 ttgaccaacg tggtcacctg gcaaaacgac gatcttctta ggggcagaca ttagaatggt
     2341 atatccttga aatatatata tatatattgc tgaaatgtaa aaggtaagaa aagttagaaa
     2401 gtaagacgat tgctaaccac ctattggaaa aaacaatagg tccttaaata atattgtcaa
     2461 cttcaagtat tgtgatgcaa gcatttagtc atgaacgctt ctctattcta tatgaaaagc
     2521 cggttccggc gctctcacct ttcctttttc tcccaatttt tcagttgaaa aaggtatatg
     2581 cgtcaggcga cctctgaaat taacaaaaaa tttccagtca tcgaatttga ttctgtgcga
     2641 tagcgcccct gtgtgttctc gttatgttga ggaaaaaaat aatggttgct aagagattcg
     2701 aactcttgca tcttacgata cctgagtatt cccacagtta actgcggtca agatatttct
     2761 tgaatcaggc gccttagacc gctcggccaa acaaccaatt acttgttgag aaatagagta
     2821 taattatcct ataaatataa cgtttttgaa cacacatgaa caaggaagta caggacaatt
     2881 gattttgaag agaatgtgga ttttgatgta attgttggga ttccattttt aataaggcaa
     2941 taatattagg tatgtagata tactagaagt tctcctcgac cggtcgatat gcggtgtgaa
     3001 ataccgcaca gatgcgtaag gagaaaatac cgcatcagga aattgtaagc gttaatattt
     3061 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     3121 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     3181 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     3241 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     3301 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     3361 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     3421 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     3481 cgctacaggg cgcgtccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     3541 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     3601 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc
     3661 gtaatacgac tcactatagg gcgaattggg taccggccgc aaattaaagc cttcgagcgt
     3721 cccaaaacct tctcaagcaa ggttttcagt ataatgttac atgcgtacac gcgtctgtac
     3781 agaaaaaaaa gaaaaatttg aaatataaat aacgttctta atactaacat aactataaaa
     3841 aaataaatag ggacctagac ttcaggttgt ctaactcctt ccttttcggt tagagcggat
     3901 gtggggggag ggcgtgaatg taagcgtgac ataactaatt acatgactcg agctattact
     3961 tgtacagctc gtccatgccg ccggtggagt ggcggccctc ggcgcgctcg tactgttcca
     4021 cgatggtgta gtcctcgttg tgggaggtga tgtccagctt gatgttgacg ttgtaggcgc
     4081 cgggcagctg cacgggcttc ttggccttgt aggtggtctt gacctcggcg tcgtagtggc
     4141 cgccgtcctt cagcttcagc ctctgcttga tctcgccctt cagggcgccg tcctcggggt
     4201 acatccgctc ggaggaggcc tcccagccca tggtcttctt ctgcattacg gggccgtcgg
     4261 aggggaagtt ggtgccgcgc agcttcacct tgtagatgaa ctcgccgtcc tgcagggagg
     4321 agtcctgggt cacggtcacc acgccgccgt cctcgaagtt catcacgcgc tcccacttga
     4381 agccctcggg gaaggacagc ttcaagtagt cggggatgtc ggcggggtgc ttcacgtagg
     4441 ccttggagcc gtacatgaac tgaggggaca ggatgtccca ggcgaagggc agggggccgc
     4501 ccttggtcac cttcagcttg gcggtctggg tgccctcgta ggggcggccc tcgccctcgc
     4561 cctcgatctc gaactcgtgg ccgttcacgg agccctccat gtgcaccttg aagcgcatga
     4621 actccttgat gatggccatg ttgtcctcct cgcccttgct cacagcggta cggttgtaca
     4681 ggttttcacg gatgttggtc ggcgggatga tttttttgca cagaccgatc agtttggaaa
     4741 ccagaccgga accgtcgacg ttcctaaagg cgttagtgtt caccctcatg ttctggccct
     4801 tatactcgga cttcagatcc ttggactcta tgttaaagcc ctcagtatcg ttatatatag
     4861 tatcatccag caggttaggt atcttcacag gatcgtggtt catggaaaaa tagcttaagc
     4921 gagtctttat gttaaacttc ttgcccagct ctatctcagt aaaaatctga aaggctgtct
     4981 ttttgaccag gcagaccgcc tcacgctcag cggcgccatg aaggcgttcg gtgccatcca
     5041 gctgcacgcc aatgaagtac tggacgtcgc ccttataatc gcgcatcggc tgcaggtgga
     5101 acacgttcca gaacttcttg ccgctcttgg tataattgat cagctgaacg gtcacttcag
     5161 tctggttgtc aatagcatct ctgattttcc gaacagtggc acggtctgtc tcgggtccct
     5221 gcagaaaacg gcaattgcgc cccaggatct cttcccgaga atattctgtc agctgcagga
     5281 aggaattgct cacaaaaatg attgggttgt caggcagtct cggatcagta atcacgaagc
     5341 ttttctcgat ccgttccagt gtggttgccc gaaactcccc gctaccggac cccccgctgc
     5401 cgctcccgcc atacattatg gagttataca gtatctgaaa cttatcctcg ttcactatat
     5461 actggccgtt ggaatcctta tcaaactgat acttctgctg atagatctgt ttgtaggagt
     5521 cgatgtcgat gttcgggtcg ttgcaggagg taacctggga cagtttgtta gcgatagctt
     5581 tgtagtcgtt cagggttttt tcgtacaggt cgtttttgat atcgatggag atcaggttag
     5641 cgtcctgacc accgaaggtg aagagctctt cagcggagat cgggtaggtg tgctgcatgt
     5701 agatttcctg tttggacggg atgatttcgt gggaggaaac ctgcatgccg tacagaccgt
     5761 gcagaacgtg gatcagttcg tgcatcagca gcagagccgg gtcctggaag tatttggatt
     5821 taccgatggt cagggaggtg atgttttcga taacgttgtc gaaggtcggt acgtattccg
     5881 ggcagaaagc catctgcatg atggaaccga aaccgtcacg gcacgggaag tagtttttgt
     5941 tgtcaacacg cagaacgata ccacgaactt cgtttttgtt cagaaccgga cccggaccga
     6001 agatgatcag gttggtcagc atagcggatt tggtggtagc accggacgga tcctgttcca
     6061 gcaggttgaa ggaaacggag ttggagttgg tgtcgaattt gtccagcagg gagtaggagt
     6121 tacccaggta cgggatagcg ttgatgattt tgtccagcag agcttcacca gcaacgttgt
     6181 ttttgatacg gttgaacagt ttaaccatgg tctgcaggaa acggtctttg tcggagtcgg
     6241 tacgcaggta gttcgggtcg tagtattcgg aagcaccttc gatcagggag gacggcgggt
     6301 tgaagtcttc cggtttggta ccaaattcgt aacgttccgg aacgatccag atacggtcgg
     6361 tgattttgaa ggctctgtag tagatgtcta gacctttgca gtacggcggt tccatcatga
     6421 tgatggtgtc gttgttaacc gggtcggagt aacggaagtt gttgatggtg atcgggttaa
     6481 cgctagccat ggtggcgacc ggtggatccc gagccatctt aattgaaaat gtatgaagta
     6541 gaattcgatc cggggttttt tctccttgac gttaaagtat agaggtatat taacaatttt
     6601 ttgttgatac ttttattaca tttgaataag aagtaataca aaccgaaaat gttgaaagta
     6661 ttagttaaag tggttatgca gtttttgcat ttatatatct gttaatagat caaaaatcat
     6721 cgcttcgctg attaattacc ccagaaataa ggctaaaaaa ctaatcgcat tatcatccta
     6781 tggttgttaa tttgattcgt tcatttgaag gtttgtgggg ccaggttact gccaattttt
     6841 cctcttcata accataaaag ctagtattgt agaatcttta ttgttcggag cagtgcggcg
     6901 cgaggcacat ctgcgtttca ggaacgcgac cggtgaagac gaggacgcac ggaggagagt
     6961 cttccttcgg agggctgtca cccgctcggc ggcttctaat ccgtacttca atatagcaat
     7021 gagcagttaa gcgtattact gaaagttcca aagagaaggt ttttttaggc taagataatg
     7081 gggctcttta catttccaca acatataagt aagattagat atggatatgt atatggtggt
     7141 aatgccatgt aatatgatta ttaaacttct ttgcggagct ccagcttttg ttccctttag
     7201 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     7261 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     7321 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     7381 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     7441 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     7501 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     7561 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7621 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7681 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7741 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7801 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7861 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7921 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7981 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     8041 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     8101 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     8161 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     8221 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     8281 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     8341 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     8401 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     8461 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     8521 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     8581 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     8641 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     8701 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     8761 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     8821 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     8881 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     8941 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     9001 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     9061 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     9121 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     9181 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     9241 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     9301 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     9361 acatttcccc gaaaagtgcc acctgggtcc ttttc
//
