LOCUS       sequence-478988-        9270 bp    DNA     circular SYN 17-JUL-2025
DEFINITION  Fluorescent reporter for single ribosome tracking.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9270)
  AUTHORS   Katz ZB, English BP, Lionnet T, Yoon YJ, Monnier N, Ovryn B, Bathe 
            M, Singer RH
  TITLE     Mapping translation 'hot-spots' in live cells by tracking single 
            molecules of mRNA and ribosomes.
  JOURNAL   Elife. 2016 Jan 13;5. pii: e10415. doi: 10.7554/eLife.10415.
  PUBMED    26760529
REFERENCE   2  (bases 1 to 9270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-01-13"; volume: "5. pii: e10415. doi"; pages: " 
            10.7554/eLife.10415"
COMMENT     Sequence Label: p323-L10A-PATagRFP
FEATURES             Location/Qualifiers
     source          1..9270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(132..200,201..992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(350..369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1652..1671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1905..1922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2075..2080,2081..2098,2099..2105)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2118..2140
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2137..2153
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2172..2192
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        2293..2672
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2673..2876
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2827..2847
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2891..3071
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3118..3243
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3736..3969
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4154..4198
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4465..4582
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4639..5850
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5687..5706
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             6609..7301
                     /codon_start=1
                     /product="photoactivatable variant of the monomeric red 
                     fluorescent protein TagRFP (Subach et al., 2010)"
                     /label=PA-TagRFP
                     /note="mammalian codon-optimized"
                     /translation="ELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKVVE
                     GGPLPFAFDILATSFMYGSSTFINHTQGIPDFWKQSFPEGFTWERVTTYEDGGVLTATQ
                     DTSLQDGCLIYNVKIRGVNFPSNGPVMKKKTLGWEPSTEKLKPADGGLEGRVDMALKLV
                     GGGHLICNFKTTYRSKKPAKNLKMPGVYYVDRRLEIIKEADKETYWEQHEVAVARYSDL
                     PSKLGHKLN"
     misc_feature    7333..7921
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7386..7406)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7804..7815)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8008..8241
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8313..8434
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8350..8369)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8404..8423
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8474..8609
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8629..8648)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8630..8648)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8658..8675)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8658..8674)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8667..8689)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8816..9244
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8903..8922)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9113..9134
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttggc
     2221 cattgcatac gttgtatcca tatcataata tgtacattta tattggctca tgtccaacat
     2281 taccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     2341 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     2401 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     2461 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     2521 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     2581 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     2641 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     2701 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     2761 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     2821 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt
     2881 tagtgaaccg gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     2941 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     3001 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     3061 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     3121 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     3181 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     3241 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     3301 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     3361 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     3421 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     3481 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     3541 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     3601 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     3661 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     3721 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     3781 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     3841 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     3901 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     3961 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     4021 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     4081 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     4141 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     4201 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     4261 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     4321 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     4381 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
     4441 cggatctcga cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc
     4501 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     4561 aattacaaaa attcaaaatt ttatcgatca cgagactagc ctcgagaagc ttgattaacc
     4621 cgtgtcggct ccagatctgg cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc
     4681 tcctcacggc gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct gatccttccg
     4741 cccggacgct caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc
     4801 agcagaagga cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag
     4861 agagcggaac aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt
     4921 ggggcggtga acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt
     4981 cgcagccggg atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga
     5041 gtagcgggct gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga
     5101 agcgtgtgga gagaccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac
     5161 tgggggttgg ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc
     5221 ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg cggcaagaac
     5281 ccaaggtctt gaggccttcg ctaatgcggg aaagctctta ttcgggtgag atgggctggg
     5341 gcaccatctg gggaccctga cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc
     5401 tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt gcacccgtac ctttgggagc
     5461 gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc agggtggggc
     5521 cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta
     5581 gggtaggctc tcctgaatcg acaggcgccg gacctctggt gaggggaggg ataagtgagg
     5641 cgtcagtttc tttggtcggt tttatgtacc tatcttctta agtagctgaa gctccggttt
     5701 tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa
     5761 atgtaatcat ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa
     5821 ttctggccgt ttttggcttt tttgttagac gaagcttggg ctgcaggtcg agatccaccg
     5881 gtgctggcta gcgtttaaac gggccctcta gagccaccat gagcagcaaa gtttcacgcg
     5941 acaccctcta cgaggcggtg cgggaagtcc tgcacgggaa ccagcgtaag cgccgcaagt
     6001 ttctggagac ggtggagctg cagatcagcc tgaagaacta cgaccctcag aaggacaaac
     6061 gcttctcggg caccgtcagg cttaagtcca cccctcgccc caagttctcc gtgtgcgtcc
     6121 tgggggacca gcagcactgt gatgaagcca aggccgtgga tatcccccac atggacatcg
     6181 aggcgctcaa gaagcttaac aagaacaaga agttggtcaa aaagctggcc aagaagtatg
     6241 atgccttttt ggcctccgag tctctgatca agcaaatccc acgtatcctg ggcccaggcc
     6301 taaacaaggc gggcaagttc ccctccctgc tgacacacaa tgaaaacatg gtggccaaag
     6361 tggatgaggt gaaatccaca atcaagttcc agatgaagaa ggtgctgtgt ctggccgttg
     6421 ccgtcggcca cgtgaagatg accgatgacg agctagtcta caacatccac ctggctgtca
     6481 acttcttggt gtccttactc aagaaaaact ggcagaacgt ccgggctctg tacatcaaga
     6541 gcaccatggg caagccccag cgtctgtatg gatccggagg aggaggagga ggagtgagca
     6601 agggcgagga gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtga
     6661 acaaccacca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga
     6721 ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta
     6781 ccagcttcat gtacggcagc agcaccttca tcaaccacac ccagggcatc cccgacttct
     6841 ggaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg
     6901 gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca
     6961 agatcagagg ggtgaacttc ccatccaacg gccctgtgat gaagaagaaa acactcggct
     7021 gggaaccgag caccgagaaa ctgaaacccg ctgacggcgg cctggaaggc agggtcgaca
     7081 tggccctgaa gctcgtgggc gggggccacc tgatctgcaa cttcaagacc acatacagat
     7141 ccaagaaacc cgctaagaac ctcaagatgc ccggcgtcta ctatgtggac cgcagactgg
     7201 aaatcatcaa ggaggccgac aaagagacct actgggagca gcacgaggtg gctgtggcca
     7261 gatactccga cctccctagc aaactggggc acaaacttaa tggcatggac gagctgtaca
     7321 agtaaactcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     7381 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     7441 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     7501 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     7561 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     7621 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     7681 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     7741 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     7801 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     7861 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     7921 cctggaattc gagctcggta cctttaagac caatgactta caaggcagct gtagatctta
     7981 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     8041 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     8101 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     8161 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     8221 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     8281 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     8341 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     8401 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     8461 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     8521 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     8581 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     8641 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     8701 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     8761 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     8821 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     8881 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     8941 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     9001 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     9061 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     9121 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     9181 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     9241 aattttaaca aaatattaac gcttacaatt
//
