LOCUS       sequence-478996-        1104 bp    DNA     linear   UNA 08-AUG-2025
DEFINITION  Construct for labelling Golgi apparatus in mammalian cells using 
            mScarlet3-H-Giantin fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1104)
  TITLE     mScarlet3-H plasmids
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 8, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pmScarlet3-H-Giantin_C1
FEATURES             Location/Qualifiers
     source          1..1104
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..687
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent 
                     protein with fast and complete maturation (Gadella et al., 
                     2023)"
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
                     VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKH
                     ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     primer_bind     399..418
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atggatagca ccgaggcagt gatcaaggag ttcatgcggt tcaaggtgca catggagggc
       61 tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
      121 acccagaccg ccaagctgag ggtgaccaag ggtggccccc tgcccttctc ctgggacatc
      181 ctgtcccctc agttcatgta cggctccagg gccttcacga agcaccccgc cgacatcccc
      241 gactactgga agcagtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
      301 gacggcggcg ccgtgtccgt ggcccaggac acctccctgg aggacggcac cctgatctac
      361 aaggtgaagc tccgcggcac caacttccct cctgacggcc ccgtaatgca gaagaagaca
      421 atgggctggg aagcatccac cgagcggttg taccccgagg acgtcgtgct gaagggcgac
      481 attaagcacg ccctgcgcct gaaggacggc ggccgctacc tggcggactt caagaccacc
      541 tacagggcca agaagcccgt gcagatgccc ggcgccttca acatcgaccg caagttggac
      601 atcacatccc acaacgagga ctacaccgtg gtggaacagt acgaacgctc cgtggcccgc
      661 cactccaccg gcggctccgg tggctccctg tacaagtccg gactcagatc tcgaggagaa
      721 ccgcagcaaa gcttttctga agctcagcag cagctatgca acaccagaca ggaagtgaat
      781 gaattaagga agctgctgga agaagaacga gaccaaagag tggctgctga gaatgctctc
      841 tctgtggccg aggagcagat cagacggtta gagcacagtg aatgggactc ttcccggact
      901 cctatcattg gctcctgtgg cactcaggag caggcactgt taatagatct tacaagcaac
      961 agttgtcgaa ggacccggag tggcgttgga tggaagcgag tcctgcgttc actctgtcat
     1021 tcacggaccc gagtgccact tctagcagcc atctactttc taatgattca tgtcctgctc
     1081 attctgtgtt ttacgggcca tcta
//
