LOCUS       sequence-479000-       14501 bp    DNA     circular SYN 18-JUL-2025
DEFINITION  Transient expression of recombinant proteins in plants. The plasmid 
            contains gfp, which is replaced by the gene of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14501)
  AUTHORS   Mardanova ES, Blokhina EA, Tsybalova LM, Peyret H, Lomonossoff GP, 
            Ravin NV
  TITLE     Efficient Transient Expression of Recombinant Proteins in Plants by 
            the Novel pEff Vector Based on the Genome of Potato Virus X.
  JOURNAL   Front Plant Sci. 2017 Feb 28;8:247. doi: 10.3389/fpls.2017.00247. 
            eCollection 2017.
  PUBMED    28293244
REFERENCE   2  (bases 1 to 14501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 18, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2017-02-28"; volume: "8:247. doi"; pages: " 
            10.3389/fpls.2017.00247. eCollection 2017"
COMMENT     Sequence Label: pEff
FEATURES             Location/Qualifiers
     source          1..14501
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     regulatory      1052..1061
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4925..4946)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5186..5205)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5607..5628
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      5871..6123
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        6212..6557
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      7249..7501
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        7657..7836
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        7689..7872
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             7893..8687
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7947..7966)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8557..8576
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      9073..9325
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    9416..9440
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      9808..10438
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(10528..11676)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(11975..12769)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     complement(13359..13376)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(13610..13629)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    14061..14085
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     14224..14243
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14368..14390
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14382..14399
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14383..14399
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggagaaaac taaaccatac
      361 accaccaaca caaccaaacc caccacgccc aattgttaca cacccgcttg aaaaagaaag
      421 tttaacaaat ggccaaggtg cgcgaggttt accaatcttt tacagactcc accacaaaaa
      481 ctctcatcca agatgaggct tatagaaaca ttcgccccat catggaaaaa cacaaactag
      541 ctaaccctta cgctcaaacg gttgaagcgg ctaatgatct agaggggttc ggcatagcca
      601 ccaatcccta tagcattgaa ttgcatacac atgcagccgc taagaccata gagaataaac
      661 ttctagaggt gcttggttcc atcctaccac aagaacctgt tacatttatg tttcttaaac
      721 ccagaaagct aaactacatg agaagaaacc cgcggatcaa ggacattttc caaaatgttg
      781 ccattgaacc aagagacgta gccaggtacc ccaaggaaac aataattgac aaactcacag
      841 agatcacaac ggaaacagca tacattagtg acactctgca cttcttggat ccgagctaca
      901 tagtggagac attccaaaac tgcccaaaat tgcaaacatt gtatgcgacc ttagttctcc
      961 ccgttgaggc agcctttaaa atggaaagca ctcacccgaa catatacagc ctcaaatact
     1021 tcggagatgg tttccagtat ataccaggca accatggtgg cggggcatac catcatgaat
     1081 tcgctcatct acaatggctc aaagtgggaa agatcaagtg gagggacccc aaggatagct
     1141 ttctcggaca tctcaattac acgactgagc aggttgagat gcacacagtg acagtacagt
     1201 tgcaggaatc gttcgcggca aaccacttgt actgcatcag gagaggagac ttgctcacac
     1261 cggaggtgcg cactttcggc caacctgaca ggtacgtgat tccaccacag atcttcctcc
     1321 caaaagttca caactgcaag aagccgattc tcaagaaaac tatgatgcag ctcttcttgt
     1381 atgttaggac agtcaaggtc gcaaaaaatt gtgacatttt tgccaaagtc agacaattaa
     1441 ttaaatcatc tgacttggac aaatactctg ctgtggaact ggtttactta gtaagctaca
     1501 tggagttcct tgccgattta caagctacca cctgcttctc agacacactt tctggtggct
     1561 tgctaacaaa gacccttgca ccggtgaggg cttggataca agagaaaaag atgcagctgt
     1621 ttggtcttga ggactacgcg aagttagtca aagcagttga tttccacccg gtggattttt
     1681 ctttcaaagt ggaaacttgg gacttcagat tccacccctt gcaagcgtgg aaagccttcc
     1741 gaccaaggga agtgtcggat gtagaggaaa tggaaagttt gttctcagat ggggacttgc
     1801 ttgattgctt cacaagaatg ccagcttatg cggtaaacgc agaggaagat ttagctgcaa
     1861 tcaggaaaac gcccgagatg gatgtcggtc aagaagttaa agaacctgca ggagacagaa
     1921 atcaatactc aaaccctgca gaaactttcc tcaacaagct ccacaggaaa cacagtaggg
     1981 aggtgaaaca ccaggccgca aagaaagcta aacgcctagc tgaaatccag gagtcaatga
     2041 gagctgaagg tgatgccgaa ccaaatgaaa taagcggggg catgggggca atacccagca
     2101 acgccgaact tcctggcacg aatgatgcca gacaagaact cacactccca accactaaac
     2161 ctgtccctgc aaggtgggaa gatgcttcat tcacagattc tagtgtggaa gaggagcagg
     2221 ttaaacccct tggaaaagaa accgttgaaa cagcgacgca acaagtcatc gaaggacttc
     2281 cttggaaaca ctggattcct caattaaatg ctgttggatt caaggcgctg gaaattcaga
     2341 gggataggag tggaacaatg atcatgccca tcacagaaat ggtctccggg ctggaaaaag
     2401 aggacttccc tgaaggaact ccaaaagagt tggcacgaga attgttcgct atgaacagaa
     2461 gccctgccac catccctttg gacctgctta gagccagaga ctacggcagt gatgtaaaga
     2521 acaagagaat tggtgccatc acaaagacac aggcaacgag ttggggcgaa tacttgacag
     2581 gaaagataga aagcttgact gagaggaaag ttgcgacttg tgtcattcat ggagctggag
     2641 gttctggaaa aagtcatgcc atccagaagg cattgagaga aattggcaag ggctcggaca
     2701 tcactgtagt cctgccgacc aatgaactgc ggctagattg gagtaagaaa gtgcctaaca
     2761 ctgagcccta tatgttcaag acctatgaaa aggcgttaat tgggggaaca ggcagcatag
     2821 tcatctttga cgattactca aaacttcctc ccggttacat agaagcctta gtctgtttct
     2881 actctaaaat caagctaatc attctaacag gagatagcag acaaagcgtc taccatgaaa
     2941 ctgctgagga cgcctccatc aggcatttgg gaccagcaac agagtacttc tcaaaatact
     3001 gccgatacta tctcaatgcc acacaccgca acaagaaaga tcttgcgaac atgcttggtg
     3061 tctacagtga gagaacggga gtcaccgaaa tcagcatgag cgccgagttc ttagaaggaa
     3121 tcccaacttt ggtaccctcg gatgagaaga gaaagctgta catgggcacc gggaggaatg
     3181 acacgttcac atacgctgga tgccaggggc taactaagcc gaaggtacaa atagtgttgg
     3241 accacaacac ccaagtgtgt agcgcgaatg tgatgtacac ggcactttct agagccaccg
     3301 ataggattca cttcgtgaac acaagtgcaa attcctcggc cttctgggaa aagttggaca
     3361 gcacccctta cctcaagact ttcctatcag tggtgagaga acaagcactc agggagtacg
     3421 agccggcaga ggcagagcca attcaagagc ctgagcccca gacacacatg tgtgtcgaga
     3481 atgaggagtc cgtgctagaa gagtacaaag aggaactctt ggaaaagttt gacagagaga
     3541 tccactctga atcccatggt cattcaaact gtgtccaaac tgaagacaca accattcagt
     3601 tgttttcgca tcaacaagca aaagatgaga ccctcctctg ggcgactata gatgcgcggc
     3661 tcaagaccag caatcaagag acaaacttcc gagaattcct gagcaagaag gacattgggg
     3721 acgttctgtt tttaaactac caaaaagcta tgggtttacc caaagagcgt attccttttt
     3781 cccaagaggt ctgggaagct tgtgcccacg aagtacaaag caagtacctc agcaagtcaa
     3841 agtgcaactt gatcaatggg actgtgagac agagcccaga cttcgatgaa aataagatta
     3901 tggtattcct caagtcgcag tgggtcacaa aggtggaaaa actaggtcta cccaagatta
     3961 agccaggtca aaccatagca gccttttacc agcagactgt gatgcttttt ggaactatgg
     4021 ctaggtacat gcgatggttc agacaggctt tccagccaaa agaagtcttc ataaactgtg
     4081 agaccacgcc agatgacatg tctgcatggg ccttgaacaa ctggaatttc agcagaccta
     4141 gcttggctaa tgactacaca gctttcgacc agtctcagga tggagccatg ttgcaatttg
     4201 aggtgctcaa agccaaacac cactgcatac cagaggaaat cattcaggca tacatagata
     4261 ttaagactaa tgcacagatt ttcctaggca cgttatcaat tatgcgcctg actggtgaag
     4321 gtcccacttt tgatgcaaac actgagtgca acatagctta cacccataca aagtttgaca
     4381 tcccagccgg aactgctcaa gtttatgcag gagacgactc cgcactggac tgtgttccag
     4441 aagtgaagca tagtttccac aggcttgagg acaaattact cctaaagtca aagcctgtaa
     4501 tcacgcagca aaagaagggc agttggcctg agttttgtgg ttggctgatc acaccaaaag
     4561 gggtgatgaa agacccaatt aagctccatg ttagcttaaa attggctgaa gctaagggtg
     4621 aactcaagaa atgtcaagat tcctatgaaa ttgatctgag ttatgcctat gaccacaagg
     4681 actctctgca tgacttgttc gatgagaaac agtgtcaggc acacacactc acttgcagaa
     4741 cactaatcaa gtcagggaga ggcactgtct cactttcccg cctcagaaac tttctttaac
     4801 cgttaagtta ccttagagat ttgaataagt ctagattttt atttttaatt ttctttcaaa
     4861 tacttccatc aggcgcgcca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     4921 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     4981 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     5041 gcccgtgccc tggcccaccc tcgtgaccac cttcagctac ggcgtgcagt gcttcagccg
     5101 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     5161 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtatg
     5221 tagtgttttt ggataactga gtttgcctat gattttgtat ttactgagat gtttgtcctc
     5281 tttctgcagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     5341 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     5401 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     5461 cacaacaccg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     5521 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     5581 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     5641 atcactcacg gcatggacga gctgtacaag taacccgggt gtacaagtaa tctaggatac
     5701 cgtcgaggtc gtatcactgg aacaacaacc gctgaggctg ttgtcactct accaccacca
     5761 taactacgtc tacataaccg acgcctaccc cagtttcata gtattttctg gtttgattgt
     5821 atgaataata taaataaaaa aaaaaactag tgggtaccgc gaatttcccc gatcgttcaa
     5881 acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca
     5941 tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat
     6001 ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa
     6061 acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag
     6121 atcgtatacc tagggtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
     6181 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
     6241 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
     6301 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
     6361 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     6421 aagacggtcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     6481 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     6541 ttcatttgga gagaacacgg gggactctag aggatccgaa atgcgggtga tagtatcacc
     6601 atacgaagct gaagacattt taaaaagatc aacagacatg ctacgaaaca ttgatagcgg
     6661 tgttttgtct acaaaggaat gcattaaggc tttcagcacc atcactagag acctgcactg
     6721 tgcaaaggca tcttaccaat ggggagttga cacaggtttg tatcaacgta attgcgcaga
     6781 aaagcacctt attgatacag ttgaaagtaa cataaggttg gcacagccgc ttgttaggga
     6841 gaaggtagca gtgcattttt gcaaggatga gcctaaagaa cttgttgcct tcataactag
     6901 aaaatatgtc gaattgactg gagtaggtgt tagggaagct gttaaaaggg agatgcggag
     6961 tctcactaag acagttctca ataagatgtc cctcgaaatg gcattctata tgtcacctag
     7021 agcttggaag aacgcagaat ggctggaatt gaaatttagt cccgtaaaga ttttcagaga
     7081 tttgcttctc gatgttgaaa ctctcaatga actctgcgca gaggatgacg ttcatgtcga
     7141 taaagttaat gaaaacggtg atgagaacca tgatctcgaa cttcaggatg agtgctgaat
     7201 ttaaattgta caagtaaagc ggccctgcag gtcgactcga atttccccga tcgttcaaac
     7261 atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata
     7321 taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt
     7381 atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac
     7441 aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat
     7501 ctacgtaccc cgcgccagct tggcgtaatc atggtcatag ctgttgcgat cgctctgcag
     7561 ataacgcgtg gccggccatg agcggagaat taagggagtc acgttatgac ccccgccgat
     7621 gacgcgggac aagccgtttt acgtttggaa ctgacagaac cgcaacgttg aaggagccac
     7681 tcagccgcgg gtttctggag tttaatgagc taagcacata cgtcagaaac cattattgcg
     7741 cgttcaaaag tcgcctaagg tcactatcag ctagcaaata tttcttgtca aaaatgctcc
     7801 actgacgttc cataaattcc cctcggtatc caattagagt ctcatattca ctctcaatcc
     7861 aaataatctg caccggatct ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     7921 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     7981 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     8041 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     8101 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     8161 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     8221 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     8281 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     8341 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     8401 tgttcgccag gctcaaggcg cgcatgcccg acggcgatga tctcgtcgtg acccatggcg
     8461 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     8521 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     8581 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     8641 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     8701 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     8761 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     8821 ccagcgcggg gatctcatgc tggagttctt cgcccacggg atctctgcgg aacaggcggt
     8881 cgaaggtgcc gatatcatta cgacagcaac ggccgacaag cacaacgcca cgatcctgag
     8941 cgacaatatg atcgcggcgt ccacatcaac ggcgtcggcg gcgactgccc aggcaagacc
     9001 gagatgcacc gcgatatctt gctgcgttcg gatattttcg tggagttccc gccacagacc
     9061 cggatgatcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt
     9121 gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt
     9181 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
     9241 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc
     9301 gcggtgtcat ctatgttact agatcggggg ccggccctca ctggtgaaaa gaaaaaccac
     9361 cccagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgttta
     9421 caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag ctcggcacaa
     9481 aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg agagccgttg
     9541 taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc
     9601 cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag
     9661 cgttgctgcc tgtgatcaaa tatcatctcc ctcgcagaga tccgaattat cagccttctt
     9721 attcatttct cgcttaaccg tgacagagta gacaggctgt ctcgcggccg aggggcgcag
     9781 cccctggggg gatgggaggc ccgcgttagc gggccgggag ggttcgagaa gggggggcac
     9841 cccccttcgg cgtgcgcggt cacgcgcaca gggcgcagcc ctggttaaaa acaggtttat
     9901 aaatattggt ttaaaagcag gttaaaagac aggttagcgg tggccgaaaa acgggcggaa
     9961 acccttgcaa atgctggatt ttctgcctgt ggacagcccc tcaaatgtca ataggtgcgc
    10021 ccctcatctg tcagcactct gcccctcaag tgtcaaggat cgcgcccctc atctgtcagt
    10081 agtcgcgccc ctcaagtgtc aataccgcag ggcacttatc cccaggcttg tccacatcat
    10141 ctgtgggaaa ctcgcgtaaa atcaggcgtt ttcgccgatt tgcgaggctg gccagctcca
    10201 cgtcgccggc cgaaatcgag cctgcccctc atctgtcaac gccgcgccgg gtgagtcggc
    10261 ccctcaagtg tcaacgtccg cccctcatct gtcagtgagg gccaagtttt ccgcgaggta
    10321 tccacaacgc cggcggccgc ggtgtctcgc acacggcttc gacggcgttt ctggcgcgtt
    10381 tgcagggcca tagacggccg ccagcccagc ggcgagggca accagcccgg tgagcgtcgg
    10441 aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtacac tagtcgctgg ctgctgaacc
    10501 cccagccgga actgacccca caaggcccta gcgtttgcaa tgcaccaggt catcattgac
    10561 ccaggcgtgt tccaccaggc cgctgcctcg caactcttcg caggcttcgc cgacctgctc
    10621 gcgccacttc ttcacgcggg tggaatccga tccgcacatg aggcggaagg tttccagctt
    10681 gagcgggtac ggctcccggt gcgagctgaa atagtcgaac atccgtcggg ccgtcggcga
    10741 cagcttgcgg tacttctccc atatgaattt cgtgtagtgg tcgccagcaa acagcacgac
    10801 gatttcctcg tcgatcagga cctggcaacg ggacgttttc ttgccacggt ccaggacgcg
    10861 gaagcggtgc agcagcgaca ccgattccag gtgcccaacg cggtcggacg tgaagcccat
    10921 cgccgtcgcc tgtaggcgcg acaggcattc ctcggccttc gtgtaatacc ggccattgat
    10981 cgaccagccc aggtcctggc aaagctcgta gaacgtgaag gtgatcggct cgccgatagg
    11041 ggtgcgcttc gcgtactcca acacctgctg ccacaccagt tcgtcatcgt cggcccgcag
    11101 ctcgacgccg gtgtaggtga tcttcacgtc cttgttgacg tggaaaatga ccttgttttg
    11161 cagcgcctcg cgcgggattt tcttgttgcg cgtggtgaac agggcagagc gggccgtgtc
    11221 gtttggcatc gctcgcatcg tgtccggcca cggcgcaata tcgaacaagg aaagctgcat
    11281 ttccttgatc tgctgcttcg tgtgtttcag caacgcggcc tgcttggcct cgctgacctg
    11341 ttttgccagg tcctcgccgg cggtttttcg cttcttggtc gtcatagttc ctcgcgtgtc
    11401 gatggtcatc gacttcgcca aacctgccgc ctcctgttcg agacgacgcg aacgctccac
    11461 ggcggccgat ggcgcgggca gggcaggggg agccagttgc acgctgtcgc gctcgatctt
    11521 ggccgtagct tgctggacca tcgagccgac ggactggaag gtttcgcggg gcgcacgcat
    11581 gacggtgcgg cttgcgatgg tttcggcatc ctcggcggaa aaccccgcgt cgatcagttc
    11641 ttgcctgtat gccttccggt caaacgtccg attcattcac cctccttgcg ggattgcccc
    11701 gactcacgcc ggggcaatgt gcccttattc ctgatttgac ccgcctggtg ccttggtgtc
    11761 cagataatcc accttatcgg caatgaagtc ggtcccgtag accgtctggc cgtccttctc
    11821 gtacttggta ttccgaatct tgccctgcac gaataccagc gaccccttgc ccaaatactt
    11881 gccgtgggcc tcggcctgag agccaaaaca cttgatgcgg aagaagtcgg tgcgctcctg
    11941 cttgtcgccg gcatcgttgc gccacatcta ggtactaaaa caattcatcc agtaaaatat
    12001 aatattttat tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat
    12061 actgttcttc cccgatatcc tccctgatcg accggacgca gaaggcaatg tcataccact
    12121 tgtccgccct gccgcttctc ccaagatcaa taaagccact tactttgcca tctttcacaa
    12181 agatgttgct gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg
    12241 tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt
    12301 cgcaatccac atcggccaga tcgttattca gtaagtaatc caattcggct aagcggctgt
    12361 ctaagctatt cgtataggga caatccgata tgtcgatgga gtgaaagagc ctgatgcact
    12421 ccgcatacag ctcgataatc ttttcagggc tttgttcatc ttcatactct tccgagcaaa
    12481 ggacgccatc ggcctcactc atgagcagat tgctccagcc atcatgccgt tcaaagtgca
    12541 ggacctttgg aacaggcagc tttccttcca gccatagcat catgtccttt tcccgttcca
    12601 catcataggt ggtcccttta taccggctgt ccgtcatttt taaatatagg ttttcatttt
    12661 ctcccaccag cttatatacc ttagcaggag acattccttc cgtatctttt acgcagcggt
    12721 atttttcgat cagttttttc aattccggtg atattctcat tttagccatt tattatttcc
    12781 ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc
    12841 caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt
    12901 gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac cacaattatg
    12961 ggtgatgctg ccaacttact gatttagtgt atgatggtgt ttttgaggtg ctccagtggc
    13021 ttctgtttct atcagctgtc cctcctgttc agctactgac ggggtggtgc gtaacggcaa
    13081 aagcaccgcc ggacatcagc gctatctctg ctctcactgc cgtaaaacat ggcaactgca
    13141 gttcacttac accgcttctc aacccggtac gcaccagaaa atcattgata tggccatgaa
    13201 tggcgttgga tgccgggcaa cagcccgcat tatgggcgtt ggcctcaaca cgattttacg
    13261 tcacttaaaa aactcaggcc gcagtcggta actatgcggt gtgaaatacc gcacagatgc
    13321 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc
    13381 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
    13441 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
    13501 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
    13561 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
    13621 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
    13681 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
    13741 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
    13801 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
    13861 gacttatcgc cactggcagc aggtaacctc gcgcatacag ccgggcagtg acgtcatcgt
    13921 ctgcgcggaa atggacgggc ccccggcgcc agatctgggg aaccctgtgg ttggcatgca
    13981 catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg attattctaa
    14041 taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagtttg
    14101 tgaaccatca cccaaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
    14161 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
    14221 ggaagggaag aaagcgaaag gagcgggcgc cattcaggct gcgcaactgt tgggaagggc
    14281 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
    14341 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
    14401 aattgttaat taataggcct cgaggtcaac atggtggagc acgacacact tgtctactcc
    14461 aaaaatatca aagatacagt ctcagaagac caaagggcaa t
//
