LOCUS       sequence-479013-       11712 bp    DNA     circular SYN 18-JUL-2025
DEFINITION  3rd generation lentiviral transfer plasmid. Reporter for GLUT4 
            translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11712)
  AUTHORS   Lim CY, Bi X, Wu D, Kim JB, Gunning PW, Hong W, Han W
  TITLE     Tropomodulin3 is a novel Akt2 effector regulating insulin-stimulated
            GLUT4 exocytosis through cortical actin remodeling.
  JOURNAL   Nat Commun. 2015 Jan 9;6:5951. doi: 10.1038/ncomms6951.
  PUBMED    25575350
REFERENCE   2  (bases 1 to 11712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 18, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-01-9"; volume: "6:5951. doi"; pages: " 
            10.1038/ncomms6951"
COMMENT     Sequence Label: pLenti-myc-GLUT4-mCherry
FEATURES             Location/Qualifiers
     source          1..11712
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..20)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        192..571
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        573..771
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     726..746
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             789..969
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1016..1141
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1634..1867
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2052..2096
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2394..2511
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2576..3787
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3624..3643
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             4097..4126
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      5195..5204
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(5289..5300)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             5498..6199
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELY"
     primer_bind     complement(5640..5658)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5908..5927
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6102..6125
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    6358..6946
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6411..6431)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6829..6840)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6949..6965)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6950..6966)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7471..7651
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7677..7694)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7683..7907
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7953..8381
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8040..8059)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8250..8271
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8390..8410)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8395..8724
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8575..8710
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8637..8656
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8772..8819
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8838..9212
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9342..9463
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9379..9398)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9433..9452
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9512..9528)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9512..9528)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9525..9547)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9536..9552
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9560..9566,9567..9584,9585..9590))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9605..9626
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9743..9760)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9914..10502)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9994..10013)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10673..11464,11465..11533))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11296..11315
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11534..11638)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
       61 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
      121 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
      181 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      241 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      301 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      361 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      421 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      481 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      541 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      601 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      661 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      721 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg
      781 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
      841 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
      901 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
      961 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     1021 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     1081 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     1141 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     1201 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     1261 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     1321 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     1381 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     1441 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     1501 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     1561 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     1621 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1681 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1741 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1801 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1861 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1921 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1981 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     2041 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     2101 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     2161 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     2221 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     2281 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     2341 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
     2401 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2461 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     2521 tacagggaca gcagagatcc agtttggtta attaacccgt gtcggctcca gatctggcct
     2581 ccgcgccggg ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag cgctgccacg
     2641 tcagacgaag ggcgcagcga gcgtcctgat ccttccgccc ggacgctcag gacagcggcc
     2701 cgctgctcat aagactcggc cttagaaccc cagtatcagc agaaggacat tttaggacgg
     2761 gacttgggtg actctagggc actggttttc tttccagaga gcggaacagg cgaggaaaag
     2821 tagtcccttc tcggcgattc tgcggaggga tctccgtggg gcggtgaacg ccgatgatta
     2881 tataaggacg cgccgggtgt ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg
     2941 ttcttgtttg tggatcgctg tgatcgtcac ttggtgagta gcgggctgct gggctggccg
     3001 gggctttcgt ggccgccggg ccgctcggtg ggacggaagc gtgtggagag accgccaagg
     3061 gctgtagtct gggtccgcga gcaaggttgc cctgaactgg gggttggggg gagcgcagca
     3121 aaatggcggc tgttcccgag tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg
     3181 ttgaaacaag gtggggggca tggtgggcgg caagaaccca aggtcttgag gccttcgcta
     3241 atgcgggaaa gctcttattc gggtgagatg ggctggggca ccatctgggg accctgacgt
     3301 gaagtttgtc actgactgga gaactcggtt tgtcgtctgt tgcgggggcg gcagttatgg
     3361 cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg cgccctcgtc gtgtcgtgac
     3421 gtcacccgtt ctgttggctt ataatgcagg gtggggccac ctgccggtag gtgtgcggta
     3481 ggcttttctc cgtcgcagga cgcagggttc gggcctaggg taggctctcc tgaatcgaca
     3541 ggcgccggac ctctggtgag gggagggata agtgaggcgt cagtttcttt ggtcggtttt
     3601 atgtacctat cttcttaagt agctgaagct ccggttttga actatgcgct cggggttggc
     3661 gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg taatcatttg ggtcaatatg
     3721 taattttcag tgttagacta gtaaattgtc cgctaaattc tggccgtttt tggctttttt
     3781 gttagacgaa gcttgggctg caggtcgact ctagaggatc gaattcttcg aagctagcgc
     3841 taccggactc agatctcgag acaagatgcc gtcgggtttc cagcagatcg gctctgacga
     3901 tggggaaccc cctcggcagc gagtgactgg aacactggtc ctagctgtat tctcagctgt
     3961 gcttggctcc cttcagtttg gctataacat tggggttatc aatgccccac agaaggtgat
     4021 tgaacagagc tacaatgcaa cgtggctggg taggcaaggt cccggacgcg cgtcgacacc
     4081 ggtacgcgaa gccgaagaac agaaactgat ctctgaagaa gacctgctga agcgcgtagc
     4141 cgggggaccg gattccatcc cacaaggcac cctcactacg ctctgggctc tctccgtggc
     4201 catcttctct gtgggtggca tgatctcttc ctttctcatt ggcatcattt ctcaatggtt
     4261 gggaaggaaa agggctatgc tggccaacaa tgtcttggcc gtgttggggg gcgccctcat
     4321 gggcctagcc aatgccgcgg cctcctatga gatactcatt cttggacggt tcctcattgg
     4381 cgcctactca gggctaacat cagggctggt gcccatgtat gtgggagaaa tcgcccccac
     4441 tcatcttcgg ggtgccttgg gaacactcaa ccaactggcc atcgtcattg gcattctggt
     4501 tgcccaggtg ctgggcttgg agtctatgct gggcacagct accctgtggc cactgcttct
     4561 ggctctcaca gtactccctg ctctcctgca gctgattctg ctgcccttct gtcctgagag
     4621 ccccagatac ctctacatca tccggaacct ggaggggcct gcccgaaaga gtctaaagcg
     4681 cctgaccggc tgggctgatg tgtctgacgc actagctgag ctgaaggatg agaaacggaa
     4741 gttggagaga gagcgtccaa tgtccttgct ccagctcctg ggcagccgca cccaccggca
     4801 gcctctgatc atcgcagtgg tgctgcagct gagccaacag ctctcaggca tcaatgctgt
     4861 tttctactat tcaaccagca tcttcgagtc ggctggggtg ggacagccag cctacgccac
     4921 cataggagct ggtgtggtca atacggtctt cacgttggtc tcggtgctct tagtagaacg
     4981 agctggacga cggacactcc atctgttggg cctggccggc atgtgtggct gtgccatctt
     5041 gatgaccgtg gctctgctgc tgctggaacg ggttccagcc atgagctatg tctccatcgt
     5101 ggccatattt ggctttgtgg ccttctttga gattggccct ggccccattc cctggttcat
     5161 tgtggcagag ctcttcagcc agggcccccg cccagccgcc atggctgtcg ctggtttctc
     5221 caactggacc tgtaacttca ttgtcggcat gggtttccag tatgttgcgg atgctatggg
     5281 tccttacgtc ttccttctat ttgccgtcct cctgcttggc ttcttcatct tcaccttcct
     5341 aaaagtgcct gaaaccagag gccggacgtt tgaccagatc tcagctgcct tccgacggac
     5401 accttccctt ttagagcagg aggtgaaacc cagtacagaa cttgaatact tagggccaga
     5461 tgagaatgac ggggatccac cggaattcac aaccctggtg agcaagggcg aggaggacaa
     5521 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
     5581 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     5641 caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccctca
     5701 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
     5761 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
     5821 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
     5881 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
     5941 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
     6001 gctgaagctg aaggacggcg gccactacga cgccgaggtc aagaccacct acaaggccaa
     6061 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagctggaca tcacctccca
     6121 caacgaggac tacaccatcg tggaacagta cgagcgcgcc gagggccgcc actccaccgg
     6181 cggcatggat gagctgtacg tacaagtaaa gcggccgcga ctctagatca taatcagcca
     6241 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     6301 gaaacataaa atgaatgcaa ttgttgttgt taacaattcg atatcaagct tatcgataat
     6361 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     6421 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     6481 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     6541 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     6601 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     6661 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     6721 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     6781 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     6841 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     6901 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga
     6961 cctcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
     7021 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag
     7081 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     7141 aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg
     7201 atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc
     7261 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta
     7321 gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg
     7381 gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac
     7441 atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc
     7501 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     7561 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     7621 cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc
     7681 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     7741 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     7801 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     7861 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     7921 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     7981 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     8041 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     8101 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     8161 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     8221 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     8281 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     8341 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     8401 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     8461 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     8521 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     8581 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     8641 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     8701 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     8761 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
     8821 aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg
     8881 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
     8941 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
     9001 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
     9061 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
     9121 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
     9181 gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg
     9241 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     9301 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     9361 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     9421 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     9481 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     9541 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     9601 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     9661 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     9721 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     9781 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     9841 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     9901 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     9961 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    10021 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    10081 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
    10141 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    10201 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    10261 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    10321 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
    10381 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    10441 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    10501 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    10561 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    10621 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    10681 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    10741 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    10801 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    10861 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    10921 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    10981 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    11041 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    11101 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    11161 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    11221 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    11281 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    11341 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    11401 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    11461 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    11521 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    11581 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    11641 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
    11701 gtgcactctc ag
//
