LOCUS       sequence-479016-        4983 bp    DNA     circular SYN 18-JUL-2025
DEFINITION  Gateway entry vector with integrin beta1 wild-type (WT) without a 
            stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Conway JRW, Joshi O, Kaivola J, Follain G, Gounis M, Kuhl D, Ivaska 
            J
  TITLE     Dynamic regulation of integrin beta1 phosphorylation supports 
            invasion of breast cancer cells.
  JOURNAL   Nat Cell Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4.
  PUBMED    40419795
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 18, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4."
COMMENT     Sequence Label: pENTR221-ITGB1(no stop)
FEATURES             Location/Qualifiers
     source          1..4983
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3108..3207)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3224..3243)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3225..3243)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3248..3264)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3248..3264)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3366..3385)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3377..4186
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3444..3463)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4333..4921
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4822..4841
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctgg cgccgaccaa ttcagtcgac tggatccggt accaccgcca tgaatttaca
      721 accaattttc tggattggac tgatcagttc agtttgctgt gtgtttgctc aaacagatga
      781 aaatagatgt ttaaaagcaa atgccaaatc atgtggagaa tgtatacaag cagggccaaa
      841 ttgtgggtgg tgcacaaatt caacattttt acaggaagga atgcctactt ctgcacgatg
      901 tgatgattta gaagccttaa aaaagaaggg ttgccctcca gatgacatag aaaatcccag
      961 aggctccaaa gatataaaga aaaataaaaa tgtaaccaac cgtagcaaag gaacagcaga
     1021 gaagctcaag ccagaggata ttactcagat ccaaccacag cagttggttt tgcgattaag
     1081 atcaggggag ccacagacat ttacattaaa attcaagaga gctgaagact atcccattga
     1141 cctctactac cttatggacc tgtcttactc aatgaaagac gatttggaga atgtaaaaag
     1201 tcttggaaca gatctgatga atgaaatgag gaggattact tcggacttca gaattggatt
     1261 tggctcattt gtggaaaaga ctgtgatgcc ttacattagc acaacaccag ctaagctcag
     1321 gaacccttgc acaagtgaac agaactgcac cagcccattt agctacaaaa atgtgctcag
     1381 tcttactaat aaaggagaag tatttaatga acttgttgga aaacagcgca tatctggaaa
     1441 tttggattct ccagaaggtg gtttcgatgc catcatgcaa gttgcagttt gtggatcact
     1501 gattggctgg aggaatgtta cacggctgct ggtgttttcc acagatgccg ggtttcactt
     1561 tgctggagat gggaaacttg gtggcattgt tttaccaaat gatggacaat gtcacctgga
     1621 aaataatatg tacacaatga gccattatta tgattatcct tctattgctc accttgtcca
     1681 gaaactgagt gaaaataata ttcagacaat ttttgcagtt actgaagaat ttcagcctgt
     1741 ttacaaggag ctgaaaaact tgatccctaa gtcagcagta ggaacattat ctgcaaattc
     1801 tagcaatgta attcagttga tcattgatgc atacaattcc ctttcctcag aagtcatttt
     1861 ggaaaacggc aaattgtcag aaggagtaac aataagttac aaatcttact gcaagaacgg
     1921 ggtgaatgga acaggggaaa atggaagaaa atgttccaat atttccattg gagatgaggt
     1981 tcaatttgaa attagcataa cttcaaataa gtgtccaaaa aaggattctg acagctttaa
     2041 aattaggcct ctgggcttta cggaggaagt agaggttatt cttcagtaca tctgtgaatg
     2101 tgaatgccaa agcgaaggca tccctgaaag tcccaagtgt catgaaggaa atgggacatt
     2161 tgagtgtggc gcgtgcaggt gcaatgaagg gcgtgttggt agacattgtg aatgcagcac
     2221 agatgaagtt aacagtgaag acatggatgc ttactgcagg aaagaaaaca gttcagaaat
     2281 ctgcagtaac aatggagagt gcgtctgcgg acagtgtgtt tgtaggaaga gggataatac
     2341 aaatgaaatt tattctggca aattctgcga gtgtgataat ttcaactgtg atagatccaa
     2401 tggcttaatt tgtggaggaa atggtgtttg caagtgtcgt gtgtgtgagt gcaaccccaa
     2461 ctacactggc agtgcatgtg actgttcttt ggatactagt acttgtgaag ccagcaacgg
     2521 acagatctgc aatggccggg gcatctgcga gtgtggtgtc tgtaagtgta cagatccgaa
     2581 gtttcaaggg caaacgtgtg agatgtgtca gacctgcctt ggtgtctgtg ctgagcataa
     2641 agaatgtgtt cagtgcagag ccttcaataa aggagaaaag aaagacacat gcacacagga
     2701 atgttcctat tttaacatta ccaaggtaga aagtcgggac aaattacccc agccggtcca
     2761 acctgatcct gtgtcccatt gtaaggagaa ggatgttgac gactgttggt tctattttac
     2821 gtattcagtg aatgggaaca acgaggtcat ggttcatgtt gtggagaatc cagagtgtcc
     2881 cactggtcca gacatcattc caattgtagc tggtgtggtt gctggaattg ttcttattgg
     2941 ccttgcgtta ctcctcattt ggaagctttt aatgataatt catgacagaa gggagtttgc
     3001 taaatttgaa aaggagaaaa tgaatgccaa atgggacacg ggtgaaaatc ctatttataa
     3061 gagtgccgta acaactgtgg tcaatccgaa gtatgaggga aaacgctacc cagctttctt
     3121 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
     3181 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
     3241 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     3301 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     3361 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
     3421 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     3481 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     3541 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     3601 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     3661 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     3721 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     3781 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     3841 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     3901 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     3961 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     4021 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     4081 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     4141 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
     4201 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc
     4261 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
     4321 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     4381 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     4441 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     4501 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4561 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4621 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4681 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4741 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4801 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4861 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4921 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4981 gtt
//
