LOCUS       sequence-479099-        4740 bp    DNA     circular SYN 21-AUG-2025
DEFINITION  Retroviral vector containing human BAP1 and Thy1.1 selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Balasubramani A, Larjo A, Bassein JA, Chang X, Hastie RB, Togher SM,
            Lahdesmaki H, Rao A
  TITLE     Cancer-associated ASXL1 mutations may act as gain-of-function 
            mutations of the ASXL1-BAP1 complex.
  JOURNAL   Nat Commun. 2015 Jun 22;6:7307. doi: 10.1038/ncomms8307.
  PUBMED    26095772
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 21, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-06-22"; volume: "6:7307. doi"; pages: " 
            10.1038/ncomms8307"
COMMENT     Sequence Label: BAP1
FEATURES             Location/Qualifiers
     source          1..4740
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(919..946)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1038..1124)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1173..1195
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1187..1204
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1188..1204
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1220..1319
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    2513..2524
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     protein_bind    complement(3516..3615)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3632..3651)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3633..3651)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3656..3672)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3656..3672)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3774..3793)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3785..4594
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3852..3871)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga
      901 agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc
      961 ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc
     1021 aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
     1081 aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc
     1141 tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc
     1201 cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga cctgttcgtt
     1261 gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa aagcaggctc
     1321 caccatgaat aagggctggc tggagctgga gagcgaccca ggcctcttca ccctgctcgt
     1381 ggaagatttc ggtgtcaagg gggtgcaagt ggaggagatc tacgaccttc agagcaaatg
     1441 tcagggccct gtatatggat ttatcttcct gttcaaatgg atcgaagagc gccggtcccg
     1501 gcgaaaggtc tctaccttgg tggatgatac gtccgtgatt gatgatgata ttgtgaataa
     1561 catgttcttt gcccaccagc tgatacccaa ctcttgtgca actcatgcct tgctgagcgt
     1621 gctcctgaac tgcagcagcg tggacctggg acccaccctg agtcgcatga aggacttcac
     1681 caagggtttt agccctgaga gcaaaggata tgcgattggc aatgccccgg agttggccaa
     1741 ggcccataat agccatgcca ggcccgagcc acgccacctc cctgagaagc agaatggcct
     1801 tagtgcagtg cggaccatgg aggcgttcca ctttgtcagc tatgtgccta tcacaggccg
     1861 gctctttgag ctggatgggc tgaaggtcta ccccattgac catgggccct ggggggagga
     1921 cgaggagtgg acagacaagg cccggcgggt catcatggag cgtatcggcc tcgccactgc
     1981 aggggagccc taccacgaca tccgcttcaa cctgatggca gtggtgcccg accgcaggat
     2041 caagtatgag gccaggctgc atgtgctgaa ggtgaaccgt cagacagtac tagaggctct
     2101 gcagcagctg ataagagtaa cacagccaga gctgattcag acccacaagt ctcaagagtc
     2161 acagctgcct gaggagtcca agtcagccag caacaagtcc ccgctggtgc tggaagcaaa
     2221 cagggcccct gcagcctctg agggcaacca cacagatggt gcagaggagg cggctggttc
     2281 atgcgcacaa gccccatccc acagccctcc caacaaaccc aagctagtgg tgaagcctcc
     2341 aggcagcagc ctcaatgggg ttcaccccaa ccccactccc attgtccagc ggctgccggc
     2401 ctttctagac aatcacaatt atgccaagtc ccccatgcag gaggaagaag acctggcggc
     2461 aggtgtgggc cgcagccgag ttccagtccg cccaccccag cagtactcag atgatgagga
     2521 tgactatgag gatgacgagg aggatgacgt gcagaacacc aactctgccc ttaggtataa
     2581 ggggaaggga acagggaagc caggggcatt gagcggttct gctgatgggc aactgtcagt
     2641 gctgcagccc aacaccatca acgtcttggc tgagaagctc aaagagtccc agaaggacct
     2701 ctcaattcct ctgtccatca agactagcag cggggctggg agtccggctg tggcagtgcc
     2761 cacacactcg cagccctcac ccacccccag caatgagagt acagacacgg cctctgagat
     2821 cggcagtgct ttcaactcgc cactgcgctc gcctatccgc tcagccaacc cgacgcggcc
     2881 ctccagccct gtcacctccc acatctccaa ggtgcttttt ggagaggatg acagcctgct
     2941 gcgtgttgac tgcatacgct acaaccgtgc tgtccgtgat ctgggtcctg tcatcagcac
     3001 aggcctgctg cacctggctg aggatggggt gctgagtccc ctggcgctga cagagggtgg
     3061 gaagggttcc tcgccctcca tcagaccaat ccaaggcagc caggggtcca gcagcccagt
     3121 ggagaaggag gtcgtggaag ccacggacag cagagagaag acggggatgg tgaggcctgg
     3181 cgagcccttg agtggggaga aatactcacc caaggagctg ctggcactgc tgaagtgtgt
     3241 ggaggctgag attgcaaact atgaggcgtg cctcaaggag gaggtagaga agaggaagaa
     3301 gttcaagatt gatgaccaga gaaggaccca caactacgat gagttcatct gcacctttat
     3361 ctccatgctg gctcaggaag gcatgctggc caacctagtg gagcagaaca tctccgtgcg
     3421 gcggcgccaa ggggtcagca tcggccggct ccacaagcag cggaagcctg accggcggaa
     3481 acgctctcgc ccctacaagg ccaagcgcca gtaggaccca gctttcttgt acaaagttgg
     3541 cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa
     3601 taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat
     3661 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga
     3721 taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg
     3781 tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc
     3841 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
     3901 tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
     3961 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct
     4021 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
     4081 ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
     4141 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
     4201 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt
     4261 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga
     4321 aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact
     4381 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg
     4441 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc
     4501 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt
     4561 gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca
     4621 ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa
     4681 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
