LOCUS       sequence-479101-        3278 bp    DNA     circular SYN 21-JUL-2025
DEFINITION  Entry vector with cGAL[cGAL(DBD)-linker-QFAD]-tbb-3'UTR in slot 5 
            (for SapTrap assembly).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Khan H, Huang X, Raj V, Wang H
  TITLE     A versatile site-directed gene trap strategy to manipulate gene 
            activity and control gene expression in Caenorhabditis elegans.
  JOURNAL   PLoS Genet. 2025 Jan 22;21(1):e1011541. doi: 
            10.1371/journal.pgen.1011541. eCollection 2025 Jan.
  PUBMED    39841730
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 21, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2025-01-22"; volume: "21(1):e1011541. doi"; pages: "
            10.1371/journal.pgen.1011541. eCollection 2025 Jan"
COMMENT     Sequence Label: pHW621
FEATURES             Location/Qualifiers
     source          1..3278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1..816)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     724..743
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(817..919)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(920..951)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     terminator      2060..2383
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(2572..2589)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2572..2588)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2629..3216
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3117..3136
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3228..3255)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ttagaaaaac tcatcgagca tcaagtgaaa ctgcaattta ttcatatcag gattatcaat
       61 accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca
      121 taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc
      181 tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac
      241 tgaatccggt gagaatggca agagtttatg catttctttc cagacttgtt caacaggcca
      301 gccattacgc tcgtcatcaa aatcactcgc accaaccaaa ccgttattca ttcgtgattg
      361 cgcctgagcg agacgaaata cgcgatcgcc gttaaaagga caattacaaa caggaatcga
      421 atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata
      481 ttcttctaat acctggaatg ctgttttccc tgggatcgca gtggtgagta accatgcatc
      541 atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt
      601 tagcctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa
      661 caactctggc gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac
      721 attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg
      781 cttcaagcaa gacgtttccc gttgaatatg gctcatttta gcttccttag ctcctgaaaa
      841 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
      901 acctcttacg tgccgatcaa gtcaaaagcc tccggtcgga ggcttttgac tttctgctat
      961 ggaggtcagg tatgattttt gaattcgctc ttcgatggga ccaaagaaga agcgtaaggc
     1021 ctccatgaag ctcttgtcct ccatggagca agcctgcgac atctgccgtc tcaagaaact
     1081 ccgttgctcc aaggagaagc caaagtgcgc caagtgcctc aagaacaact ccgagtgccg
     1141 ttactcccca aaggccatgc gttccccact gactcgtgcc cacctcaccg aggtcgagtc
     1201 ccgtctcgag aagctcgaag acctcttcct cctcatgttc ccacgtgaga acctggactc
     1261 catcctcaac atggactccc tcgacgaggt caaggtaagt ttaaacatat atatactaac
     1321 taaccctgat tatttaaatt ttcaggtcat gctcaagcaa ctctacatgc aagactacgg
     1381 agacaccgac gctgccatgg actccctcac cccaatggag aacggaatgt ctgagggacc
     1441 acgtaagcac atgaagaccg ccacctcatc cccaaaagtc gacgaggacg gatcccaatc
     1501 ccagctctcc gtctccaagg gttctggatc tggatctgga ggttccggtc gtcaacttga
     1561 gctcccacca accgccaccg ccaccgccag tatcatgcca cacgtcatgg agcaaccact
     1621 ctccacctcc atcaacccag tcaacgaccg tttcaacgga ataccaaacc caaccccata
     1681 caactccgac gccgccctcg acgccatcac ccaaaccaac gactacggat ccgtcaacac
     1741 ccacggaatc ctctccacct acccaccacc agccacccac ctcaacgagg cctccgtcgc
     1801 cctcgcacca ggaggagcac caccacgtcc accaccacca tacgtcgact ccaccaccaa
     1861 ccacccacca taccattcca acctcgtccc aatggccaac ttcggatact ccaccgtcga
     1921 ctacgacgcc atggtcgacg acctcgcctc catcgagtac accgacgccg tcgacgtcga
     1981 cccacaattc atgaccaacc tcgggttcgt cccaggatgc aacttctccg acatcaacac
     2041 ctatgagcaa taaacgtaaa tgcaaaatcc tttcaagcat tcccttcttc tctatcactc
     2101 ttctttcttt ttgtcaaaaa attctctcgc taatttattt gcttttttaa tgttattatt
     2161 ttatgacttt ttatagtcac tgaaaagttt gcatctgagt gaagtgaatg ctatcaaaat
     2221 gtgattctgt ctgatgtact ttcacaatct ctcttcaatt ccattttgaa gtgctttaaa
     2281 cccgaaaggt tgagaaaaat gcgagcgctc aaatatttgt attgtgttcg ttgagtgacc
     2341 caacaaaaag aggaaacttt attgtgccgc caagaaaaaa gtctcaaagg gaagagcaag
     2401 cttggatcga cgagagcagc gcgactggat ctgtcgcccg tctcaaacgc aaccctccgg
     2461 cggtcgcata tcattcagga cgagcctcag actccagcgt aactggactg caatcaactc
     2521 actggctcac cttccggtcc acgatcagct agaatcaagc tgactagata aactggccgt
     2581 cgttttacac gggtgggcct ttcttcggta gaaaatcaaa ggatcttctt gagatccttt
     2641 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     2701 tttgccggat caagagctac caactctttt tccgaggtaa ctggcttcag cagagcgcag
     2761 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     2821 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     2881 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     2941 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     3001 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     3061 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     3121 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcatcgattt
     3181 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc agaaaggccc
     3241 acccgaaggt gagccaggtg attacattta ggtcctca
//
