LOCUS       sequence-479108-        9403 bp    DNA     circular SYN 21-JUL-2025
DEFINITION  OMEGAOff-KRK mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9403)
  AUTHORS   Kannan S, Altae-Tran H, Zhu S, Xu P, Strebinger D, Oshiro R, Faure 
            G, Moeller L, Pham J, Mears KS, Ni HM, Macrae RK, Zhang F
  TITLE     Evolution-guided protein design of IscB for persistent epigenome 
            editing in vivo.
  JOURNAL   Nat Biotechnol. 2025 May 7. doi: 10.1038/s41587-025-02655-3.
  PUBMED    40335752
REFERENCE   2  (bases 1 to 9403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 21, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2025 May 7. doi: 10.1038/s41587-025-02655-3."
COMMENT     Sequence Label: pHS1993
FEATURES             Location/Qualifiers
     source          1..9403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     612..631
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(850..954)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1029..1048)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1220..1599
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1600..1803
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1754..1774
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1848..1867
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1848..1866
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1902..1911
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1917..1937
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5649..5669
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5721..5906
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     CDS             5907..5933
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(5979..5996)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5985..6209
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6255..6683
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6342..6361)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6552..6573
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6692..6712)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6697..7026
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6877..7012
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6939..6958
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7093..7887
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7147..7166)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7757..7776
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8061..8182
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8098..8117)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8152..8171
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8231..8247)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8231..8247)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8244..8266)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8255..8271
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8279..8285,8286..8303,8304..8309))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8324..8345
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8462..8479)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8633..9221)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8713..8732)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9392..9403,1..780,781..849))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
       61 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
      121 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
      181 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
      241 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
      301 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
      361 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
      421 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
      481 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
      541 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
      601 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
      661 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
      721 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
      781 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
      841 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
      901 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
      961 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
     1021 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
     1081 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
     1141 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
     1201 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
     1261 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     1321 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     1381 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     1441 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     1501 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     1561 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     1621 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     1681 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     1741 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     1801 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
     1861 tatagggaga cccaagctgg ctagcgttta aacttaagct tgccaccatg ggatctccaa
     1921 aaaagaaacg gaaggtggga tccaaccatg accaggaatt tgacccccca aaggtttacc
     1981 cacctgtgcc agctgagaag aggaagccca tccgcgtgct gtctctcttt gatgggattg
     2041 ctacagggct cctggtgctg aaggacctgg gcatccaagt ggaccgctac attgcctccg
     2101 aggtgtgtga ggactccatc acggtgggca tggtgcggca ccagggaaag atcatgtacg
     2161 tcggggacgt ccgcagcgtc acacagaagc atatccagga gtggggccca ttcgacctgg
     2221 tgattggagg cagtccctgc aatgacctct ccattgtcaa ccctgcccgc aagggacttt
     2281 atgagggtac tggccgcctc ttctttgagt tctaccgcct cctgcatgat gcgcggccca
     2341 aggagggaga tgatcgcccc ttcttctggc tctttgagaa tgtggtggcc atgggcgtta
     2401 gtgacaagag ggacatctcg cgatttcttg agtctaaccc cgtgatgatt gacgccaaag
     2461 aagtgtctgc tgcacacagg gcccgttact tctggggtaa ccttcctggc atgaacaggc
     2521 ctttggcatc cactgtgaat gataagctgg agctgcaaga gtgtctggag cacggcagaa
     2581 tagccaagtt cagcaaagtg aggaccatta ccaccaggtc aaactctata aagcagggca
     2641 aagaccagca tttccccgtc ttcatgaacg agaaggagga catcctgtgg tgcactgaaa
     2701 tggaaagggt gtttggcttc cccgtccact acacagacgt ctccaacatg agccgcttgg
     2761 cgaggcagag actgctgggc cgatcgtgga gcgtgccggt catccgccac ctcttcgctc
     2821 cgctgaagga atattttgct tgtgtgtcta gcggcaatag taacgctaac agccgcgggc
     2881 cgagcttcag cagcggcctg gtgccgttaa gcttgcgcgg cagccatatg ggccctatgg
     2941 agatatacaa gacagtgtct gcatggaaga gacagccagt gcgggtactg agcctcttca
     3001 gaaacatcga caaggtacta aagagtttgg gcttcttgga aagcggttct ggttctgggg
     3061 gaggaacgct gaagtacgtg gaagatgtca caaatgtcgt gaggagagac gtggagaaat
     3121 ggggcccctt tgacctggtg tacggctcga cgcagcccct aggcagctct tgtgatcgct
     3181 gtcccggctg gtacatgttc cagttccacc ggatcctgca gtatgcgctg cctcgccagg
     3241 agagtcagcg gcccttcttc tggatattca tggacaatct gctgctgact gaggatgacc
     3301 aagagacaac tacccgcttc cttcagacag aggctgtgac cctccaggat gtccgtggca
     3361 gagactacca gaatgctatg cgggtgtgga gcaacattcc agggctgaag agcaagcatg
     3421 cgcccctgac cccaaaggaa gaagagtatc tgcaagccca agtcagaagc aggagcaagc
     3481 tggacgcccc gaaagttgac ctcctggtga agaactgcct tctcccgctg agagagtact
     3541 tcaagtattt ttctcaaaac tcacttcctc ttggagggcc gagctctggc gcacccccac
     3601 caagtggagg gtctcctgcc gggtccccaa catctactga agaaggcacc agcgaatccg
     3661 caacgcccga gtcaggccct ggtacctcca cagaaccatc tgaaggtagt gcgcctggtt
     3721 ccccagctgg aagccctact tccaccgaag aaggcacgtc aaccgaacca agtgaaggat
     3781 ctgcccctgg gaccagcact gaaccatctg agatgagata cgtgtacgtg ctggatgtgg
     3841 acggcaagcc tctgatgcct acatgcaggt tcggcaaggt gcggagaatg ctgaaatctg
     3901 gccaagctaa ggccgtggac accctgcctt tcaccatcca gctgacctac aagcccagaa
     3961 ccagaatcct gcaacctgtg accctgggcc aggcccccgg cagaaccaat atcggaatgg
     4021 ccgccgtgcg gttcgacggc aaggaactgg gaagattcca ctgcatcaca agaaacaagg
     4081 aaatccctaa gctgatggcc gacagaatgg cagccagaaa ggcctcccgg agaggcgaga
     4141 gactggccag aaagcgcctg gcccggaaac tgcacaccac cgccaagcac ctgaatggca
     4201 gaatcctgcc aggatgtagc aaacccatcg ccgtgaagga catcatcaac acagagagca
     4261 gattcaacaa cagaatcctg accaaatgca aagtgtgcgg caagaacacc cctctgagaa
     4321 gaaacgtgcg ggaactcctg ctggaaaaca tcgtgcggtt tctgcctctg gaaagcgagc
     4381 tgaaggaaac actcaagaga accatcctgg aaggccagca gggcaatatc aacaagctgt
     4441 tcaggaaact taaattcaat caaaaggatt ggcctggcaa aaacctgacc gatatcgcta
     4501 agaataagct gccaggccgg ctgcccttct gcaaggagca cttcgccgag aacgagaagt
     4561 tcaccaccat cgagaagagc accttccggc tgacccctac cgccacccag ctgctgcgga
     4621 cccacatcaa cctgtttaga aagctgagcg gcatcctgcc cgtgaccgac gtggccgtcg
     4681 agctgaacaa gttcgccttt atgcagctgg acaaccctga gatgaagaag cgggaaatcg
     4741 acttctgcca cggcccactg tgcggcaccg gcggcctgga agccgctgtt aaggagcagc
     4801 aggatggaaa atgcctgctg tgtggaaaag aatctatcgg ccactaccac gccatcgtgc
     4861 ctaggtcccg tagaggcagc aacaccatcg ctaatatcgc cggcctttgt cctaagtgcc
     4921 acgagctcgt gcacaaggac gccgatacag ccgagtctct gacagagatg aaaaccggcc
     4981 tgatgaagaa gtatggaggc accagcgtgc tgaaccagat catccccaag ctggtgcgca
     5041 ccctggccga cctgtttcct ggccacttcc acgtgacaaa cggctggaac accaaggagt
     5101 tcagagagaa acatcatctg gaaaaggatc acgacgtgga tgcttactgc atcgcctgca
     5161 gccacctgaa gcctgaggaa accctggtgg aaactgagcc tttcgagatc ctgcagttca
     5221 gaaagcacaa cagagccatc attcaccacc agaccgagcg gacgtacaag ctggacggcg
     5281 tgacagtggc taaaaataga aagaaacgga tggaacagaa aaccgacagc ctggaagatt
     5341 ggtacgtgga catggccaag gaacacggca aaacacaggc cgatgccatg cggagcagac
     5401 tgaccgtgaa aaagagcaca cggtattaca acacccctgg cagaatgatg ccaggcacag
     5461 ttttcctgta cgagggcaag agatacgtga tgaccggcca gattacaaac ggcaagtact
     5521 acagagccta cggccaggag aagcggaact tccccgctgt caaggtgagg attctgacaa
     5581 agaacaccgg actagtcttt gtggcctctg gcggctcaaa aagaaccgcc gacggcagcg
     5641 aattcgagcc caagaagaag aggaaagtcg gatccggcgg tggcggaggg atggatgcta
     5701 agtcactaac tgcctggtcc cggacactgg tgaccttcaa ggatgtattt gtggacttca
     5761 ccagggagga gtggaagctg ctggacactg ctcagcagat cgtgtacaga aatgtgatgc
     5821 tggagaacta taagaacctg gtttccttgg gttatcagct tactaagcca gatgtgatcc
     5881 tccggttgga gaagggagaa gagccctacc catacgatgt tccagattac gcttgaggcc
     5941 gctcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     6001 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     6061 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     6121 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     6181 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     6241 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     6301 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     6361 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     6421 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     6481 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     6541 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     6601 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     6661 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     6721 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     6781 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     6841 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     6901 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     6961 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     7021 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga
     7081 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     7141 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     7201 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     7261 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     7321 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     7381 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     7441 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     7501 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     7561 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     7621 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     7681 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     7741 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     7801 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     7861 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     7921 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     7981 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     8041 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     8101 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     8161 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     8221 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     8281 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     8341 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     8401 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     8461 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     8521 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     8581 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     8641 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     8701 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     8761 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     8821 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     8881 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     8941 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     9001 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     9061 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     9121 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     9181 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     9241 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     9301 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     9361 aaagtatata tgagtaaact tggtctgaca gttaccaatg ctt
//
