LOCUS       sequence-479130-       14443 bp    DNA     circular SYN 04-AUG-2025
DEFINITION  Expresses full-length, driven by its own promoter MALAT1 with 
            stability-compromised ENE(C8351G) and an 11-nt insertion 
            (cgctcgacgta)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14443)
  AUTHORS   Che R, Panah M, Mirani B, Knowles K, Ostapovich A, Majumdar D, Chen 
            X, DeSimone J, White W, Noonan M, Luo H, Alexandrov A
  TITLE     Identification of human pathways acting on nuclear non-coding RNAs 
            using the Mirror forward genetic approach.
  JOURNAL   Nat Commun. 2025 May 22;16(1):4741. doi: 10.1038/s41467-025-59998-3.
  PUBMED    40399278
REFERENCE   2  (bases 1 to 14443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-05-22"; volume: "16(1):4741. doi"; pages: " 
            10.1038/s41467-025-59998-3"
COMMENT     Sequence Label: pcDNA5/FRT/MALAT1[C8351G] (pAVA3169)
FEATURES             Location/Qualifiers
     source          1..14443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(72..140,141..932)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(290..309)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1103..1691
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1592..1611
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1845..1862
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1979..2000
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2015..2020,2021..2038,2039..2045)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2053..2069
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2058..2080
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2077..2093
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2077..2093
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    2142..2263
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2153..2172)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2207..2226
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2431..3453)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             complement(2431..3453)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     protein_bind    complement(3461..3508)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(14410..14443,1..71)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
       61 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      121 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      181 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      241 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      301 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      361 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      421 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      481 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      541 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      601 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      661 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      721 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      781 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      841 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      901 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
      961 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1021 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1081 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1141 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1201 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     1261 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1321 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1381 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1441 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1501 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1561 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1621 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1681 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1741 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1801 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1861 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1921 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     1981 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2041 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2101 acgccaagct ctagctagag gtcgacggta tacagacatg ataagataca ttgatgagtt
     2161 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     2221 tattgcttta tttgtaacca ttataagctg caataaacaa gttggggtgg gcgaagaact
     2281 ccagcatgag atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga
     2341 agcccaacct ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg
     2401 tcgcttggtc ggtcatttcg aaagtacgtg ctattccttt gccctcggac gagtgctggg
     2461 gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct
     2521 tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca
     2581 tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg
     2641 gtcaagacca atgcggagca tatacgcccg gagccgcggc gatcctgcaa gctccggatg
     2701 cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa
     2761 gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc
     2821 tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg
     2881 ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga
     2941 cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc
     3001 gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa
     3061 cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atggcctccg cgaccggctg
     3121 cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg
     3181 ggagatgcaa taggtcaggc tctcgctgaa ttccccaatg tcaagcactt ccggaatcgg
     3241 gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca
     3301 gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc
     3361 ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt
     3421 ctcgacagac gtcgcggtga gttcaggctt tttggccaag gaagttccta tactttctag
     3481 agaataggaa cttcggaata ggaacttcta ggctcgagga ttcaagaaca gcttctgcta
     3541 ttaattagct gtgtgtcatt tcaggcaaat cacaaaatct ccaggccccc aaatccccat
     3601 ttgcgaaacg aggtgacctg cctcagtttt ccttcatcca gcgataaaat tctactaaaa
     3661 aatttccaac cccagagcaa gcatcaggac tgtgcctgaa gggatccggc tgcatctcag
     3721 taatattcca ctcttacagg cttaaaaata aaaaagaaaa gaaaagaaaa caagaacagt
     3781 ttcaccagcg tcaattgaga acagctgctt caacaggccc tgctttatgt gggcaggtgg
     3841 ggacagggag ttggcccaga aaacaggacc ctcatttcct ggagccccca gggcagctcc
     3901 ccaacaccgt acacaacctg catcatttac aggagccaaa ggagttttag aaatcaactt
     3961 catagagttg ctgtatcatt gtgttagttt tgccatccta acctatacag cgtcactaat
     4021 ctctcccctc ggagttgact gcctaaaaac aagccatgga tacaggttct aaagacccgg
     4081 gggtgaggga gcggagaggg tgggtgtcac caccccgtcc agctccaagc tttgtgtgcc
     4141 ctggaactct ccattttagg tcattgcttc agtttctttt ctaaaaaatt aggctgctgc
     4201 aaggtcagcc tgagaccact tctgccccga gaattctaga ctagtaagac ctggtgacat
     4261 acaaacgacg aagatatttt acaataatgc catggccctt gatagctaca cgaggtttgt
     4321 gttctgattt taaattaatg gatgacgtcg agactatatg gaggaaatga caaaggacag
     4381 gagagaggtg ggaaaggaag acctagactg aaaatggaag ttgggcagca gctccacgaa
     4441 agaaagacca gcccccaagt gcagtgacag cgcagagtag cgaccgagaa gttcccaggc
     4501 cagctgccac cccgccccca tgccattccc cagaacaggc acaggcgtta gggcggggcg
     4561 cgcgtgcgca gtcacgcgct gcgccaaccg ccacagctcc gggaaggcgg ccaggaccgg
     4621 ctagagccgg ttagaaccag tggcgcccgc ccacgagcca gcgcctcaca aagggagggc
     4681 ggctcacggc cctcgcgtat ccctgcgcgg cgctcgcgag ccgcccctcc cccggcgttt
     4741 gtccctgacg cagccccacc ggttgcgcag tccctccccg cccccgctct cccctccgca
     4801 gcctgcagcc cgagacttct gtaaaggact ggggccccgc aactgtcctc tcctgccctc
     4861 ttaagcgcag cgccatttta gcaacgcaga agcccggcgc cgggaagcct cagctcgcct
     4921 gaaggcaggt cccctctgac gcctccggga gcccaggttt cccagagtcc ttgggacgca
     4981 gcgacgagtt gtgctgctat cttagctgtc cttataggct ggccattcca ggtggtggta
     5041 tttagataaa accactcaaa ctctgcagtt tggtcttggg gtttggagga aagcttttat
     5101 ttttcttcct gctccggttc agaaggtctg aagctcatac ctaaccaggc ataacacaga
     5161 atctgcaaaa caaaaacccc taaaaaagca gacccagagc agtgtaaaca cttctgggtg
     5221 tgtccctgac tggctgccca aggtctctgt gtcttcggag acaaagccat tcgcttagtt
     5281 ggtctacttt aaaaggccac ttgaactcgc tttccatggc gatttgcctt gtgagcactt
     5341 tcaggagagc ctggaagctg aaaaacggta gaaaaatttc cgtgcgggcc gtggggggct
     5401 ggcggcaact ggggggccgc agatcagagt gggccactgg cagccaacgg cccccggggc
     5461 tcaggcgggg agcagctctg tggtgtggga ttgaggcgtt ttccaagagt gggttttcac
     5521 gtttctaaga tttcccaagc agacagcccg tgctgctccg atttctcgaa caaaaaagca
     5581 aaacgtgtgg ctgtcttggg agcaagtcgc aggactgcaa gcagttgggg gagaaagtcc
     5641 gccattttgc cacttctcaa ccgtccctgc aaggctgggg ctcagttgcg taatggaaag
     5701 taaagccctg aactatcaca ctttaatctt ccttcaaaag gtggtaaact atacctactg
     5761 tccctcaaga gaacacaaga agtgctttaa gaggtatttt aaaagttccg ggggttttgt
     5821 gaggcgtttg atgacccgtt taaaatatga tttccatgtt tcttttgtct aaagtttgca
     5881 gctcaaatct ttccacacgc tagtaattta agtatttctg catgtgtagt ttgcattcaa
     5941 gttccataag ctgttaagaa aaatctagaa aagtaaaact agaacctatt tttaaccgaa
     6001 gaactacttt ttgcctccct cacaaaggcg gcggaaggtg atcgaattcc ggtgatgcga
     6061 gttgttctcc gtctataaat acgcctcgcc cgagctgtgc ggtaggcatt gaggcagcca
     6121 gcgcaggggc ttctgctgag ggggcaggcg gagcttgagg aaaccgcaga taagtttttt
     6181 tctctttgaa agatagagat taatacaact acttaaaaaa tatagtcaat aggttactaa
     6241 gatattgctt agcgttaagt ttttaacgta attttaatag cttaagattt taagagaaaa
     6301 tatgaagact tagaagagta gcatgaggaa ggaaaagata aaaggtttct aaaacatgac
     6361 ggaggttgag atgaagcttc ttcatggagt aaaaaatgta tttaaaagaa aattgagaga
     6421 aaggactaca gagccccgaa ttaataccaa tagaagggca atgcttttag attaaaatga
     6481 aggtgactta aacagcttaa agtttagttt aaaagttgta ggtgattaaa ataatttgaa
     6541 ggcgatcttt taaaaagaga ttaaaccgaa ggtgattaaa agaccttgaa atccatgacg
     6601 cagggagaat tgcgtcattt aaagcctagt taacgcattt actaaacgca gacgaaaatg
     6661 gaaagattaa ttgggagtgg taggatgaaa caatttggag aagatagaag tttgaagtgg
     6721 aaaactggaa gacagaagta cgggaaggcg aagaaaagaa tagagaagat agggaaatta
     6781 gaagataaaa acatactttt agaagaaaaa agataaattt aaacctgaaa agtaggaagc
     6841 agaagaaaaa agacaagcta ggaaacaaaa agctaagggc aaaatgtaca aacttagaag
     6901 aaaattggaa gatagaaaca agatagaaaa tgaaaatatt gtcaagagtt tcagatagaa
     6961 aatgaaaaac aagctaagac aagtattgga gaagtataga agatagaaaa atataaagcc
     7021 aaaaattgga taaaatagca ctgaaaaaat gaggaaatta ttggtaacca atttatttta
     7081 aaagcccatc aatttaattt ctggtggtgc agaagttaga aggtaaagct tgagaagatg
     7141 agggtgttta cgtagaccag aaccaattta gaagaatact tgaagctaga aggggaagtt
     7201 ggttaaaaat cacatcaaaa agctactaaa aggactggtg taatttaaaa aaaactaagg
     7261 cagaaggctt ttggaagagt tagaagaatt tggaaggcct taaatatagt agcttagttt
     7321 gaaaaatgtg aaggactttc gtaacggaag taattcaaga tcaagagtaa ttaccaactt
     7381 aatgtttttg cattggactt tgagttaaga ttatttttta aatcctgagg actagcatta
     7441 attgacagct gacccaggtg ctacacagaa gtggattcag tgaatctagg aagacagcag
     7501 cagacaggat tccaggaacc agtgtttgat gaagctagga ctgaggagca agcgagcaag
     7561 cagcagttcg tggtgaagat aggaaaagag tccaggagcc agtgcgattt ggtgaaggaa
     7621 gctaggaaga aggaaggagc gctaacgatt tggtggtgaa gctaggaaaa aggattccag
     7681 gaaggagcga gtgcaatttg gtgatgaagg tagcaggcgg cttggcttgg caaccacacg
     7741 gaggaggcga gcaggcgttg tgcgtagagg atcctagacc agcatgccag tgtgccaagg
     7801 ccacagggaa agcgagtggt tggtaaaaat ccgtgaggtc ggcaatatgt tgtttttctg
     7861 gaacttactt atggtaacct tttatttatt ttctaatata atgggggagt ttcgtactga
     7921 ggtgtaaagg gatttatatg gggacgtagg ccgatttccg ggtgttgtag gtttctcttt
     7981 ttcaggctta tactcatgaa tcttgtctga agcttttgag ggcagactgc caagtcctgg
     8041 agaaatagta gatggcaagt ttgtgggttt ttttttttta cacgaatttg aggaaaacca
     8101 aatgaatttg atagccaaat tgagacaatt tcagcaaatc tgtaagcagt ttgtatgttt
     8161 agttggggta atgaagtatt tcagttttgt gaatagatga cctgttttta cttcctcacc
     8221 ctgaattcgt tttgtaaatg tagagtttgg atgtgtaact gaggcggggg ggagttttca
     8281 gtattttttt ttgtgggggt gggggcaaaa tatgttttca gttctttttc ccttaggtct
     8341 gtctagaatc ctaaaggcaa atgactcaag gtgtaacaga aaacaagaaa atccaatatc
     8401 aggataatca gaccaccaca ggtttacagt ttatagaaac tagagcagtt ctcacgttga
     8461 ggtctgtgga agagatgtcc attggagaaa tggctggtag ttactctttt ttccccccac
     8521 ccccttaatc agactttaaa agtgcttaac cccttaaact tgttattttt tacttgaagc
     8581 attttgggat ggtcttaaca gggaagagag agggtggggg agaaaatgtt tttttctaag
     8641 attttccaca gatgctatag tactattgac aaactgggtt agagaaggag tgtaccgctg
     8701 tgctgttggc acgaacacct tcagggactg gagctgcttt tatccttgga agagtattcc
     8761 cagttgaagc tgaaaagtac agcacagtgc agctttggtt catattcagt catctcagga
     8821 gaacttcaga agagcttgag taggccaaat gttgaagtta agttttccaa taatgtgact
     8881 tcttaaaagt tttattaaag gggaggggca aatattggca attagttggc agtggcctgt
     8941 tacggttggg attggtgggg tgggtttagg taattgttta gtttatgatt gcagataaac
     9001 tcatgccaga gaacttaaag tcttagaatg gaaaaagtaa agaaatatca acttccaagt
     9061 tggcaagtaa ctcccaatga tttagttttt ttccccccag tttgaattgg gaagctgggg
     9121 gaagttaaat atgagccact gggtgtacca gtgcattaat ttgggcaagg aaagtgtcat
     9181 aatttgatac tgtatctgtt ttccttcaaa gtatagagct tttggggaag gaaagtattg
     9241 aactgggggt tggtctggcc tactgggctg acattaacta caattatggg aaatgcaaaa
     9301 gttgtttgga tatggtagtg tgtggttctc ttttggaatt tttttcaggt gatttaataa
     9361 taatttaaaa ctactataga aactgcagag caaaggaagt ggcttaatga tcctgaaggg
     9421 atttcttctg atggtagctt ttgtattatc aagtaagatt ctattttcag ttgtgtgtaa
     9481 gcaagttttt tttttagtgt aggagaaata cttttccatt gtttaactgc aaaacaagat
     9541 gttaaggtat gcttcaaaaa ttttgtaaat tgtttatttt aaacttatct gtttgtaaat
     9601 tgtaactgat taagaattgt gatagttcag cttgaatgtc tcttagaggg tgggcttttg
     9661 ttgatgaggg aggggaaact tttttttttc tatagacttt tttcagataa catcttctga
     9721 gtcataacca gcctggcagt atgatggcct agatgcagag aaaacagctc cttggtgaat
     9781 tgataagtaa aggcagaaaa gattatatgt catacctcca ttggggaata agcataaccc
     9841 tgagattctt actactgatg agaacattat ctgcatatgc caaaaaattt taagcaaatg
     9901 aaagctacca atttaaagtt acggaatcta ccattttaaa gttaattgct tgtcaagcta
     9961 taaccacaaa aataatgaat tgatgagaaa tacaatgaag aggcaatgtc catctcaaaa
    10021 tactgctttt acaaaagcag aataaaagcg aaaagaaatg aaaatgttac actacattaa
    10081 tcctggaata aaagaagccg aaataaatga gagatgagtt gggatcaagt ggattgagga
    10141 ggctgtgctg tgtgccaatg tttcgtttgc ctcagacagg tatctcttcg ttatcagaag
    10201 agttgcttca tttcatctgg gagcagaaaa cagcaggcag ctgttaacag ataagtttaa
    10261 cttgcatctg cagtattgca tgttagggat aagtgcttat ttttaagagc tgtggagttc
    10321 ttaaatatca accatggcac tttctcctga ccccttccct aggggatttc aggattgaga
    10381 aatttttcca tcgagccttt ttaaaattgt aggacttgtt cctgtgggct tcagtgatgg
    10441 gatagtacac ttcactcaga ggcatttgca tctttaaata atttcttaaa agcctctaaa
    10501 gtgatcagtg ccttgatgcc aactaaggaa atttgtttag cattgaatct ctgaaggctc
    10561 tatgaaagga atagcatgat gtgctgttag aatcagatgt tactgctaaa atttacatgt
    10621 tgtgatgtaa attgtgtaga aaaccattaa atcattcaaa ataataaact atttttatta
    10681 gagaatgtat acttttagaa agctgtctcc ttatttaaat aaaatagtgt ttgtctgtag
    10741 ttcagtgttg gggcaatctt gggggggatt cttctctaat ctttcagaaa ctttgtctgc
    10801 gaacactctt taatggacca gatcaggatt tgagcggaag aacgaatgta actttaaggc
    10861 aggaaagaca aattttattc ttcataaagt gatgagcata taataattcc aggcacatgg
    10921 caatagaggc cctctaaata aggaataaat aacctcttag acaggtggga gattatgatc
    10981 agagtaaaag gtaattacac attttatttc cagaaagtca ggggtctata aattgacagt
    11041 gattagagta atactttttc acatttccaa agtttgcatg ttaactttaa atgcttacaa
    11101 tcttagagtg gtaggcaatg ttttacacta ttgaccttat atagggaagg gagggggtgc
    11161 ctgtggggtt ttaaagaatt ttcctttgca gaggcatttc atccttcatg aagccattca
    11221 ggattttgaa ttgcatatga gtgcttggct cttccttctg ttctagtgag tgtatgagac
    11281 cttgcagtga gtttatcagc atactcaaaa tttttttcct ggaatttgga gggatgggag
    11341 gagggggtgg ggcttacttg ttgtagcttt tttttttttt acagacttca cagagaatgc
    11401 agttgtcttg acttcaggtc tgtctgttct gttggcaagt aaatgcagta ctgttctgat
    11461 cccgctgcta ttagaatgca ttgtgaaacg actggagtat gattaaaagt tgtgttcccc
    11521 aatgcttgga gtagtgattg ttgaaggaaa aaatccagct gagtgataaa ggctgagtgt
    11581 tgaggaaatt tctgcagttt taagcagtcg tatttgtgat tgaagctgag tacattttgc
    11641 tggtgtattt ttaggtaaaa tgctttttgt tcatttctgg tggtgggagg ggactgaagc
    11701 ctttagtctt ttccagatgc aaccttaaaa tcagtgacaa gaaacattcc aaacaagcaa
    11761 cagtcttcaa gaaattaaac tggcaagtgg aaatgtttaa acagttcagt gatctttagt
    11821 gcattgttta tgtgtgggtt tctctctccc ctcccttggt cttaattctt acatgcagga
    11881 acactcagca gacacacgta tgcgaagggc cagagaagcc agacccagta agaaaaaata
    11941 gcctatttac tttaaataaa ccaaacattc cattttaaat gtggggattg ggaaccacta
    12001 gttctttcag atggtattct tcagactata gaaggagctt ccagttgaat tcaccagtgg
    12061 acaaaatgag gaaaacaggt gaacaagctt tttctgtatt tacatacaaa gtcagatcag
    12121 ttatgggaca atagtattga atagatttca gctttatgct ggagtaactg gcatgtgagc
    12181 aaactgtgtt ggcgtggggg tggaggggtg aggtgggcgc taagcctttt tttaagattt
    12241 ttcaggtacc cctcactaaa ggcaccgaag gcttaaagta ggacaaccat ggagccttcc
    12301 tgtggcagga gagacaacaa agcgctatta tcctaaggtc aagagaagtg tcagcctcac
    12361 ctgattttta ttagtaatga ggacttgcct caactccctc tttctggagt gaagcatccg
    12421 aaggaatgct tgaagtaccc ctgggcttct cttaacattt aagcaagctg tttttatagc
    12481 agctcttaat aataaagccc aaatctcaag cggtgcttga aggggaggga aagggggaaa
    12541 gcgggcaacc acttttccct agcttttcca gaagcctgtt aaaagcaagg tctccccaca
    12601 agcaacttct ctgccacatc gccaccccgt gccttttgat ctagcacaga cccttcaccc
    12661 ctcacctcga tgcagccagt agcttggatc cttgtgggca tgatccataa tcggtttcaa
    12721 ggtaacgatg gtgtcgaggt ctttggtggg ttgaactatg ttagaaaagg ccattaattt
    12781 gcctgcaaat tgttaacaga agggtattaa aaccacagct aagtagctct attataatac
    12841 ttatccagtg actaaaacca acttaaacca gtaagtggag aaataacatg ttcaagaact
    12901 gtaatgctgg gtgggaacat gtaacttgta gactggagaa gataggcatt tgagtggctg
    12961 agagggcttt tgggtgggaa tgcaaaaatt ctctgctaag actttttcag gtgaacataa
    13021 cagacttggc caagctagcc gctcgacgta atcttagcgg aagctgatct ccaatgctct
    13081 tcagtagggt catgaaggtt tttcttttcc tgagaaaaca acacgtattg ttttctcagg
    13141 ttttgctttt tggccttttt ctagcttaaa aaaaaaaaaa ggaaaagatg ctggtggttg
    13201 gcactcctgg tttccaggac ggggttcaaa tccctgcggc gtctttgctt tgactactaa
    13261 tctgtcttca ggactctttc tgtatttctc cttttctctg caggtgctag ttcttggagt
    13321 tttggggagg tgggaggtaa cagcacaata tctttgaact atatacatcc ttgatgtata
    13381 atttgtcagg agcttgactt gattgtatat tcatatttac acgagaacct aatataactg
    13441 ccttgtcttt ttcaggtaat agcctgcagc tggtgttttg agaagcccta ctgctgaaaa
    13501 cttaacaatt ttgtgtaata aaaatggaga agctctaaat tgttgtggtt cttttgtgaa
    13561 taaaaaaatc ttgattgggg aaaaaagatg ggtgttctgt gggcttgttc tgttaaatct
    13621 gtggtctata aacacagcac ccataattac agcataatct tcaagtaggg tacggacttt
    13681 gggggattgg tgcgagggta gtgggtgagt ggcctactaa aaagcccagt aacccccaca
    13741 ggaaaatagg gaacttcttt ttaagtagcc tcctttccac tatttagtaa ttggctgtga
    13801 gctgggctgg gggagaaatg gggcggggtg tgtgtgtcat tggaaagctc tcttttttgt
    13861 ttttttgaga cagtctcact ttgtccccca ggctggagtg tagtggcatg atctctgcaa
    13921 actgcaacct ccacttctgg ggtccaagtg gttgtcctgc ttcaccctcc ctgtagctgg
    13981 gactacaggt gcacaccacc acgcctggct aatttttgta ttttcagtta gagacgtggt
    14041 tttaccatat tggccaggct ggtctcaaac tcctgacctc gtgtgatcca cccgcctggg
    14101 cctctgaaag tgctgggatt acaggtgtga gccaccaagc ctggccgatc cttttaagtt
    14161 tttaaaccag ttaagctctt tggttccccc tcagagtccc aaggtcctgg gtcactcagg
    14221 atcacatttt cctttaatca gttgtcactg gtcccctttt gtccctttga acgtgctgtg
    14281 ggattagtag cagcatctgg ctgttggaag gactggctgg gatctcaggt gaaataccct
    14341 ccctggccct ccttaccctt aagatctccc gatccgtcga cgtcaggtgg cacttttcgg
    14401 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tac
//
