LOCUS       sequence-479176-        9612 bp    DNA     circular SYN 01-AUG-2025
DEFINITION  Dox-inducible expression of LATeNT in the mammalian cytosol; 
            lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9612)
  AUTHORS   Roh H, Kim D, Kim B, Jeon Y, Kim Y, Jacko M, Xu F, Lin C, Won Um J, 
            Ting AY
  TITLE     Light-activated tetanus neurotoxin for conditional proteolysis and 
            inducible synaptic inhibition in vivo
  JOURNAL   bioRxiv 2025.01.27.635161; doi: 10.1101/2025.01.27.635161
REFERENCE   2  (bases 1 to 9612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 1, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.01.27.635161; doi: 10.1101/2025.01.27.635161"
COMMENT     Sequence Label: pTRE3G-LATeNT
FEATURES             Location/Qualifiers
     source          1..9612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1752..1869
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1927..1945
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1963..1981
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1999..2017
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2035..2053
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2071..2089
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2107..2125
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2143..2161
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2206..2230
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2308..2317
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2317..2358
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4223..4811
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4276..4296)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4694..4705)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4831..5341
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5260..5278
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5351..6094
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             6104..6160
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6161..6757
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6654..6674
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6856..7089
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7161..7282
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7198..7217)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7252..7271
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7322..7457
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7457..7528
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7529..7597,7598..8389)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7747..7766)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8560..9148
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9049..9068
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9302..9319
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9436..9457
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9472..9477,9478..9495,9496..9502)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9510..9526
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9515..9537
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9534..9550
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9534..9550
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9569..9589
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9571..9589
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga caattctcga cctcgagaca aatggcagta
     1741 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     1801 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     1861 caaaattttc gggtttatta cagggacagc agagatccac tttggccgcg aatcgatatg
     1921 ttttactccc tatcagtgat agagaacgta tgaagagttt actccctatc agtgatagag
     1981 aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt ttactcccta
     2041 tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa cgtatctaca
     2101 gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc agtgatagag
     2161 aacgtataag ctttaggcgt gtacggtggg cgcctataaa agcagagctc gtttagtgaa
     2221 ccgtcagatc gcctggagca attccacaac acttttgtct tataccaact ttccgtacca
     2281 cttcctaccc tcgtaaatag agctagcgcc accatgggca agcccatccc caaccccctg
     2341 ctgggcctgg acagcaccaa gggctcgggc tcgacctcgg gctcgggcac cggtccgatc
     2401 accatcaaca acttccgtta ctccgacccg gttaacaacg acaccatcat catgatggaa
     2461 ccgccgtact gcaaaggtct agacatctac tacagagcct tcaaaatcac cgaccgtatc
     2521 tggatcgttc cggaacgtta cgaatttggt accaaaccgg aagacttcaa cccgccgtcc
     2581 tccctgatcg aaggtgcttc cgaatactac gacccgaact acctgcgtac cgactccgac
     2641 aaagaccgtt tcctgcagac catggttaaa ctgttcaacc gtatcaaaaa caacgttgct
     2701 ggtgaagctc tgctggacaa aatcatcaac gctatcccgt acctgggtaa ctcctactcc
     2761 ctgctggaca aattcgacac caactccaac tccgtttcct tcaacctgct ggaacaggat
     2821 ccgtccggtg ctaccaccaa atccgctatg ctgaccaacc tgatcatctt cggtccgggt
     2881 ccggttctga acaaaaacga agttcgtggt atcgttctgc gtgttgacaa caaaaactac
     2941 ttcccgtgcc gtgacggttt cggttccatc atgcagatgg ctttctgccc ggaatacgta
     3001 ccgaccttcg acaacgttat cgaaaacatc acctccctga ccatcggtaa atccaaatac
     3061 ttccaggacc cggctctgct gctgatgcac gaactgatcc acgttctgca cggtctgtac
     3121 ggcatgcagg tttcctccca cgaaatcatc ccgtccaaac aggaaatcta catgcagcac
     3181 acctacccga tctccgctga agagctcttc accttcggtg gtcaggacgc taacctgatc
     3241 tccatcgata tcaaaaacga cctgtacgaa aaaaccctga acgactacaa agctatcgct
     3301 aacaaactgt cccaggttac ctcctgcaac gacccgaaca tcgacatcga ctcctacaaa
     3361 cagatctatc agcagaagta tcagtttgat aaggattcca acggccagta tatagtgaac
     3421 gaggataagt ttcagatact gtataactcc ataatgtatg gcgggagcgg cagcgggggg
     3481 tccggtagcg gggagtttcg ggcaaccaca ctggaacgga tcgagaaaag cttcgtgatt
     3541 actgatccga gactgcctga caacccaatc atttttgtga gcaattcctt cctgcagctg
     3601 acagaatatt ctcgggaaga gatcctgggg cgcaattgcc gttttctgca gggacccgag
     3661 acagaccgtg ccactgttcg gaaaatcaga gatgctattg acaaccagac tgaagtgacc
     3721 gttcagctga tcaattatac caagagcggc aagaagttct ggaacgtgtt ccacctgcag
     3781 ccgatgcgcg attataaggg cgacgtccag tacttcattg gcgtgcagct ggatggcacc
     3841 gaacgccttc atggcgccgc tgagcgtgag gcggtctgcc tggtcaaaaa gacagccttt
     3901 cagattttta ctgagataga gctgggcaag aagtttaaca taaagactcg cttaagctat
     3961 ttttccatga accacgatcc tgtgaagata cctaacctgc tggatgatac tatatataac
     4021 gatactgagg gctttaacat agagtccaag gatctgaagt ccgagtataa gggccagaac
     4081 atgagggtga acactaacgc ctttaggaac gtcgacggtt ccggtctggt ttccaaactg
     4141 atcggtctgt gcaaaaaaat catcccgccg accaacatcc gtgaaaacct gtacaaccgt
     4201 accgcttaat agggccgaat tcaatcaacc tctggattac aaaatttgtg aaagattgac
     4261 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     4321 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     4381 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     4441 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     4501 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     4561 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     4621 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     4681 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     4741 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     4801 cgcctccccg cctgtctgca gatatcatcc ggggttgggg ttgcgccttt tccaaggcag
     4861 ccctgggttt gcgcagggac gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc
     4921 cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc
     4981 tacccttgtg ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt
     5041 gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc
     5101 agacggacag cgccagggag caatggcagc gcgccgaccg cgatgggctg tggccaatag
     5161 cggctgctca gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc gggaggcggg
     5221 gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc
     5281 tccggagcgc acgtcggcag tcggctccct cgttgaccga atcaccgacc tctctcccca
     5341 gcaattcacc atgtctagac tggacaagag caaagtcata aactctgctc tggaattact
     5401 caatggagtc ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc tgggagttga
     5461 gcagcctacc ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat
     5521 cgagatgctg gacaggcatc atacccactc ctgccccctg gaaggcgagt catggcaaga
     5581 ctttctgcgg aacaacgcca agtcataccg ctgtgctctc ctctcacatc gcgacggggc
     5641 taaagtgcat ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg aaaatcagct
     5701 cgcgttcctg tgtcagcaag gcttctccct ggagaacgca ctgtacgctc tgtccgccgt
     5761 gggccacttt acactgggct gcgtattgga ggaacaggag catcaagtag caaaagagga
     5821 aagagagaca cctaccaccg attctatgcc cccacttctg aaacaagcaa ttgagctgtt
     5881 cgaccggcag ggagccgaac ctgccttcct tttcggcctg gaactaatca tatgtggcct
     5941 ggagaaacag ctaaagtgcg aaagcggcgg gccgaccgac gcccttgacg attttgactt
     6001 agacatgctc ccagccgatg cccttgacga ctttgacctt gatatgctgc ctgctgacgc
     6061 tcttgacgat tttgaccttg acatgctccc cgggggaagc ggagctacta acttcagcct
     6121 gctgaagcag gctggagacg tggaggagaa ccctggacct accgagtaca agcccacggt
     6181 gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc
     6241 cgactacccc gccacgcgcc acaccgtcga cccggaccgc cacatcgagc gggtcaccga
     6301 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     6361 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     6421 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     6481 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     6541 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     6601 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     6661 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     6721 gcgcacctgg tgcatgaccc gcaagcccgg tgcctaactg tttcgcctcg gcgtccggac
     6781 tagaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     6841 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     6901 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     6961 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     7021 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     7081 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     7141 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     7201 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     7261 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     7321 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     7381 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     7441 aggctttttt ggaggccttt caaatatgta tccgctcatg agacaataac cctgataaat
     7501 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     7561 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     7621 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     7681 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     7741 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     7801 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     7861 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     7921 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     7981 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     8041 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     8101 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     8161 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     8221 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     8281 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     8341 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     8401 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     8461 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     8521 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     8581 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     8641 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     8701 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     8761 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     8821 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     8881 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     8941 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     9001 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     9061 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     9121 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     9181 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     9241 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     9301 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     9361 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     9421 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     9481 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     9541 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
     9601 ggagctgcaa gc
//
