LOCUS       sequence-479182-        2203 bp    DNA     circular SYN 07-AUG-2025
DEFINITION  t0 terminator part plasmid for Golden Gate Assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2203)
  TITLE     Murray Lab Golden Gate Plasmids 2025
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: eEMLP01
FEATURES             Location/Qualifiers
     source          1..2203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(8..799,800..868))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     631..650
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(869..973)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1041..1059
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1063..1106)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1109..1130
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1149..1243
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     misc_feature    1255..1275
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(1281..1298)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1452..2040)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1532..1551)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
       61 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
      121 ctggccccag tgctgcaatg ataccgcgcg acccacgctc accggctcca gatttatcag
      181 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
      241 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
      301 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
      361 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
      421 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
      481 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
      541 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
      601 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
      661 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
      721 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
      781 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
      841 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
      901 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
      961 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     1021 tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat
     1081 ccttagcttt cgctaaggat gatttctgga attcgcggcc gcttctagag actagtgggt
     1141 ctcaaggtga ctcctgttga tagatccagt aatgacctca gaactccatc tggatttgtt
     1201 cagaacgctc ggttgccgcc gggcgttttt tattggtgag aatgcttaga gacctactag
     1261 tagcggccgc tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     1321 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     1381 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     1441 ttgctggcgt ttttccacag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     1501 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     1561 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     1621 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     1681 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     1741 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     1801 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     1861 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     1921 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     1981 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     2041 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     2101 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     2161 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggt
//
