LOCUS       sequence-479184-        8394 bp    DNA     circular SYN 07-AUG-2025
DEFINITION  phenazine gene for Golden Gate Assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8394)
  TITLE     Murray Lab Golden Gate Plasmids 2025
REFERENCE   2  (bases 1 to 8394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: eOYWP02
FEATURES             Location/Qualifiers
     source          1..8394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3707..3718
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    6302..6322
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(6328..6345)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6499..7087)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6579..6598)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7258..8049,8050..8118))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7881..7900
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8119..8223)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8291..8309
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(8313..8356)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    8359..8380
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatgcccggt tcgctttcat ctggcggctt taatgaccat cttgaacttc gccgaaaaaa
       61 ccgcgccacg gttgatcagt acatgcgcac caacggcgaa gatcgcctgc gccgccatga
      121 gttattcacg ccggatggca gtggcggctc ctggaacacc gaaaccggtg aaccccttgt
      181 ctttaagggg cacgcgaagc tggccgcact cggcgtatgg ctgcaccagt gcttcccgga
      241 ctggcaatgg cacaacgtcc gggtgtttga aaccgacaac cccaaccact tctgggtgga
      301 aagtgacggc cgcggcacca ctcgagttcc tggttacccg gaaggttatt gcgaaaacca
      361 ttacatccac tccttcgagt tggacaacgg caagatcacg caaaaccgtg aattcatgaa
      421 cccgtttgaa caacttcgcg ccttgggcat cccggtgcca aaaatcaagc gtgaaggtat
      481 ccccgcctca taagtccttt atccattgga gattgaacat gcctgatagc acagtgcaac
      541 aacccatcac tgacgatacg gaactacgcc gcaagaatcg cgccacggtt gaacaataca
      601 tgcgcaccaa aggccaggac cgcctgcgcc ggcatgaact gtttaccgaa gacggttcag
      661 gcggcttatg gaccaccgac acgggtgcgc cgattgtaat cagtggcaaa gccaagttgg
      721 ccgaacatgc tgtttggtca ctcaagtgct tcccggattg ggaatggtac aacgtcaagg
      781 tatttgaaac cgatgacccc aaccatatct gggtcgagtg tgatggccac ggcaagattc
      841 tctttcctgg ttacccggag ggttattacg aaaaccactt cctgcactcc ttcgagctgc
      901 aagacggcaa agtaaaacgc aatcgcgaat tcatgaatgt ctttcaacaa ttgcgcgccc
      961 tgggtattcc agtgccgcac atcaagcgtg aaggcattcc tgcctgatcc ttttgggaga
     1021 gcaatcgcat catggaagac ttactgaaac gggttctgaa gtgtgaagcg ttgcagcagc
     1081 ctcaatggag cgaaccctcg caattgcatg acgcgcaggc ttacctcagg gacagcgcgt
     1141 cactgattcg tgtcgaagat atcctggttt tgcgcgccac cctggccagg gtcgcggctg
     1201 gcgaagcgat gatcatccag tgcggcgact gcgccgagga catggatgaa agcgctgccg
     1261 accatgtgac ccgcaaagcc gcgctgctgg atatgctggc cgggacgttc aggctggtca
     1321 cccagcaacc ggtggtgcgg gtggggcgaa tcgccgggca gtttgccaag cctcgctcca
     1381 accacagcga acgcatcggc gatgtcgagt tgccggtgta ccgcggcgac atggtcaacg
     1441 gtcgcgacgc tgtcctcggc catcgccagc atgatgcgca acgcttggta cgaggctatc
     1501 gcgccgccca ggacatcatg caacacctgg gatggaagga accgtccggc caggagcagc
     1561 tcaccggctc ccctgcctgg accagtcacg agatgctggt gctcgactac gaactgccgc
     1621 aagtacgccg ggacgaacag ggccgcacat ttctcggttc tacccactgg ccgtggattg
     1681 gcgagcgtac ccgtcagttg acgggcgctc acgtagcgct gctcagcgaa gtgctcaacc
     1741 cggtggcctg caaggtcggc ccggatatca cccaggatca gttactgagc ctgtgcgagc
     1801 gcctggaccc caggcgcgaa cccggccggc tgaccctgat tgcccgcatg ggcgcccaca
     1861 aggtggccga ccgcctgcca ccgctggttg aagcggtgcg ccgtgccggc cataagatca
     1921 tctggctcag cgacccgatg cacggcaata ccatcgtcgc gccctgcggc aataagaccc
     1981 gcatggtgca gaccatcacc gacgaaatca ccgccttcaa acatgcggtt gtctcggccg
     2041 gtggcgtggc cggcggcctg catctggaaa ccactcccga tgacgtcagc gagtgcgcct
     2101 ccgatgccgc cggcctgggc caggttggca gccactacaa aagcctgtgc gacccgcgcc
     2161 tgaacccctg gcaagccatt actgcggtga tggcctggaa agcctgccct cctccctctt
     2221 ttgtttccct ttgactggag tttgtcgcca tgaccggcat tccatcgatc gtcccttacg
     2281 ctctgcctac ttctcgcgac ctgcccgcca acctcgcgca atggcacatc gaccccgaac
     2341 gcgccgtact gttggtgcat gacatgcagc gctacttcct gcggcccttg ccggatgcct
     2401 tgcgtgacca agtggtgggt aatgccgcac gcattcgcca gtgggctgcc gacaacggcg
     2461 tgccagtggc ctacaccgcc cagccgggca gcatgaacga ggaacaacgc gggctgctca
     2521 aggacttctg gggcccgggc atgaaggcca gcccgaccga ccgtgaggtc gtcgacgccc
     2581 tcgcccccca gcccggtgac tggctgctga ccaagtggcg ctacagcgcg ttcttcaact
     2641 ccgacttgtt gcaacgcctg cacgccagcg ggcgcgatca gttgatcctg tgtggggtgt
     2701 atgcccatgt cggggtattg atttccagcg tggatgcgta ctccaacgat atccaaccct
     2761 tcctcgttgc cgacgcgatt gccgacttca gcaaggagca ccactggatg gccatggaat
     2821 acgccgccag ccgttgcgcc atggtcatca ccaccgatga ggtggtgcta tgagccaagc
     2881 cgccgcccgc ctcatggaac gcatcctgca accggtaccc gaaccctttg cgctcttgta
     2941 tcgcccggag tccagcggcc ccgggctgct gaatgtgctg attggcgaga tgtcgcagcc
     3001 gcaggtcctg gccgatatcg acctgcccgc cccctcaatc ggtgcccctc gcctggatgt
     3061 actgacgctg attccctact gccagatcgc tgaacgcggc tttgcggcgg tggacgatca
     3121 gtcgccgctg ctggcgatga acattaccga gcagcaaacc atcagcatcg agcagatgct
     3181 ggcgttactg cccaacgtgc cgatccagtt gaataacgaa cgcttcgacc tcagcgacgc
     3241 cagctatgcc gagatcgtca gccaggtgat cgccaacgaa atcggttccg gggaaggcgc
     3301 caacttcgtg atcaagcgca ccttcctggc ggagatcagc gagtaccagc cggccagtgc
     3361 gctgtctttc tttcgtcatc tgctggaacg agagaaaggc gtctattgga cattcatcat
     3421 tcacaccggc agccgcacct tcgtcggtgc gtctcccgag cgccacatca gcgtcaagga
     3481 tgggcttgcg gtgatgaacc ccatcagcgg cacttaccgc taccctcctg ccggccccag
     3541 cctgacggag gtcatggact tcctggccga ccgcaaggaa gccgatgagc tgtatatggt
     3601 ggtcgatgaa gaactgaaaa tgatggcgcg tatttgtgaa gacggcggac acgtcctcgg
     3661 tccctacctc aaggaaatga cccatctggc gcacaccgaa tacttcatcg aaggcaggac
     3721 ccgccgcgat gtgcgcgaaa tcctgcacga aaccctgttc gccccgacgg tcaccggcag
     3781 cccgctggaa agcgcctgcc gggtgatcga gcgctatgag ccgcaaggca gggcgtacta
     3841 cagcggcatg gccgcgctga ttggcagcga tggcaagggc ggacgctccc tggactcggc
     3901 gattctgatt cgcaccgccg acatcgataa ctgcgggcag gttcggatca gtgtgggttc
     3961 gaccatcgtg cggcactccg aaccgctgac cgaagccgcc gaaagccggg ccaaggctgc
     4021 gggcctgatc gccgcgctga aaaaccaggc ggcctcgcgc ttcggcgacc acctgcaggt
     4081 gcgcgcggca ttggccagcc gcaatgccta cgtctcggat ttctggctga tgaacagcca
     4141 gcaacggcaa cagacccagt ccgacttcag cggccgccag gtgctgatcg tcgacgccga
     4201 agacacgttc acctcgatga tcgccaaaca gctgcgggct ctcgggctgg tggtgacggt
     4261 gcgcagtttc agtgacgagt acagtttcga cggttacgac ctggtgatca tgggccctgg
     4321 ccccggcaac ccgagcgatg tgcaactgcc gaaaatcgac catctgcatg tggccatccg
     4381 ttccctgctc aaccagcagc gaccgttcct cgcggtgtgc ctgagccatc aggtgttgag
     4441 cctgtgcctg ggcctggaat tgcaacgcaa ggccattccc aaccagggcg tgcaaaaaca
     4501 gatcgacctg ttcggcaacg ccgaacgagt aggtttctac aacacgttcg cggcgcgcag
     4561 ctccagtgac cgcctggaca tcgacggtat cggcaccgtg gaaatcagtc gcgacagcga
     4621 aaccggcgag gtacatgccc tgcgtgggcc ggcgtttgcc tccatgcagt ttcatgccga
     4681 gtcgcttttg acccaggaag gcccgcgcat catcgccgac ctgctgcggc acgccctcat
     4741 ccacacgcct gtcgacagca gcgtttcggc cgccgggaga taaccatgca caactacgtc
     4801 attatcgacg cctttgccag cgtcccgctg gaaggcaatc cggtcgcggt gttctttgac
     4861 gccgatgatt taccgcccgc gcaaatgcag cgcatcgccc gagaaatgaa cctctcggag
     4921 agcacttttg tgctcaagcc gcgcaacggt ggagatgcgc tgatccggat tttcacaccg
     4981 gtcaacgaac tgccttttgc cgggcacccg ttgctgggta cggccattgc cctgggagca
     5041 cataccgaca accaccggct gtatctggaa acccagatgg gcaccatcgc ctttgaactg
     5101 gagcgccaga acggcagcgt tatcgccgcc agcatggacc agccgatccc gacctggacg
     5161 gccctggggc gtgacgctga actgctcaag gccttgggca tcagtgattc gacctttccc
     5221 atcgagatct atcacaacgg cccgcgccat gtgtttgtcg gcctgccgag cattgacgcg
     5281 ttatcggccc tgcaccccga ccaccgtgca ctgtccaact tccatgacat ggcgatcaac
     5341 tgttttgccg gcgcaggacg acgctggcgc agccgcatgt tctcaccggc ctacggcgtt
     5401 gtggaggatg cggccacagg ctccgccgcc ggaccattgg cgattcatct ggcgcgccat
     5461 ggccagattg agtttggcca gccagtcgag attttacagg gtgtggaaat cggccgcccc
     5521 tcactgatgt tcgccaaagc tgagggccgc gccgagcaac tgacgcgagt cgaggtatca
     5581 ggtaatggcg ttacatttgg acgggggacc atcgtgctat gaacggctca atacaaggca
     5641 agccgctgtt gggtaaaggc atgtcggaat ccctcaccgg cacattggat gcgcccttcc
     5701 ccgaatatca gacactgccc gccgacccca tgagcgtgct gcacaattgg ctggagcgtg
     5761 cgcgccgcgt gggcatccgc gagcccaggg cgctggcgct ggccaccgcc gacagccagg
     5821 gccggccctc cacgcgtatc gtggtgatca gcgagatcag tgacgccggc gtggtgttca
     5881 gcacccatgc cggcagccag aaaggccgtg aactgctcca caacccctgg gcttcgggcg
     5941 tgctgtattg gcgcgaaacc agtcagcaaa tcatcctcaa tggccaggcc gtgcgcttgc
     6001 cgaacgccaa ggccgatgat gcctggctca agcgccctta tgccacgcac ccgatgtcat
     6061 cggtgtctcg ccagagtgaa gaactgcagg acgtgcaggc catgcgcaac gccgccaggc
     6121 aacttgccga gctgcagggt ccactgccgc gtccggaggg atattgcgtg ttcgagttgc
     6181 gccttgaatc gctggagttc tggggaaatg ggcaggagcg cttgcatgag cggttgcggt
     6241 atgaccgcag cgatacaggc tggaacgtgc ggcgtttgca gccctgaata aggtagagac
     6301 ctactagtag cggccgctgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg
     6361 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     6421 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     6481 ggccgcgttg ctggcgtttt tccacaggct ccgcccccct gacgagcatc acaaaaatcg
     6541 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     6601 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     6661 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     6721 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     6781 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     6841 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     6901 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     6961 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     7021 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     7081 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     7141 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     7201 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     7261 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     7321 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     7381 tgctgcaatg ataccgcgcg acccacgctc accggctcca gatttatcag caataaacca
     7441 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     7501 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     7561 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     7621 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     7681 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     7741 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     7801 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     7861 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     7921 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     7981 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     8041 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     8101 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     8161 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     8221 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     8281 aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt
     8341 cgctaaggat gatttctgga attcgcggcc gcttctagag actagtgggt ctca
//
