LOCUS       sequence-479249-       11276 bp    DNA     circular SYN 22-JUL-2025
DEFINITION  A segment of the T7 phage genome was inserted into the artificial 
            bacterial chromosome . The genome segment can be stably replicated 
            in plasmid and can be genetically modified at the plasmid level.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11276)
  AUTHORS   Zhang H, Zhu R, Wang Z, He R, Zhang Y, Luan J, Yan Y, Zhang Y, Wang 
            H
  TITLE     Programming virulent bacteriophages by developing a multiplex genome
            engineering method
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11276)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 22, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBAC-T7-F7
FEATURES             Location/Qualifiers
     source          1..11276
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1..30
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     primer_bind     3550..3569
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3550..3568
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             4541..4549
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      complement(5114..5141)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(5174..5833)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     5748..5767
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(5834..5936)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      6863..7082
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             7173..7928
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    7931..8181
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             8507..9682
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMFRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             9682..10653
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    10726..11199
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
ORIGIN
        1 ggcttacctt cacgggtggg cctttcttcg gccggcatgg ctgctcgcta aacaagagcg
       61 caacgggttc ccgtttgaca caaaagcaat cgaagagttg tacgtagagt tagctgctcg
      121 ccgctctgag ttgctccgta aattgaccga aacgttcggc tcgtggtatc agcctaaagg
      181 tggcactgag atgttctgcc atccgcgaac aggtaagcca ctacctaaat accctcgcat
      241 taagacacct aaagttggtg gtatctttaa gaagcctaag aacaaggcac agcgagaagg
      301 ccgtgagcct tgcgaacttg atacccgcga gtacgttgct ggtgctcctt acaccccagt
      361 tgaacatgtt gtgtttaacc cttcgtctcg tgaccacatt cagaagaaac tccaagaggc
      421 tgggtgggtc ccgaccaagt acaccgataa gggtgctcct gtggtggacg atgaggtact
      481 cgaaggagta cgtgtagatg accctgagaa gcaagccgct atcgacctca ttaaagagta
      541 cttgatgatt cagaagcgaa tcggacagtc tgctgaggga gacaaagcat ggcttcgtta
      601 tgttgctgag gatggtaaga ttcatggttc tgttaaccct aatggagcag ttacgggtcg
      661 tgcgacccat gcgttcccaa accttgcgca aattccgggt gtacgttctc cttatggaga
      721 gcagtgtcgc gctgcttttg gcgctgagca ccatttggat gggataactg gtaagccttg
      781 ggttcaggct ggcatcgacg catccggtct tgagctacgc tgcttggctc acttcatggc
      841 tcgctttgat aacggcgagt acgctcacga gattcttaac ggcgacatcc acactaagaa
      901 ccagatagct gctgaactac ctacccgaga taacgctaag acgttcatct atgggttcct
      961 ctatggtgct ggtgatgaga agattggaca gattgttggt gctggtaaag agcgcggtaa
     1021 ggaactcaag aagaaattcc ttgagaacac ccccgcgatt gcagcactcc gcgagtctat
     1081 ccaacagaca cttgtcgagt cctctcaatg ggtagctggt gagcaacaag tcaagtggaa
     1141 acgccgctgg attaaaggtc tggatggtcg taaggtacac gttcgtagtc ctcacgctgc
     1201 cttgaatacc ctactgcaat ctgctggtgc tctcatctgc aaactgtgga ttatcaagac
     1261 cgaagagatg ctcgtagaga aaggcttgaa gcatggctgg gatggggact ttgcgtacat
     1321 ggcatgggta catgatgaaa tccaagtagg ctgccgtacc gaagagattg ctcaggtggt
     1381 cattgagacc gcacaagaag cgatgcgctg ggttggagac cactggaact tccggtgtct
     1441 tctggatacc gaaggtaaga tgggtcctaa ttgggcgatt tgccactgat acaggaggct
     1501 actcatgaac gaaagacact taacaggtgc tgcttctgaa atgctagtag cctacaaatt
     1561 taccaaagct gggtacactg tctattaccc tatgctgact cagagtaaag aggacttggt
     1621 tgtatgtaag gatggtaaat ttagtaaggt tcaggttaaa acagccacaa cggttcaaac
     1681 caacacagga gatgccaagc aggttaggct aggtggatgc ggtaggtccg aatataagga
     1741 tggagacttt gacattcttg cggttgtggt tgacgaagat gtgcttattt tcacatggga
     1801 cgaagtaaaa ggtaagacat ccatgtgtgt cggcaagaga aacaaaggca taaaactata
     1861 ggagaaatta ttatggctat gacaaagaaa tttaaagtgt ccttcgacgt taccgcaaag
     1921 atgtcgtctg acgttcaggc aatcttagag aaagatatgc tgcatctatg taagcaggtc
     1981 ggctcaggtg cgattgtccc caatggtaaa cagaaggaaa tgattgtcca gttcctgaca
     2041 cacggtatgg aaggattgat gacattcgta gtacgtacat catttcgtga ggccattaag
     2101 gacatgcacg aagagtatgc agataaggac tctttcaaac aatctcctgc aacagtacgg
     2161 gaggtgttct gatgtctgac tacctgaaag tgctgcaagc aatcaaaagt tgccctaaga
     2221 ctttccagtc caactatgta cggaacaatg cgagcctcgt agcggaggcc gcttcccgtg
     2281 gtcacatctc gtgcctgact actagtggac gtaacggtgg cgcttgggaa atcactgctt
     2341 ccggtactcg ctttctgaaa cgaatgggag gatgtgtcta atgtctcgtg accttgtgac
     2401 tattccacgc gatgtgtgga acgatataca gggctacatc gactctctgg aacgtgagaa
     2461 cgatagcctt aagaatcaac taatggaagc tgacgaatac gtagcggaac tagaggagaa
     2521 acttaatggc acttcttgac cttaaacaat tctatgagtt acgtgaaggc tgcgacgaca
     2581 agggtatcct tgtgatggac ggcgactggc tggtcttcca agctatgagt gctgctgagt
     2641 ttgatgcctc ttgggaggaa gagatttggc accgatgctg tgaccacgct aaggcccgtc
     2701 agattcttga ggattccatt aagtcctacg agacccgtaa gaaggcttgg gcaggtgctc
     2761 caattgtcct tgcgttcacc gatagtgtta actggcgtaa agaactggtt gacccgaact
     2821 ataaggctaa ccgtaaggcc gtgaagaaac ctgtagggta ctttgagttc cttgatgctc
     2881 tctttgagcg cgaagagttc tattgcatcc gtgagcctat gcttgagggt gatgacgtta
     2941 tgggagttat tgcttccaat ccgtctgcct tcggtgctcg taaggctgta atcatctctt
     3001 gcgataagga ctttaagacc atccctaact gtgacttcct gtggtgtacc actggtaaca
     3061 tcctgactca gaccgaagag tccgctgact ggtggcacct cttccagacc atcaagggtg
     3121 acatcactga tggttactca gggattgctg gatggggtga taccgccgag gacttcttga
     3181 ataacccgtt cataaccgag cctaaaacgt ctgtgcttaa gtccggtaag aacaaaggcc
     3241 aagaggttac taaatgggtt aaacgcgacc ctgagcctca tgagacgctt tgggactgca
     3301 ttaagtccat tggcgcgaag gctggtatga ccgaagagga tattatcaag cagggccaaa
     3361 tggctcgaat cctacggttc aacgagtaca actttattga caaggagatt tacctgtgga
     3421 gaccgtagcg tatattggtc tgggtctttg tgttctcgga gtgtgcctca tttcgtgggg
     3481 cctttgggac ttagccagaa taatcaagtc gttacacgac actaagtgat aaactcaagg
     3541 tccctaaatt aatacgactc actataggga gataggggcc tttacgatta ttactttaag
     3601 atttaactct aagaggaatc tttattatgt taacacctat taaccaatta cttaagaacc
     3661 ctaacgatat tccagatgta cctcgtgcaa ccgctgagta tctacaggtt cgattcaact
     3721 atgcgtacct cgaagcgtct ggtcatatag gacttatgcg tgctaatggt tgtagtgagg
     3781 cccacatctt gggtttcatt cagggcctac agtatgcctc taacgtcatt gacgagattg
     3841 agttacgcaa ggaacaacta agagatgatg gggaggattg acactatgtg tttctcaccg
     3901 aaaattaaaa ctccgaagat ggataccaat cagattcgag ccgttgagcc agcgcctctg
     3961 acccaagaag tgtcaagcgt ggagttcggt gggtcttctg atgagacgga taccgagggc
     4021 accgaagtgt ctggacgcaa aggcctcaag gtcgaacgtg atgattccgt agcgaagtct
     4081 aaagccagcg gcaatggctc cgctcgtatg aaatcttcca tccgtaagtc cgcatttgga
     4141 ggtaagaagt gatgtctgag ttcacatgtg tggaggctaa gagtcgcttc cgtgcaatcc
     4201 ggtggactgt ggaacacctt gggttgccta aaggattcga aggacacttt gtgggctaca
     4261 gcctctacgt agacgaagtg atggacatgt ctggttgccg tgaagagtac attctggact
     4321 ctaccggaaa acatgtagcg tacttcgcgt ggtgcgtaag ctgtgacatt caccacaaag
     4381 gagacattct ggatgtaacg tccgttgtca ttaatcctga ggcagactct aagggcttac
     4441 agcgattcct agcgaaacgc tttaagtacc ttgcggaact ccacgattgc gattgggtgt
     4501 ctcgttgtaa gcatgaaggc gagacaatgc gtgtatactt taaggaggta taagttatgg
     4561 gtaagaaagt taagaaggcc gtgaagaaag tcaccaagtc cgttaagaaa gtcgttaagg
     4621 aaggggctcg tccggttaaa caggttgctg gcggtctagc tggtctggct ggtggtactg
     4681 gtgaagcaca gatggtggaa gcaccacaag ctgccgcaca gattgttgac gtacctgaga
     4741 aagaggtttc cactgaggac gaagcacaga cagaaagcgg acgcaagaaa gctcgtgctg
     4801 gcggtaagaa atccttgagt gtagcccgta gctccggtgg cggtatcaac atttaatcag
     4861 gaggttatcg tggaagactg cattgaatgg accggaggtg tcaactctaa gggttatggt
     4921 cgtaagtggg ttaatggtaa acttgtgact ccacataggc acatctatga ggagacatat
     4981 ggtccagttc caacaggaat tgtggtgatg catatctgcg ataaccctag gtgctataac
     5041 ataaagcacc ttacgcttgg aactccaaag gataattccg aggacatggt taccaaaggt
     5101 agacagggcc ggccgcagaa aggcccaccc gaaggtgagc cgtttaaggg caccaataac
     5161 tgccttaaaa aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
     5221 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
     5281 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
     5341 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
     5401 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
     5461 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
     5521 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     5581 atatcaccag ctcaccgtct ttcattgcca tacggaattc cggatgagca ttcatcaggc
     5641 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     5701 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     5761 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
     5821 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
     5881 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt
     5941 ctcattttcg ccaaaagttg gcccagggct tcccggtatc aacagggaca ccaggattta
     6001 tttattctgc gaagtgatct tccgtcacag gtatttattc gcgataagct catggagcgg
     6061 cgtaaccgtc gcacaggaag gacagagaaa gcgcggatct gggaagtgac ggacagaacg
     6121 gtcaggacct ggattgggga ggcggttgcc gccgctgctg ctgacggtgt gacgttctct
     6181 gttccggtca caccacatac gttccgccat tcctatgcga tgcacatgct gtatgccggt
     6241 ataccgctga aagttctgca aagcctgatg ggacataagt ccatcagttc aacggaagtc
     6301 tacacgaagg tttttgcgct ggatgtggct gcccggcacc gggtgcagtt tgcgatgccg
     6361 gagtctgatg cggttgcgat gctgaaacaa ttatcctgag aataaatgcc ttggccttta
     6421 tatggaaatg tggaactgag tggatatgct gtttttgtct gttaaacaga gaagctggct
     6481 gttatccact gagaagcgaa cgaaacagtc gggaaaatct cccattatcg tagagatccg
     6541 cattattaat ctcaggagcc tgtgtagcgt ttataggaag tagtgttctg tcatgatgcc
     6601 tgcaagcggt aacgaaaacg atttgaatat gccttcagga acaatagaaa tcttcgtgcg
     6661 gtgttacgtt gaagtggagc ggattatgtc agcaatggac agaacaacct aatgaacaca
     6721 gaaccatgat gtggtctgtc cttttacagc cagtagtgct cgccgcagtc gagcgacagg
     6781 gcgaagccct cgagtgagcg aggaagcacc agggaacagc acttatatat tctgcttaca
     6841 cacgatgcct gaaaaaactt cccttggggt tatccactta tccacgggga tatttttata
     6901 attatttttt ttatagtttt tagatcttct tttttagagc gccttgtagg cctttatcca
     6961 tgctggttct agagaaggtg ttgtgacaaa ttgccctttc agtgtgacaa atcaccctca
     7021 aatgacagtc ctgtctgtga caaattgccc ttaaccctgt gacaaattgc cctcagaaga
     7081 agctgttttt tcacaaagtt atccctgctt attgactctt ttttatttag tgtgacaatc
     7141 taaaaacttg tcacacttca catggatctg tcatggcgga aacagcggtt atcaatcaca
     7201 agaaacgtaa aaatagcccg cgaatcgtcc agtcaaacga cctcactgag gcggcatata
     7261 gtctctcccg ggatcaaaaa cgtatgctgt atctgttcgt tgaccagatc agaaaatctg
     7321 atggcaccct acaggaacat gacggtatct gcgagatcca tgttgctaaa tatgctgaaa
     7381 tattcggatt gacctctgcg gaagccagta aggatatacg gcaggcattg aagagtttcg
     7441 cggggaagga agtggttttt tatcgccctg aagaggatgc cggcgatgaa aaaggctatg
     7501 aatcttttcc ttggtttatc aaacgtgcgc acagtccatc cagagggctt tacagtgtac
     7561 atatcaaccc atatctcatt cccttcttta tcgggttaca gaaccggttt acgcagtttc
     7621 ggcttagtga aacaaaagaa atcaccaatc cgtatgccat gcgtttatac gaatccctgt
     7681 gtcagtatcg taagccggat ggctcaggca tcgtctctct gaaaatcgac tggatcatag
     7741 agcgttacca gctgcctcaa agttaccagc gtatgcctga cttccgccgc cgcttcctgc
     7801 aggtctgtgt taatgagatc aacagcagaa ctccaatgcg cctctcatac attgagaaaa
     7861 agaaaggccg ccagacgact catatcgtat tttccttccg cgatatcact tccatgacga
     7921 caggatagtc tgagggttat ctgtcacaga tttgagggtg gttcgtcaca tttgttctga
     7981 cctactgagg gtaatttgtc acagttttgc tgtttccttc agcctgcatg gattttctca
     8041 tactttttga actgtaattt ttaaggaagc caaatttgag ggcagtttgt cacagttgat
     8101 ttccttctct ttcccttcgt catgtgacct gatatcgggg gttagttcgt catcattgat
     8161 gagggttgat tatcacagtt tattactctg aattggctat ccgcgtgtgt acctctacct
     8221 ggagtttttc ccacggtgga tatttcttct tgcgctgagc gtaagagcta tctgacagaa
     8281 cagttcttct ttgcttcctc gccagttcgc tcgctatgct cggttacacg gctgcggcga
     8341 gcgctagtga taataagtga ctgaggtatg tgctcttctt atctcctttt gtagtgttgc
     8401 tcttatttta aacaactttg cggttttttg atgactttgc gattttgttg ttgctttgca
     8461 gtaaattgca agatttaata aaaaaacgca aagcaatgat taaaggatgt tcagaatgaa
     8521 actcatggaa acacttaacc agtgcataaa cgctggtcat gaaatgacga aggctatcgc
     8581 cattgcacag tttaatgatg acagcccgga agcgaggaaa ataacccggc gctggagaat
     8641 aggtgaagca gcggatttag ttggggtttc ttctcaggct atcagagatg ccgagaaagc
     8701 agggcgacta ccgcacccgg atatggaaat tcgaggacgg gttgagcaac gtgttggtta
     8761 tacaattgaa caaattaatc atatgcgtga tgtgtttggt acgcgattgc gacgtgctga
     8821 agacgtattt ccaccggtga tcggggttgc tgcccataaa ggtggcgttt acaaaacctc
     8881 agtttctgtt catcttgctc aggatctggc tctgaagggg ctacgtgttt tgctcgtgga
     8941 aggtaacgac ccccagggaa cagcctcaat gtatcacgga tgggtaccag atcttcatat
     9001 tcatgcagaa gacactctcc tgcctttcta tcttggggaa aaggacgatg tcacttatgc
     9061 aataaagccc acttgctggc cggggcttga cattattcct tcctgtctgg ctctgcaccg
     9121 tattgaaact gagttaatgg gcaaatttga tgaaggtaaa ctgcccaccg atccacacct
     9181 gatgttccga ctggccattg aaactgttgc tcatgactat gatgtcatag ttattgacag
     9241 cgcgcctaac ctgggtatcg gcacgattaa tgtcgtatgt gctgctgatg tgctgattgt
     9301 tcccacgcct gctgagttgt ttgactacac ctccgcactg cagtttttcg atatgcttcg
     9361 tgatctgctc aagaacgttg atcttaaagg gttcgagcct gatgtacgta ttttgcttac
     9421 caaatacagc aatagtaatg gctctcagtc cccgtggatg gaggagcaaa ttcgggatgc
     9481 ctggggaagc atggttctaa aaaatgttgt acgtgaaacg gatgaagttg gtaaaggtca
     9541 gatccggatg agaactgttt ttgaacaggc cattgatcaa cgctcttcaa ctggtgcctg
     9601 gagaaatgct ctttctattt gggaacctgt ctgcaatgaa attttcgatc gtctgattaa
     9661 accacgctgg gagattagat aatgaagcgt gcgcctgtta ttccaaaaca tacgctcaat
     9721 actcaaccgg ttgaagatac ttcgttatcg acaccagctg ccccgatggt ggattcgtta
     9781 attgcgcgcg taggagtaat ggctcgcggt aatgccatta ctttgcctgt atgtggtcgg
     9841 gatgtgaagt ttactcttga agtgctccgg ggtgatagtg ttgagaagac ctctcgggta
     9901 tggtcaggta atgaacgtga ccaggagctg cttactgagg acgcactgga tgatctcatc
     9961 ccttcttttc tactgactgg tcaacagaca ccggcgttcg gtcgaagagt atctggtgtc
    10021 atagaaattg ccgatgggag tcgccgtcgt aaagctgctg cacttaccga aagtgattat
    10081 cgtgttctgg ttggcgagct ggatgatgag cagatggctg cattatccag attgggtaac
    10141 gattatcgcc caacaagtgc ttatgaacgt ggtcagcgtt atgcaagccg attgcagaat
    10201 gaatttgctg gaaatatttc tgcgctggct gatgcggaaa atatttcacg taagattatt
    10261 acccgctgta tcaacaccgc caaattgcct aaatcagttg ttgctctttt ttctcacccc
    10321 ggtgaactat ctgcccggtc aggtgatgca cttcaaaaag cctttacaga taaagaggaa
    10381 ttacttaagc agcaggcatc taaccttcat gagcagaaaa aagctggggt gatatttgaa
    10441 gctgaagaag ttatcactct tttaacttct gtgcttaaaa cgtcatctgc atcaagaact
    10501 agtttaagct cacgacatca gtttgctcct ggagcgacag tattgtataa gggcgataaa
    10561 atggtgctta acctggacag gtctcgtgtt ccaactgagt gtatagagaa aattgaggcc
    10621 attcttaagg aacttgaaaa gccagcaccc tgatgcgacc acgttttagt ctacgtttat
    10681 ctgtctttac ttaatgtcct ttgttacagg ccagaaagca taactggcct gaatattctc
    10741 tctgggccca ctgttccact tgtatcgtcg gtctgataat cagactggga ccacggtccc
    10801 actcgtatcg tcggtctgat tattagtctg ggaccacggt cccactcgta tcgtcggtct
    10861 gattattagt ctgggaccac ggtcccactc gtatcgtcgg tctgataatc agactgggac
    10921 cacggtccca ctcgtatcgt cggtctgatt attagtctgg gaccatggtc ccactcgtat
    10981 cgtcggtctg attattagtc tgggaccacg gtcccactcg tatcgtcggt ctgattatta
    11041 gtctggaacc acggtcccac tcgtatcgtc ggtctgatta ttagtctggg accacggtcc
    11101 cactcgtatc gtcggtctga ttattagtct gggaccacga tcccactcgt gttgtcggtc
    11161 tgattatcgg tctgggacca cggtcccact tgtattgtcg atcagactat cagcgtgaga
    11221 ctacgattcc atcaatgcct gtcaagggca agtattgaca tgtcgtcgta acctgt
//
