LOCUS       sequence-479267-        3269 bp    DNA     circular SYN 28-JUL-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 28, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_MERS-CoV-nsp10
FEATURES             Location/Qualifiers
     source          1..3269
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     695..711
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     695..711
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     716..735
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        716..734
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    752..851
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1331..1430)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1446..1463)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1446..1462)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1455..1477)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      1526..1612
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1704..1731
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1890..1907)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2061..2649)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2141..2160)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2742..3269,1..264))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gacttctaca gcgcggagaa tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc
       61 gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc
      121 aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag
      181 caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac
      241 ttcggcgatc accgcttccc tcataatgtt taactttgtt ttagggcgac tgccctgctg
      301 cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg
      361 cttggatgcc cgaggcatag actgtacccc aaaaaaacag tcataacaag ccatgaaaac
      421 cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg
      481 catacgctac ttgcattaca gcttacgaac cgaacaggct tatgtccact gggttcgtgc
      541 cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg ggtagcagcg aagtcgaggc
      601 atttctgtcc tggctggtct agaggaggca tgatgatata tttttatctt gtgcaatgta
      661 acatcagaga ttttgagaca cgggccagag ctgccaggaa acagctatga ccatgtaata
      721 cgactcacta taggggatat cagctggatg gcaaataatg attttatttt gactgatagt
      781 gacctgttcg ttgcaacaaa ttgatgagca attatttttt ataatgccaa ctttgtacaa
      841 gaaagctggg cccatgaggc cgcgatcgcg tcttaattaa gttggggcag tgctgcttgc
      901 ctaagcgagt cacaattgca cccatatcca atccaatatt gacagacatt acagggggta
      961 tttgacaaac aaaatcccac agggtcacgg acacactgag cagggatttg gacaaactta
     1021 cccttatatt tacaaacacc agagacatca ggatgttcta tatgcgcacg gcaatagaga
     1081 cacactgaag ctccaccata agtctcttga tcagctgtac tctctggttt aacagatata
     1141 gctatacctg taccagtttt aggagtaagc atcttaacac aatttgtcaa tggggcacct
     1201 cccgcattga cgaaatcgag ataagctttt tgaggatcaa cggtgaagtt aacaagtgac
     1261 aacaccgagg aattagaggc aaactcggtg ttagaaccag cggatcccat ggatcctggc
     1321 cttgacggcc cttctgcttt tttgtacaaa gttggcatta taaaaaagca ttgctcatca
     1381 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg gggcccgagc
     1441 ttaagactgg ccgtcgtttt acaacgtcgt gactgggaaa acatccatgc tagcgttaac
     1501 gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcgg aagactgggc
     1561 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg
     1621 agcggatttg aacgttgtga agcaacggcc cggagggtgg cgggcaggac gcccgccata
     1681 aactgccagg catcaaacta agcagaaggc catcctgacg gatggccttt ttgcgtttct
     1741 acaaactctt cctggctagc ggtacgcgta ttaattgcgt tgcgctcact gcccgctttc
     1801 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     1861 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     1921 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     1981 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     2041 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     2101 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2161 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     2221 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     2281 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     2341 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     2401 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     2461 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     2521 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     2581 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     2641 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgacgcg
     2701 taactcacgt taagggattt tggtcatggg tggctcgagg gttatttgcc gactaccttg
     2761 gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag
     2821 cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg
     2881 gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt
     2941 actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag
     3001 tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca
     3061 ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt
     3121 gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca
     3181 agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac
     3241 ggaatgatgt cgtcgtgcac aacaatggt
//
