LOCUS       sequence-479273-        3074 bp    DNA     circular SYN 03-AUG-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 3, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_SARS-CoV-1-Envelope
FEATURES             Location/Qualifiers
     source          1..3074
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             155..946
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1039..1627
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1528..1547
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1781..1798
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1957..1984)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2076..2162)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2211..2233
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2225..2242
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2226..2242
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2258..2357
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2642..2741)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2758..2777)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2759..2777)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2782..2798)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2782..2798)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca
       61 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
      121 gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga tcgccgaagt
      181 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
      241 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga
      301 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
      361 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
      421 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
      481 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
      541 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
      601 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
      661 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
      721 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
      781 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
      841 ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
      901 tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct cgagccaccc
      961 atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga
     1021 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     1081 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     1141 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     1201 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     1261 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     1321 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     1381 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     1441 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     1501 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     1561 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     1621 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     1681 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     1741 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     1801 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     1861 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatac
     1921 gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc
     1981 ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc
     2041 cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc
     2101 accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt tcgttttatt
     2161 tgatgcctgg cagttcccta ctctcgcgtt aacgctagca tggatgtttt cccagtcacg
     2221 acgttgtaaa acgacggcca gtcttaagct cgggccccaa ataatgattt tattttgact
     2281 gatagtgacc tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt
     2341 gtacaaaaaa gcagaagggc cgtcaaggcc aggatccatg ggatcctact cattcgtttc
     2401 ggaagaaaca ggtacgttaa tagttaatag cgtacttctt tttcttgctt tcgtggtatt
     2461 cttgctagtc acactagcca tccttactgc gcttcgattg tgtgcgtact gctgcaatat
     2521 tgttaacgtg agtttagtaa aaccaacggt ttacgtctac tcgcgtgtta aaaatctgaa
     2581 ctcttctgaa ggagttcctg atcttctggt ccttaattaa gacgcgatcg cggcctcatg
     2641 ggcccagctt tcttgtacaa agttggcatt ataaaaaata attgctcatc aatttgttgc
     2701 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc
     2761 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa
     2821 aatctctgat gttacattgc acaagataaa aatatatcat catgcctcct ctagaccagc
     2881 caggacagaa atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg cacaccgtgg
     2941 aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc
     3001 aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac
     3061 ggcgcagtgg cggt
//
