LOCUS       sequence-479275-        3266 bp    DNA     circular SYN 10-AUG-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 10, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_SARS-CoV-1-nsp10
FEATURES             Location/Qualifiers
     source          1..3266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             129..920
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1013..1601
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1502..1521
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1755..1772
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1931..1958)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2050..2136)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2185..2207
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2199..2216
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2200..2216
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2232..2331
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2808..2907)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2924..2943)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2925..2943)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2948..2964)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2948..2964)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
       61 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
      121 taaacattat gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
      181 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
      241 tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc
      301 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
      361 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
      421 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
      481 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
      541 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
      601 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
      661 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
      721 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
      781 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
      841 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca
      901 ccaaggtagt cggcaaataa ccctcgagcc acccatgacc aaaatccctt aacgtgagtt
      961 acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     1021 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     1081 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     1141 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     1201 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     1261 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     1321 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     1381 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     1441 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     1501 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     1561 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     1621 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     1681 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     1741 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     1801 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     1861 ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag gaagagtttg
     1921 tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat gcctggcagt
     1981 ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc
     2041 tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa
     2101 ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc cctactctcg
     2161 cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg gccagtctta
     2221 agctcgggcc ccaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa
     2281 ttgatgagca atgctttttt ataatgccaa ctttgtacaa aaaagcagaa gggccgtcaa
     2341 ggccaggatc catgggatcc gctggaaatg ctacagaagt acctgccaat tcaactgtgc
     2401 tttccttctg tgcttttgca gtagaccctg ctaaagcata taaggattac ctagcaagtg
     2461 gaggacaacc aatcaccaac tgtgtgaaga tgttgtgtac acacactggt acaggacagg
     2521 caattactgt aacaccagaa gctaacatgg accaagagtc ctttggtggt gcttcatgtt
     2581 gtctgtattg tagatgccac attgaccatc caaatcctaa aggattctgt gacttgaaag
     2641 gtaagtacgt ccaaatacct accacttgtg ctaatgaccc agtgggtttt acacttagaa
     2701 acacagtctg taccgtctgc ggaatgtgga aaggttatgg ctgtagttgt gaccaactcc
     2761 gcgaaccctt gatgcagctt aattaagacg cgatcgcggc ctcatgggcc cagctttctt
     2821 gtacaaagtt ggcattataa aaaataattg ctcatcaatt tgttgcaacg aacaggtcac
     2881 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
     2941 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     3001 cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg acagaaatgc
     3061 ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc
     3121 acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg
     3181 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
     3241 tttcatggct tgttatgact gttttt
//
