LOCUS       sequence-479276-        3887 bp    DNA     circular SYN 10-AUG-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 10, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_SARS-CoV-1-nsp15
FEATURES             Location/Qualifiers
     source          1..3887
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     773..789
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     773..789
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     794..813
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        794..812
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    830..929
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2027..2126)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2142..2159)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2142..2158)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2151..2173)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      2222..2308
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2400..2427
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2586..2603)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2757..3345)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2837..2856)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3438..3887,1..342))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gcgctgccat tctccaaatt gcagttcgcg cttagctgga taacgccacg gaatgatgtc
       61 gtcgtgcaca acaatggtga cttctacagc gcggagaatc tcgctctctc caggggaagc
      121 cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt
      181 caccgtaacc agcaaatcaa tatcactgtg tggcttcagg ccgccatcca ctgcggagcc
      241 gtacaaatgt acggccagca acgtcggttc gagatggcgc tcgatgacgc caactacctc
      301 tgatagttga gtcgatactt cggcgatcac cgcttccctc ataatgttta actttgtttt
      361 agggcgactg ccctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac
      421 ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtaccccaa aaaaacagtc
      481 ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct
      541 ggaccagttg cgtgagcgca tacgctactt gcattacagc ttacgaaccg aacaggctta
      601 tgtccactgg gttcgtgcct tcatccgttt ccacggtgtg cgtcacccgg caaccttggg
      661 tagcagcgaa gtcgaggcat ttctgtcctg gctggtctag aggaggcatg atgatatatt
      721 tttatcttgt gcaatgtaac atcagagatt ttgagacacg ggccagagct gccaggaaac
      781 agctatgacc atgtaatacg actcactata ggggatatca gctggatggc aaataatgat
      841 tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat tattttttat
      901 aatgccaact ttgtacaaga aagctgggcc catgaggccg cgatcgcgtc ttaattaagt
      961 tgtagttttg ggtagaaggt ttcaacatgt ccatccttac accaaagcat gaatgaaatt
     1021 tcagcatagt caattgtaac cttgaccact tttgaaatca ctgacaaatc ttgtgacttt
     1081 attatctcga caaagtcatc aagtaaaaga tcaatcacag aacacacaca ttttgatgaa
     1141 cctgtttgcg catctgttat gaagtaattt ttcactgtgc tgtccatagg gataaaatcc
     1201 tctaatttaa gtggtgaatc ttgtgagcgc ttggctaagc ctatcattaa atgaagaccg
     1261 ccaagttgtc catgactgaa atctccataa acgatgtgtt cgaaggcata gccctcgagc
     1321 ttatatcgct gtatgaattc atccatagcg agctcgagaa agtcagtttc catttgtgat
     1381 ctgggcttaa aatcctctaa gtctctgctc tgagtaaagt aggtttcagg caactgttga
     1441 ataatgccgt ctactttctt aaagtagtta aactgtgttt ttactgattc tccaattaat
     1501 gtgactccat tgacgctagc ttgtgctggt ccctttgaag gtgttagacc tttgactgaa
     1561 ccttctgtta ttaaaacacc attacgggcg tttctaaaaa ggtctacctg tccttccact
     1621 ctaccatcaa acaagacagt aagtgaagaa caagcactct cagtaggttt cttggcaatg
     1681 tcagtcattg tgcagacacc tattgtagat acatgtgctg gggcttctct tttgtagtcc
     1741 cagattacag tattagcagc gatatcaaca cccaaattat tgagtatctt aatctctggc
     1801 actggtttaa tgttacgctt agcccaaagc tcaaatgcaa cattaacagg aagtgttgtc
     1861 ttattttcaa agatctccac atcaatacca tctacctttg tgtaaacagc attattaatg
     1921 atggaaacag gtgcttcgcc ggcgtgtcca tcaaagtgtc ctttattaac aacattataa
     1981 gccacatttt ctaaactgga tcccatggat cctggccttg acggcccttc tgcttttttg
     2041 tacaaagttg gcattataaa aaagcattgc tcatcaattt gttgcaacga acaggtcact
     2101 atcagtcaaa ataaaatcat tatttggggc ccgagcttaa gactggccgt cgttttacaa
     2161 cgtcgtgact gggaaaacat ccatgctagc gttaacgcga gagtagggaa ctgccaggca
     2221 tcaaataaaa cgaaaggctc agtcggaaga ctgggccttt cgttttatct gttgtttgtc
     2281 ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgtgaagca
     2341 acggcccgga gggtggcggg caggacgccc gccataaact gccaggcatc aaactaagca
     2401 gaaggccatc ctgacggatg gcctttttgc gtttctacaa actcttcctg gctagcggta
     2461 cgcgtattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     2521 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     2581 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     2641 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     2701 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     2761 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     2821 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     2881 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     2941 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     3001 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     3061 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     3121 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     3181 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     3241 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     3301 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     3361 ttttctacgg ggtctgacgc tcagtggaac gacgcgtaac tcacgttaag ggattttggt
     3421 catgggtggc tcgagggtta tttgccgact accttggtga tctcgccttt cacgtagtgg
     3481 acaaattctt ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg tccaagataa
     3541 gcctgtctag cttcaagtat gacgggctga tactgggccg gcaggcgctc cattgcccag
     3601 tcggcagcga catccttcgg cgcgattttg ccggttactg cgctgtacca aatgcgggac
     3661 aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt
     3721 aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc
     3781 gccgctggac ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa gatagccaga
     3841 tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa tgtcatt
//
