LOCUS       sequence-479282-        3098 bp    DNA     circular SYN 25-JUL-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 25, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_SARS-CoV-1-ORF8b
FEATURES             Location/Qualifiers
     source          1..3098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             297..1088
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1181..1769
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1670..1689
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1923..1940
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2099..2126)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2218..2304)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2353..2375
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2367..2384
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2368..2384
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2400..2499
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2808..2907)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2924..2943)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2925..2943)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2948..2964)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2948..2964)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 caccgtggaa acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc
       61 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
      121 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt
      181 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
      241 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacattatga
      301 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
      361 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
      421 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
      481 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
      541 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
      601 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
      661 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
      721 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
      781 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
      841 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
      901 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
      961 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
     1021 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
     1081 gcaaataacc ctcgagccac ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc
     1141 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1201 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1261 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1321 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     1381 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     1441 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     1501 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     1561 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     1621 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     1681 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     1741 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     1801 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     1861 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     1921 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     1981 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     2041 gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta gaaacgcaaa
     2101 aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg
     2161 tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt
     2221 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttc
     2281 cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg ttaacgctag
     2341 catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag ctcgggcccc
     2401 aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat
     2461 gcttttttat aatgccaact ttgtacaaaa aagcagaagg gccgtcaagg ccaggatcca
     2521 tgggatcctg cttgaagatc cttgtaaggt acaacactag gggtaatact tatagcactg
     2581 cttggctttg tgctctagga aaggttttac cttttcatag atggcacact atggttcaaa
     2641 catgcacacc taatgttact atcaactgtc aagatccagc tggtggtgcg cttatagcta
     2701 ggtgttggta ccttcatgaa ggtcaccaaa ctgctgcatt tagagacgta cttgttgttt
     2761 taaataaacg aacaaatctt aattaagacg cgatcgcggc ctcatgggcc cagctttctt
     2821 gtacaaagtt ggcattataa aaaataattg ctcatcaatt tgttgcaacg aacaggtcac
     2881 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
     2941 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     3001 cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg acagaaatgc
     3061 ctcgacttcg ctgctaccca aggttgccgg gtgacgca
//
