LOCUS       sequence-479283-        3098 bp    DNA     circular SYN 24-JUL-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 24, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_SARS-CoV-2-nsp7
FEATURES             Location/Qualifiers
     source          1..3098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(9..26)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(180..768)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(260..279)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(861..1652)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     2083..2099
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2083..2099
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2104..2123
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2104..2122
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2140..2239
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2548..2647)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2663..2680)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2663..2679)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2672..2694)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      2743..2829
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2921..2948
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
       61 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
      121 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
      181 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
      241 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
      301 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
      361 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
      421 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
      481 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
      541 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
      601 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
      661 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
      721 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
      781 atcttttcta cggggtctga cgctcagtgg aacgacgcgt aactcacgtt aagggatttt
      841 ggtcatgggt ggctcgaggg ttatttgccg actaccttgg tgatctcgcc tttcacgtag
      901 tggacaaatt cttccaactg atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga
      961 taagcctgtc tagcttcaag tatgacgggc tgatactggg ccggcaggcg ctccattgcc
     1021 cagtcggcag cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg
     1081 gacaacgtaa gcactacatt tcgctcatcg ccagcccagt cgggcggcga gttccatagc
     1141 gttaaggttt catttagcgc ctcaaataga tcctgttcag gaaccggatc aaagagttcc
     1201 tccgccgctg gacctaccaa ggcaacgcta tgttctcttg cttttgtcag caagatagcc
     1261 agatcaatgt cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt gcgctgccat
     1321 tctccaaatt gcagttcgcg cttagctgga taacgccacg gaatgatgtc gtcgtgcaca
     1381 acaatggtga cttctacagc gcggagaatc tcgctctctc caggggaagc cgaagtttcc
     1441 aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc
     1501 agcaaatcaa tatcactgtg tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt
     1561 acggccagca acgtcggttc gagatggcgc tcgatgacgc caactacctc tgatagttga
     1621 gtcgatactt cggcgatcac cgcttccctc ataatgttta actttgtttt agggcgactg
     1681 ccctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc
     1741 gcttgctgct tggatgcccg aggcatagac tgtaccccaa aaaaacagtc ataacaagcc
     1801 atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg
     1861 cgtgagcgca tacgctactt gcattacagc ttacgaaccg aacaggctta tgtccactgg
     1921 gttcgtgcct tcatccgttt ccacggtgtg cgtcacccgg caaccttggg tagcagcgaa
     1981 gtcgaggcat ttctgtcctg gctggtctag aggaggcatg atgatatatt tttatcttgt
     2041 gcaatgtaac atcagagatt ttgagacacg ggccagagct gccaggaaac agctatgacc
     2101 atgtaatacg actcactata ggggatatca gctggatggc aaataatgat tttattttga
     2161 ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat tattttttat aatgccaact
     2221 ttgtacaaga aagctgggcc catgaggccg cgatcgcgtc ttaattaagt tgtaaggttg
     2281 ccctgttgtc cagcatttct tcacaaagct tgtttatgtc tacagcaccc tgcatggaaa
     2341 gcaaaacaga aagtagtgaa accatttttt caaaggcttc agtagtatct ttagctaaga
     2401 gaatgtcatt gtgtaactgg acacattgag cccacaattt agatgatgat tctactctga
     2461 gttgttgcaa aactgagagt aagactactg atgtgcactt tacatctgac attttagagg
     2521 atcccatgga tcctggcctt gacggccctt ctgctttttt gtacaaagtt ggcattataa
     2581 aaaagcattg ctcatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
     2641 ttatttgggg cccgagctta agactggccg tcgttttaca acgtcgtgac tgggaaaaca
     2701 tccatgctag cgttaacgcg agagtaggga actgccaggc atcaaataaa acgaaaggct
     2761 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
     2821 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
     2881 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
     2941 ggcctttttg cgtttctaca aactcttcct ggctagcggt acgcgtatta attgcgttgc
     3001 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     3061 aacgcgcggg gagaggcggt ttgcgtattg ggcgctct
//
