LOCUS       sequence-479287-        2912 bp    DNA     circular SYN 26-JUL-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 26, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_SARS-CoV-2-ORF3b
FEATURES             Location/Qualifiers
     source          1..2912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(130..229)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(246..265)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(247..265)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(270..286)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(270..286)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             717..1508
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1601..2189
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2090..2109
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2343..2360
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2519..2546)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2638..2724)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2773..2795
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2787..2804
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2788..2804
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    join(2820..2912,1..7)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gcagaagggc cgtcaaggcc aggatccatg ggatccatgc caactatttt ctttgctggc
       61 atactaattg ttacgactat tgtatacctt acaatagtgc ttaattaaga cgcgatcgcg
      121 gcctcatggg cccagctttc ttgtacaaag ttggcattat aaaaaataat tgctcatcaa
      181 tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg
      241 atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg
      301 tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca tgcctcctct
      361 agaccagcca ggacagaaat gcctcgactt cgctgctacc caaggttgcc gggtgacgca
      421 caccgtggaa acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc
      481 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
      541 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt
      601 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
      661 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacattatga
      721 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
      781 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
      841 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
      901 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
      961 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
     1021 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
     1081 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
     1141 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
     1201 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
     1261 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
     1321 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
     1381 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
     1441 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
     1501 gcaaataacc ctcgagccac ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc
     1561 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1621 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1681 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1741 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     1801 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     1861 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     1921 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     1981 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2041 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2101 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2161 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     2221 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     2281 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     2341 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     2401 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     2461 gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta gaaacgcaaa
     2521 aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg
     2581 tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt
     2641 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttc
     2701 cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg ttaacgctag
     2761 catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag ctcgggcccc
     2821 aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat
     2881 gcttttttat aatgccaact ttgtacaaaa aa
//
