LOCUS       sequence-479289-        3029 bp    DNA     circular SYN 04-AUG-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_SARS-CoV-2-ORF6
FEATURES             Location/Qualifiers
     source          1..3029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             145..936
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1029..1617
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1518..1537
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1771..1788
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1947..1974)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2066..2152)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2201..2223
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2215..2232
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2216..2232
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2248..2347
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2587..2686)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2703..2722)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2704..2722)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2727..2743)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2727..2743)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt
       61 tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt
      121 cgccctaaaa caaagttaaa cattatgagg gaagcggtga tcgccgaagt atcgactcaa
      181 ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat
      241 ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt
      301 acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa
      361 acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg
      421 cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg
      481 cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct
      541 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa
      601 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta
      661 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc
      721 atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca
      781 atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt
      841 ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac
      901 gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct cgagccaccc atgaccaaaa
      961 tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa
     1021 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     1081 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     1141 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     1201 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     1261 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     1321 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     1381 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     1441 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     1501 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     1561 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     1621 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     1681 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     1741 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     1801 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     1861 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct
     1921 agccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag
     1981 tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca
     2041 caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac
     2101 aacagataaa acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg
     2161 cagttcccta ctctcgcgtt aacgctagca tggatgtttt cccagtcacg acgttgtaaa
     2221 acgacggcca gtcttaagct cgggccccaa ataatgattt tattttgact gatagtgacc
     2281 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa
     2341 gcagaagggc cgtcaaggcc aggatccatg ggatcctttc atctcgttga ctttcaggtt
     2401 actatagcag agatattact aattattatg aggactttta aagtttccat ttggaatctt
     2461 gattacatca taaacctcat aattaaaaat ttatctaagt cactaactga gaataaatat
     2521 tctcaattag atgaagagca accaatggag attgatctta attaagacgc gatcgcggcc
     2581 tcatgggccc agctttcttg tacaaagttg gcattataaa aaataattgc tcatcaattt
     2641 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata
     2701 tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt
     2761 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga
     2821 ccagccagga cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac
     2881 cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
     2941 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     3001 gtaacggcgc agtggcggtt ttcatggct
//
