LOCUS       sequence-479291-        4763 bp    DNA     circular SYN 10-AUG-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 10, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_SARS-CoV-2-nsp2
FEATURES             Location/Qualifiers
     source          1..4763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             357..1148
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1241..1829
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1730..1749
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1983..2000
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2159..2186)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2278..2364)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2413..2435
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2427..2444
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2428..2444
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2460..2559
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4533..4632)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4649..4668)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4650..4668)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4673..4689)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4673..4689)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agaccagcca ggacagaaat gcctcgactt cgctgctacc caaggttgcc gggtgacgca
       61 caccgtggaa acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc
      121 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
      181 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt
      241 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
      301 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacattatga
      361 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
      421 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
      481 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
      541 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
      601 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
      661 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
      721 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
      781 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
      841 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
      901 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
      961 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
     1021 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
     1081 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
     1141 gcaaataacc ctcgagccac ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc
     1201 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1261 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1321 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1381 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     1441 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     1501 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     1561 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     1621 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     1681 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     1741 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     1801 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     1861 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     1921 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     1981 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     2041 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     2101 gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta gaaacgcaaa
     2161 aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg
     2221 tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt
     2281 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttc
     2341 cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg ttaacgctag
     2401 catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag ctcgggcccc
     2461 aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat
     2521 gcttttttat aatgccaact ttgtacaaaa aagcagaagg gccgtcaagg ccaggatcca
     2581 tgggatccgc atacactcgc tatgtcgata acaacttctg tggccctgat ggctaccctc
     2641 ttgagtgcat taaagacctt ctagcacgtg ctggtaaagc ttcatgcact ttgtccgaac
     2701 aactggactt tattgacact aagaggggtg tatactgctg ccgtgaacat gagcatgaaa
     2761 ttgcttggta cacggaacgt tctgaaaaga gctatgaatt gcagacacct tttgaaatta
     2821 aattggcaaa gaaatttgac accttcaatg gggaatgtcc aaattttgta tttcccttaa
     2881 attccataat caagactatt caaccaaggg ttgaaaagaa aaagcttgat ggctttatgg
     2941 gtagaattcg atctgtctat ccagttgcgt caccaaatga atgcaaccaa atgtgccttt
     3001 caactctcat gaagtgtgat cattgtggtg aaacttcatg gcagacgggc gattttgtta
     3061 aagccacttg cgaattttgt ggcactgaga atttgactaa agaaggtgcc actacttgtg
     3121 gttacttacc ccaaaatgct gttgttaaaa tttattgtcc agcatgtcac aattcagaag
     3181 taggacctga gcatagtctt gccgaatacc ataatgaatc tggcttgaaa accattcttc
     3241 gtaagggtgg tcgcactatt gcctttggag gctgtgtgtt ctcttatgtt ggttgccata
     3301 acaagtgtgc ctattgggtt ccacgtgcta gcgctaacat aggttgtaac catacaggtg
     3361 ttgttggaga aggttccgaa ggtcttaatg acaaccttct tgaaatactc caaaaagaga
     3421 aagtcaacat caatattgtt ggtgacttta aacttaatga agagatcgcc attattttgg
     3481 catctttttc tgcttccaca agtgcttttg tggaaactgt gaaaggtttg gattataaag
     3541 cattcaaaca aattgttgaa tcctgtggta attttaaagt tacaaaagga aaagctaaaa
     3601 aaggtgcctg gaatattggt gaacagaaat caatactgag tcctctttat gcatttgcat
     3661 cagaggctgc tcgtgttgta cgatcaattt tctcccgcac tcttgaaact gctcaaaatt
     3721 ctgtgcgtgt tttacagaag gccgctataa caatactaga tggaatttca cagtattcac
     3781 tgagactcat tgatgctatg atgttcacat ctgatttggc tactaacaat ctagttgtaa
     3841 tggcctacat tacaggtggt gttgttcagt tgacttcgca gtggctaact aacatctttg
     3901 gcactgttta tgaaaaactc aaacccgtcc ttgattggct tgaagagaag tttaaggaag
     3961 gtgtagagtt tcttagagac ggttgggaaa ttgttaaatt tatctcaacc tgtgcttgtg
     4021 aaattgtcgg tggacaaatt gtcacctgtg caaaggaaat taaggagagt gttcagacat
     4081 tctttaagct tgtaaataaa tttttggctt tgtgtgctga ctctatcatt attggtggag
     4141 ctaaacttaa agccttgaat ttaggtgaaa catttgtcac gcactcaaag ggattgtaca
     4201 gaaagtgtgt taaatccaga gaagaaactg gcctactcat gcctctaaaa gccccaaaag
     4261 aaattatctt cttagaggga gaaacacttc ccacagaagt gttaacagag gaagttgtct
     4321 tgaaaactgg tgatttacaa ccattagaac aacctactag tgaagctgtt gaagctccat
     4381 tggttggtac accagtttgt attaacgggc ttatgttgct cgaaatcaaa gacacagaaa
     4441 agtactgtgc ccttgcacct aatatgatgg taacaaacaa taccttcaca ctcaaaggcg
     4501 gtcttaatta agacgcgatc gcggcctcat gggcccagct ttcttgtaca aagttggcat
     4561 tataaaaaat aattgctcat caatttgttg caacgaacag gtcactatca gtcaaaataa
     4621 aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc
     4681 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
     4741 aaatatatca tcatgcctcc tct
//
