LOCUS       sequence-479292-        5645 bp    DNA     circular SYN 23-JUL-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 5645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 23, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_SARS-CoV-2-nsp12
FEATURES             Location/Qualifiers
     source          1..5645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             125..916
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1009..1597
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1498..1517
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1751..1768
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1927..1954)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2046..2132)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2181..2203
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2195..2212
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2196..2212
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2228..2327
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(5183..5282)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5299..5318)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5300..5318)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5323..5339)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5323..5339)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
       61 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa
      121 cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
      181 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
      241 tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga
      301 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
      361 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
      421 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
      481 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
      541 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
      601 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
      661 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
      721 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
      781 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
      841 ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa
      901 ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg tgagttacgc
      961 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1021 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1081 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1141 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     1201 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     1261 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1321 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     1381 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     1441 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     1501 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     1561 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     1621 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     1681 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     1741 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     1801 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     1861 agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga
     1921 aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat
     1981 ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc
     2041 ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc
     2101 cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt
     2161 aacgctagca tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct
     2221 cgggccccaa ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga
     2281 tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa gcagaagggc cgtcaaggcc
     2341 aggatccatg ggatcctcag ctgatgcaca atcgttttta aaccgggttt gcggtgtaag
     2401 tgcagcccgt cttacaccgt gcggcacagg cactagtact gatgtcgtat acagggcttt
     2461 tgacatctac aatgataaag tagctggttt tgctaaattc ctaaaaacta attgttgtcg
     2521 cttccaagaa aaggacgaag atgacaattt aattgattct tactttgtag ttaagagaca
     2581 cactttctct aactaccaac atgaagaaac aatttataat ttacttaagg attgtccagc
     2641 tgttgctaaa catgacttct ttaagtttag aatagacggt gacatggtac cacatatatc
     2701 acgtcaacgt cttactaaat acacaatggc agacctcgtc tatgctttaa ggcattttga
     2761 tgaaggtaat tgtgacacat taaaagaaat acttgtcaca tacaattgtt gtgatgatga
     2821 ttatttcaat aaaaaggact ggtatgattt tgtagaaaac ccagatatat tacgcgtata
     2881 cgccaactta ggtgaacgtg tacgccaagc tttgttaaaa acagtacaat tctgtgatgc
     2941 catgcgaaat gctggtattg ttggtgtact gacattagat aatcaagatc tcaatggtaa
     3001 ctggtatgat ttcggtgatt tcatacaaac cacgccaggt agtggagttc ctgttgtaga
     3061 ttcttattat tcattgttaa tgcctatatt aaccttgacc agggctttaa ctgcagagtc
     3121 acatgttgac actgacttaa caaagcctta cattaagtgg gatttgttaa aatatgactt
     3181 cacggaagag aggttaaaac tctttgaccg ttattttaaa tattgggatc agacatacca
     3241 cccaaattgt gttaactgtt tggatgacag atgcattctg cattgtgcaa actttaatgt
     3301 tttattctct acagtgttcc cacctacaag ttttggacca ctagtgagaa aaatatttgt
     3361 tgatggtgtt ccatttgtag tttcaactgg ataccacttc agagagctag gtgttgtaca
     3421 taatcaggat gtaaacttac atagctctag acttagtttt aaggaattac ttgtgtatgc
     3481 tgctgaccct gctatgcacg ctgcttctgg taatctatta ctagataaac gcactacgtg
     3541 cttttcagta gctgcactta ctaacaatgt tgcttttcaa actgtcaaac ccggtaattt
     3601 taacaaagac ttctatgact ttgctgtgtc taagggtttc tttaaggaag gaagttctgt
     3661 tgaattaaaa cacttcttct ttgctcagga tggtaatgct gctatcagcg attatgacta
     3721 ctatcgttat aatctaccaa caatgtgtga tatcagacaa ctactatttg tagttgaagt
     3781 tgttgataag tactttgatt gttacgatgg tggctgtatt aatgctaacc aagtcatcgt
     3841 caacaaccta gacaaatcag ctggttttcc atttaataaa tggggtaagg ctagacttta
     3901 ttatgattca atgagttatg aggatcaaga tgcacttttc gcatatacaa aacgtaatgt
     3961 catccctact ataactcaaa tgaatcttaa gtatgccatt agtgcaaaga atagagctcg
     4021 caccgtagct ggtgtctcta tctgtagtac tatgaccaat agacagtttc atcaaaaatt
     4081 attgaaatca atagccgcca ctagaggagc tactgtagta attggaacaa gcaaattcta
     4141 tggtggttgg cacaacatgt taaaaactgt ttatagtgat gtagaaaacc ctcaccttat
     4201 gggttgggat tatcctaaat gtgatagagc catgcctaac atgcttagaa ttatggcctc
     4261 acttgttctt gctcgcaaac atacaacgtg ttgtagcttg tcacaccgtt tctatagatt
     4321 agctaatgag tgtgctcaag tattgagtga aatggtcatg tgtggcggtt cactatatgt
     4381 taaaccaggt ggaacctcat caggagatgc cacaactgct tatgctaata gtgtttttaa
     4441 catttgtcaa gctgtcacgg ccaatgttaa tgcactttta tctactgatg gtaacaaaat
     4501 tgccgataag tatgtccgca atttacaaca cagactttat gagtgtctct atagaaatag
     4561 agatgttgac acagactttg tgaatgagtt ttacgcatat ttgcgtaaac atttctcaat
     4621 gatgatactc tctgacgatg ctgttgtgtg tttcaatagc acttatgcat ctcaaggtct
     4681 agtggctagc ataaagaact ttaagtcagt tctttattat caaaacaatg tttttatgtc
     4741 tgaagcaaaa tgttggactg agactgacct tactaaagga cctcatgaat tttgctctca
     4801 acatacaatg ctagttaaac agggtgatga ttatgtgtac cttccttacc cagatccatc
     4861 aagaatccta ggggccggct gttttgtaga tgatatcgta aaaacagatg gtacacttat
     4921 gattgaacgg ttcgtgtctt tagctataga tgcttaccca cttactaaac atcctaatca
     4981 ggagtatgct gatgtctttc atttgtactt acaatacata agaaagctac atgatgagtt
     5041 aacaggacac atgttagaca tgtattctgt tatgcttact aatgataaca cttcaaggta
     5101 ttgggaacct gagttttatg aggctatgta cacaccgcat acagtcttac agcttaatta
     5161 agacgcgatc gcggcctcat gggcccagct ttcttgtaca aagttggcat tataaaaaat
     5221 aattgctcat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt
     5281 tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg
     5341 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
     5401 tcatgcctcc tctagaccag ccaggacaga aatgcctcga cttcgctgct acccaaggtt
     5461 gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt
     5521 tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg
     5581 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
     5641 tttgg
//
