LOCUS       sequence-479293-        3179 bp    DNA     circular SYN 26-JUL-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 26, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_HCoV-HKU1-nsp9
FEATURES             Location/Qualifiers
     source          1..3179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     132..151
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     385..402
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(561..588)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(680..766)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     815..837
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     829..846
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     830..846
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    862..961
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1351..1450)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1467..1486)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1468..1486)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1491..1507)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1491..1507)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1938..2729
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(2822..3179,1..231)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
       61 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
      121 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
      181 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
      241 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
      301 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
      361 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
      421 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
      481 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag
      541 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat
      601 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt
      661 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
      721 taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc
      781 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg
      841 gccagtctta agctcgggcc ccaaataatg attttatttt gactgatagt gacctgttcg
      901 ttgcaacaaa ttgatgagca atgctttttt ataatgccaa ctttgtacaa aaaagcagaa
      961 gggccgtcaa ggccaggatc catgggatcc aataatgagt tgatgcctca taaattaaaa
     1021 atacaagttg ttaatagtgg ttctgatatg aattgtaata ttcctactca atgttattat
     1081 aataatggta gtagtggtag aatagtttat gctgttctta gtgatgttga tggtcttaag
     1141 tatactaaga taatgaaaga tgatggaaat tgtgttgttt tagagcttga tcctccttgt
     1201 aaattttcta tacaagatgt taagggactt aaaattaagt atctttattt tattaaagga
     1261 tgtaacactt tagctagagg gtgggttgtt ggtactttat cttcaacaat tagattgcag
     1321 cttaattaag acgcgatcgc ggcctcatgg gcccagcttt cttgtacaaa gttggcatta
     1381 taaaaaataa ttgctcatca atttgttgca acgaacaggt cactatcagt caaaataaaa
     1441 tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg
     1501 tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa
     1561 atatatcatc atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac
     1621 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat
     1681 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
     1741 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     1801 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     1861 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     1921 aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag
     1981 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     2041 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
     2101 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     2161 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     2221 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     2281 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     2341 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     2401 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     2461 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     2521 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     2581 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     2641 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
     2701 gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta
     2761 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2821 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     2881 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     2941 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     3001 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3061 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3121 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacg
//
