LOCUS       sequence-479297-        4610 bp    DNA     circular SYN 25-JUL-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 25, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_HCoV-HKU1-nsp2
FEATURES             Location/Qualifiers
     source          1..4610
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     133..152
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     386..403
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(562..589)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(681..767)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     816..838
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     830..847
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     831..847
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    863..962
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2783..2882)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2899..2918)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2900..2918)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2923..2939)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2923..2939)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3370..4161
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(4254..4610,1..232)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
       61 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      121 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      181 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      241 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      301 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
      361 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
      421 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
      481 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta ccgctagcca
      541 ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttagtttga
      601 tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg
      661 ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag
      721 ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg cctggcagtt
      781 ccctactctc gcgttaacgc tagcatggat gttttcccag tcacgacgtt gtaaaacgac
      841 ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag tgacctgttc
      901 gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca aaaaagcaga
      961 agggccgtca aggccaggat ccatgggatc cattaaaccc gtactttttg tagaccagta
     1021 tggttgtgac tattctggta aattagcaga ttgtcttcaa gcttatggtc attattcttt
     1081 gcaagatatg agacaaaagc agtctgtatg gcttgccaat tgtgactttg atattgtagt
     1141 ggcttggcat gtagttcgtg attcacgatt tgttatgcgc ctgcagacta tagctactat
     1201 ttgtggtatt aaatatgttg cacaacctac agaagatgta gtagatggag atgtagttat
     1261 acgtgaacct gtacatttat tatctgctga tgcaatagtt ttaaagcttc ctagtttgat
     1321 gaaagttatg actcatatgg atgatttttc tattaaatct atatataatg ttgatttgtg
     1381 tgattgtggt tttgttatgc agtatggtta tgtagattgt tttaatgata attgtgattt
     1441 ttatggttgg gtttcaggta atatgatgga tggtttttct tgtccattgt gttgtacagt
     1501 ttatgactct agcgaagtta aagcccaatc atctggtgtt attcctgaaa atcctgtgtt
     1561 atttactaat agtactgata ctgttaacca tgattctttt aatttgtatg gttattctgt
     1621 cacaccattt ggttcttgta tatattggtc gccgcgtcct ggattgtgga ttcctataat
     1681 taaatcttca gtcaagtctt atgatgattt ggtttattca ggtgtagtag gttgtaaatc
     1741 tattgttaaa gaaactgctc ttattactca tgcactttac ttagattatg ttcaatgtaa
     1801 gtgtggtaat cttgaacaaa atcatattct tggcgttaat aattcttggt gtaggcaact
     1861 gttgcttaat agaggtgatt ataatatgct tctaaaaaat attgacttgt ttgttaagcg
     1921 tcgtgctgat tttgcttgca agtttgcagt ttgtggagat ggttttgtac cttttttact
     1981 agatggttta attccccgta gttattatct aattcagagt ggtattttct ttacatcttt
     2041 gatgtctcaa ttttcacaag aagtttctga tatgtgttta aaaatgtgta ttttgtttat
     2101 ggacagagtt tcagttgcta cattttatat agagcattat gttaataggt tggttactca
     2161 atttaagtta ttgggtacta cacttgttaa taaaatggtt aattggttta ataccatgtt
     2221 agatgctagt gcacctgcta caggctggct tctttaccaa ttattgaatg gtctttttgt
     2281 agtatctcaa gccaacttta attttgttgc tttaatacct gattatgcta aaattttagt
     2341 taataaattt tacacttttt ttaagttatt attagagtgt gttacagttg atgttttaaa
     2401 agatatgcct gttcttaaaa ctattaatgg tttagtttgt attgtaggca ataagtttta
     2461 taacgttagt acagggttaa ttcctggttt tgttttacca tgtaatgcac aggaacaaca
     2521 aatttatttt tttgaaggcg ttgcagaatc tgttatagta gaagatgatg ttattgagaa
     2581 tgtcaaatct tctttatcat cttatgagta ttgtcaacca cctaaatctg tagaaaaaat
     2641 ttgtattata gataatatgt acatgggtaa gtgtggtgat aaatttttcc ctattgtcat
     2701 gaatgataaa aatatttgtc ttttagatca ggcttggcgt tttccatgtg cacttaatta
     2761 agacgcgatc gcggcctcat gggcccagct ttcttgtaca aagttggcat tataaaaaat
     2821 aattgctcat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt
     2881 tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg
     2941 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
     3001 tcatgcctcc tctagaccag ccaggacaga aatgcctcga cttcgctgct acccaaggtt
     3061 gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt
     3121 tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg
     3181 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
     3241 tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga
     3301 tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
     3361 ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt
     3421 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca
     3481 gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg
     3541 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct
     3601 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt
     3661 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt
     3721 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa
     3781 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt
     3841 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc
     3901 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca
     3961 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg
     4021 gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat
     4081 cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc
     4141 accaaggtag tcggcaaata accctcgagc cacccatgac caaaatccct taacgtgagt
     4201 tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     4261 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     4321 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     4381 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     4441 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     4501 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     4561 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
//
