LOCUS       sequence-479305-        3893 bp    DNA     circular SYN 06-AUG-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_HCoV-229E-nsp15
FEATURES             Location/Qualifiers
     source          1..3893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     89..108
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     342..359
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(518..545)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(637..723)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     772..794
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     786..803
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     787..803
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    819..918
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2022..2121)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2138..2157)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2139..2157)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2162..2178)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2162..2178)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2609..3400
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3493..3893,1..188)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
       61 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      121 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      181 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      241 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      301 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      361 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
      421 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata
      481 cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc
      541 cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg
      601 ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt
      661 caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat
      721 ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac
      781 gacgttgtaa aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac
      841 tgatagtgac ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt
      901 tgtacaaaaa agcagaaggg ccgtcaaggc caggatccat gggatccggt ttagagaaca
      961 ttgcttttaa cgttgttaat aaaggttcat ttgttggtgc tgatggtgaa ttaccagtag
     1021 ccattagtgg tgataaagtg ttcgtacgtg atggtaacac tgataattta gtctttgtta
     1081 acaaaacatc actgcctaca aacatagcat ttgaactttt tgctaagagg aaggttggtt
     1141 taacaccacc tctcagtatt ctcaaaaacc ttggtgttgt cgccacatat aagtttgtct
     1201 tgtgggatta tgaagctgag cgtcccttga caagctttac taagtctgtt tgtggttata
     1261 cagactttgc agaggatgtt tgtacttgtt acgataatag tatacaaggt tcatacgaac
     1321 gttttactct gtcaactaat gctgtgttat tctctgctac tgctgtgaaa acaggtggta
     1381 agagtttgcc ggctattaaa ttgaattttg gaatgcttaa tggtaatgca attgctactg
     1441 tcaaatcaga agatggtaac ataaaaaata ttaactggtt tgtttacgta cgcaaagatg
     1501 gcaaacctgt tgatcattat gatggttttt atacccaagg tcgtaattta caagactttt
     1561 tgcctcgcag cacaatggaa gaagactttt tgaacatgga tataggcgtg tttattcaaa
     1621 agtatggtct agaggatttc aacttcgagc acgttgtgta tggtgatgtt tcaaaaacta
     1681 ctctaggcgg tttacacttg ttgatttcac aagtacgtct gagtaaaatg ggcatcttaa
     1741 aggcagagga gtttgtggca gcatctgaca taacactcaa atgttgtact gtgacttatc
     1801 ttaatgatcc tagttctaag actgtttgta cttacatgga tttgttgttg gatgattttg
     1861 tttctgtatt gaagtctttg gatttgactg ttgtatccaa ggttcatgag gtcataattg
     1921 acaacaaacc atggagatgg atgctatggt gtaaagataa tgccgttgct acattctatc
     1981 ctcagttgca gcttaattaa gacgcgatcg cggcctcatg ggcccagctt tcttgtacaa
     2041 agttggcatt ataaaaaata attgctcatc aatttgttgc aacgaacagg tcactatcag
     2101 tcaaaataaa atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat
     2161 ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc
     2221 acaagataaa aatatatcat catgcctcct ctagaccagc caggacagaa atgcctcgac
     2281 ttcgctgcta cccaaggttg ccgggtgacg cacaccgtgg aaacggatga aggcacgaac
     2341 ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg
     2401 caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat
     2461 ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc
     2521 gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg
     2581 cagtcgccct aaaacaaagt taaacattat gagggaagcg gtgatcgccg aagtatcgac
     2641 tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt
     2701 acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct
     2761 ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt
     2821 ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt
     2881 tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga
     2941 atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct
     3001 ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga
     3061 ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac
     3121 gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc
     3181 ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg
     3241 ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta
     3301 tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca
     3361 ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa ccctcgagcc acccatgacc
     3421 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
     3481 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3541 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3601 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     3661 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3721 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3781 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3841 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaa
//
