LOCUS       sequence-479311-        3521 bp    DNA     circular SYN 01-AUG-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 1, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_HCoV-NL63-Protein3
FEATURES             Location/Qualifiers
     source          1..3521
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(79..96)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(250..838)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(330..349)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(931..1722)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     2153..2169
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2153..2169
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2174..2193
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2174..2192
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2210..2309
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3041..3140)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3156..3173)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3156..3172)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3165..3187)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      3236..3322
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3414..3441
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
       61 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
      121 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
      181 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
      241 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
      301 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
      361 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
      421 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
      481 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
      541 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
      601 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
      661 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
      721 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
      781 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
      841 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgacgcgt aactcacgtt
      901 aagggatttt ggtcatgggt ggctcgaggg ttatttgccg actaccttgg tgatctcgcc
      961 tttcacgtag tggacaaatt cttccaactg atctgcgcgc gaggccaagc gatcttcttc
     1021 ttgtccaaga taagcctgtc tagcttcaag tatgacgggc tgatactggg ccggcaggcg
     1081 ctccattgcc cagtcggcag cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta
     1141 ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg ccagcccagt cgggcggcga
     1201 gttccatagc gttaaggttt catttagcgc ctcaaataga tcctgttcag gaaccggatc
     1261 aaagagttcc tccgccgctg gacctaccaa ggcaacgcta tgttctcttg cttttgtcag
     1321 caagatagcc agatcaatgt cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt
     1381 gcgctgccat tctccaaatt gcagttcgcg cttagctgga taacgccacg gaatgatgtc
     1441 gtcgtgcaca acaatggtga cttctacagc gcggagaatc tcgctctctc caggggaagc
     1501 cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt
     1561 caccgtaacc agcaaatcaa tatcactgtg tggcttcagg ccgccatcca ctgcggagcc
     1621 gtacaaatgt acggccagca acgtcggttc gagatggcgc tcgatgacgc caactacctc
     1681 tgatagttga gtcgatactt cggcgatcac cgcttccctc ataatgttta actttgtttt
     1741 agggcgactg ccctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac
     1801 ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtaccccaa aaaaacagtc
     1861 ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct
     1921 ggaccagttg cgtgagcgca tacgctactt gcattacagc ttacgaaccg aacaggctta
     1981 tgtccactgg gttcgtgcct tcatccgttt ccacggtgtg cgtcacccgg caaccttggg
     2041 tagcagcgaa gtcgaggcat ttctgtcctg gctggtctag aggaggcatg atgatatatt
     2101 tttatcttgt gcaatgtaac atcagagatt ttgagacacg ggccagagct gccaggaaac
     2161 agctatgacc atgtaatacg actcactata ggggatatca gctggatggc aaataatgat
     2221 tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat tattttttat
     2281 aatgccaact ttgtacaaga aagctgggcc catgaggccg cgatcgcgtc ttaattaaga
     2341 ttaatcgaag gaacatcttc gtatagttga gaagagtaga ctataccaac aacaggctct
     2401 tcggcaaaaa tgtaaatgac agcaccatta tacaagtcaa ccttacgcat aagttgtagg
     2461 ttcttttcac aagaacctct aatagcaaca taaaggtcat caaaagaaac aaaagtaata
     2521 gtttcaccac ctaaaacgac atagtggtca ccaccataaa tagcagcaaa acgattttca
     2581 taaaatgatt gttcaagata ccaacacttg cctgaaacga agcatagttt agtaacattg
     2641 aacaaaacaa atgaaaaatt tttatagaga taggcataat agccaacatg tatgaatcga
     2701 aacaacagcg tagaaaaaat tataaaactg tcaagataca taccaaaaag aaccaataaa
     2761 ggcacataga caaatagtgt caatagtttt aaaacaaaac aagcaacacg acctctagca
     2821 gtaagaggtt tgaataaagc aaagtaagtg acaaacaaac taactaacaa ataagcagta
     2881 atagtactaa gtgtagtaac aggtttaaga ttgtcacgca agacagtaac atcatgaggt
     2941 ggtaatttga gattagccac actcttatta atagtacttt caagagtaag ttgaaatagg
     3001 ccaccaaaag gggatcccat ggatcctggc cttgacggcc cttctgcttt tttgtacaaa
     3061 gttggcatta taaaaaagca ttgctcatca atttgttgca acgaacaggt cactatcagt
     3121 caaaataaaa tcattatttg gggcccgagc ttaagactgg ccgtcgtttt acaacgtcgt
     3181 gactgggaaa acatccatgc tagcgttaac gcgagagtag ggaactgcca ggcatcaaat
     3241 aaaacgaaag gctcagtcgg aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     3301 cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgtga agcaacggcc
     3361 cggagggtgg cgggcaggac gcccgccata aactgccagg catcaaacta agcagaaggc
     3421 catcctgacg gatggccttt ttgcgtttct acaaactctt cctggctagc ggtacgcgta
     3481 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa a
//
