LOCUS       sequence-479313-        3098 bp    DNA     circular SYN 17-AUG-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_HCoV-OC43-Envelope
FEATURES             Location/Qualifiers
     source          1..3098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     710..726
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     710..726
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     731..750
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        731..749
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    767..866
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1175..1274)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1290..1307)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1290..1306)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1299..1321)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      1370..1456
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1548..1575
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1734..1751)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1905..2493)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1985..2004)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2586..3098,1..279))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gtgcacaaca atggtgactt ctacagcgcg gagaatctcg ctctctccag gggaagccga
       61 agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac
      121 cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg ccatccactg cggagccgta
      181 caaatgtacg gccagcaacg tcggttcgag atggcgctcg atgacgccaa ctacctctga
      241 tagttgagtc gatacttcgg cgatcaccgc ttccctcata atgtttaact ttgttttagg
      301 gcgactgccc tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc
      361 gtaacgcgct tgctgcttgg atgcccgagg catagactgt accccaaaaa aacagtcata
      421 acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga
      481 ccagttgcgt gagcgcatac gctacttgca ttacagctta cgaaccgaac aggcttatgt
      541 ccactgggtt cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa ccttgggtag
      601 cagcgaagtc gaggcatttc tgtcctggct ggtctagagg aggcatgatg atatattttt
      661 atcttgtgca atgtaacatc agagattttg agacacgggc cagagctgcc aggaaacagc
      721 tatgaccatg taatacgact cactataggg gatatcagct ggatggcaaa taatgatttt
      781 attttgactg atagtgacct gttcgttgca acaaattgat gagcaattat tttttataat
      841 gccaactttg tacaagaaag ctgggcccat gaggccgcga tcgcgtctta attaagaacg
      901 tcatccacat caaggactgg tggttttaca tcattgtaaa actcataaaa ctgcctacct
      961 ctattaaaca cataaataga aggggacagt actaaggtat tacacatacc gcaaagttga
     1021 atacacaatt taaaagttgc caaaaatgcc actacaacta ttgtaaccaa taaacaaatg
     1081 gcaactataa aaattatttg ccccacatac cacacagtgt ctgcaagata agcatcagcc
     1141 ataaaggatc ccatggatcc tggccttgac ggcccttctg cttttttgta caaagttggc
     1201 attataaaaa agcattgctc atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
     1261 aaaatcatta tttggggccc gagcttaaga ctggccgtcg ttttacaacg tcgtgactgg
     1321 gaaaacatcc atgctagcgt taacgcgaga gtagggaact gccaggcatc aaataaaacg
     1381 aaaggctcag tcggaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1441 cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gtgaagcaac ggcccggagg
     1501 gtggcgggca ggacgcccgc cataaactgc caggcatcaa actaagcaga aggccatcct
     1561 gacggatggc ctttttgcgt ttctacaaac tcttcctggc tagcggtacg cgtattaatt
     1621 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     1681 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     1741 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     1801 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     1861 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     1921 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     1981 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     2041 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     2101 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     2161 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     2221 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     2281 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     2341 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     2401 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     2461 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     2521 tctgacgctc agtggaacga cgcgtaactc acgttaaggg attttggtca tgggtggctc
     2581 gagggttatt tgccgactac cttggtgatc tcgcctttca cgtagtggac aaattcttcc
     2641 aactgatctg cgcgcgaggc caagcgatct tcttcttgtc caagataagc ctgtctagct
     2701 tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca
     2761 tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact
     2821 acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt
     2881 agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc cgctggacct
     2941 accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc aatgtcgatc
     3001 gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc aaattgcagt
     3061 tcgcgcttag ctggataacg ccacggaatg atgtcgtc
//
