LOCUS       sequence-479314-        3536 bp    DNA     circular SYN 25-JUL-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 25, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_HCoV-OC43-Membrane
FEATURES             Location/Qualifiers
     source          1..3536
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             125..916
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1009..1597
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1498..1517
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1751..1768
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1927..1954)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2046..2132)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2181..2203
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2195..2212
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2196..2212
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2228..2327
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3074..3173)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3190..3209)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3191..3209)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3214..3230)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3214..3230)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
       61 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa
      121 cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
      181 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
      241 tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga
      301 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
      361 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
      421 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
      481 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
      541 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
      601 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
      661 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
      721 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
      781 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
      841 ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa
      901 ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg tgagttacgc
      961 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1021 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1081 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1141 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     1201 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     1261 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1321 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     1381 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     1441 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     1501 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     1561 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     1621 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     1681 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     1741 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     1801 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     1861 agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga
     1921 aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat
     1981 ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc
     2041 ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc
     2101 cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt
     2161 aacgctagca tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct
     2221 cgggccccaa ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga
     2281 tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa gcagaagggc cgtcaaggcc
     2341 aggatccatg ggatccagta gtaaaactac accagcacca gtttatatct ggactgctga
     2401 tgaagctatt aaattcctaa aggaatggaa tttttctttg ggtattatac tactttttat
     2461 tacaatcata ttgcaatttg gatatacaag tcgcagtatg tttgtttatg ttattaagat
     2521 gattattttg tggcttatgt ggccccttac tataatctta actattttca attgcgtata
     2581 cgcattgaat aatgtgtatc ttggcctttc tatagttttt accatagtgg ccattattat
     2641 gtggattgtg tattttgtga atagtatcag gttgtttatt agaactggaa gtttttggag
     2701 tttcaaccca gaaacaaaca acttgatgtg tatagatatg aaaggaacaa tgtatgttag
     2761 gccgataatt gaggactatc atactctgac ggtcacaata atacgcggcc atctttacat
     2821 tcaaggtata aaactaggta ctggctattc tttggcagat ttgccagctt atatgactgt
     2881 tgctaaggtt acacacctgt gcacatataa gcgtggtttt cttgacagga taagcgatac
     2941 tagtggtttt gctgtttatg ttaagtccaa agtcggtaat taccgactgc catcaaccca
     3001 aaagggttct ggcatggaca ccgcattgtt gagaaataat atccttaatt aagacgcgat
     3061 cgcggcctca tgggcccagc tttcttgtac aaagttggca ttataaaaaa taattgctca
     3121 tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca
     3181 gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg
     3241 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgcctc
     3301 ctctagacca gccaggacag aaatgcctcg acttcgctgc tacccaaggt tgccgggtga
     3361 cgcacaccgt ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt
     3421 aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc
     3481 agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttgg
//
