LOCUS       sequence-479315-        3686 bp    DNA     circular SYN 23-JUL-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 23, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_HCoV-229E-nsp6
FEATURES             Location/Qualifiers
     source          1..3686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     133..152
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     386..403
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(562..589)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(681..767)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     816..838
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     830..847
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     831..847
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    863..962
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1859..1958)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1975..1994)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1976..1994)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1999..2015)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1999..2015)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2446..3237
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3330..3686,1..232)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
       61 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      121 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      181 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      241 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      301 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
      361 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
      421 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
      481 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta ccgctagcca
      541 ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttagtttga
      601 tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg
      661 ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag
      721 ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg cctggcagtt
      781 ccctactctc gcgttaacgc tagcatggat gttttcccag tcacgacgtt gtaaaacgac
      841 ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag tgacctgttc
      901 gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca aaaaagcaga
      961 agggccgtca aggccaggat ccatgggatc cagtggtaaa accactagta tgtttaaatc
     1021 cataagctta tttgctggct tctttgtcat gttctgggct gaattatttg tttataccac
     1081 cactatttgg gttaaccctg gttttcttac tccgtttatg attttgcttg ttgctttgtc
     1141 actctgtctt acatttgttg ttaaacataa ggttttgttt ttgcaagtgt ttttgttgcc
     1201 ttcaattatt gtggctgcta ttcaaaactg tgcttgggac taccatgtta caaaggtgtt
     1261 ggcagagaag tttgattata atgtttctgt tatgcaaatg gacatccagg gttttgttaa
     1321 catttttatt tgtctttttg ttgcactgtt gcatacttgg cgctttgcta aagagcgttg
     1381 tacacattgg tgcacttatt tgttctcact cattgctgtt ttatacactg cattgtatag
     1441 ttatgactac gttagtttgc tggttatgct actttgtgca atttctaatg aatggtatat
     1501 tggtgctatt atttttagaa tttgtcgttt tggtgttgca tttttaccag tggaatacgt
     1561 gtcttacttt gatggtgtta aaactgtgct gttgttttac atgttgttag gctttgttag
     1621 ctgtatgtac tatggtttgt tgtactggat taacaggttc tgtaagtgca cattaggtgt
     1681 ttatgatttc tgtgttagtc cagccgaatt taagtatatg gttgctaatg gtttgaatgc
     1741 accaaatggc ccttttgatg cgctctttct gtcttttaaa ctaatgggta ttggcggtcc
     1801 tagaaccatt aaagtttcta ctgtacagct taattaagac gcgatcgcgg cctcatgggc
     1861 ccagctttct tgtacaaagt tggcattata aaaaataatt gctcatcaat ttgttgcaac
     1921 gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat
     1981 agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat
     2041 ctctgatgtt acattgcaca agataaaaat atatcatcat gcctcctcta gaccagccag
     2101 gacagaaatg cctcgacttc gctgctaccc aaggttgccg ggtgacgcac accgtggaaa
     2161 cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag
     2221 tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
     2281 gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg
     2341 gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac
     2401 gatgttacgc agcagggcag tcgccctaaa acaaagttaa acattatgag ggaagcggtg
     2461 atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa
     2521 ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac
     2581 agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct
     2641 ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct
     2701 gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc
     2761 gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc
     2821 acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg
     2881 gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg
     2941 ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat
     3001 gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag
     3061 gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt
     3121 gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag
     3181 ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataaccc
     3241 tcgagccacc catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca
     3301 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     3361 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     3421 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     3481 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     3541 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     3601 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     3661 tgcacacagc ccagcttgga gcgaac
//
