LOCUS       sequence-479319-        4866 bp    DNA     circular SYN 24-JUL-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 24, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC7A14_STOP
FEATURES             Location/Qualifiers
     source          1..4866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(33..50)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(204..792)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(284..303)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(939..1748)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1662..1681
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1740..1759
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     1861..1877
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1861..1877
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1882..1901
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1882..1900
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1918..2017
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4340..4439)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4455..4472)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4455..4471)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4464..4486)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      4535..4621
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4713..4740
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
       61 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      121 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      181 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
      241 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      301 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      361 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
      421 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
      481 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
      541 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
      601 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
      661 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
      721 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
      781 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgac
      841 gcgtaactca cgttaaggga ttttggtcat gagcttgcgc cgtcccgtca agtcagcgta
      901 atgctctgcc agtgttacaa ccaattaacc aattctgatt agaaaaactc atcgagcatc
      961 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
     1021 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
     1081 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
     1141 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
     1201 agtttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
     1261 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg
     1321 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
     1381 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
     1441 gtttttccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
     1501 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
     1561 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
     1621 ccatacaagc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
     1681 ccatataaat cagcatccat gttggaattt aatcgcggcc tcgacgtttc ccgttgaata
     1741 tggctcataa caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat
     1801 gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacggg ccagagctgc
     1861 caggaaacag ctatgaccat gtaatacgac tcactatagg ggatatcagc tggatggcaa
     1921 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
     1981 tttcttataa tgccaacttt gtacaagaaa gctgggtctt actcggggct gtaatccagc
     2041 tcgtcattgg cgatcagggc ctcagagttc tgcttgtgct tgctcttaga cttggcctta
     2101 gaggaggtgc ggccattggc cttggcatcg ctcatctgct gatagtaaaa gcccttatcc
     2161 tcggttggtc ctccccagtc ctcctgagac tctccctctg tggcatagct aaagccctcc
     2221 tccacagaga aaggatcgtc cacgtcgtac cgctgatagg tggactggtg cagggcctcc
     2281 tccctggcgc tgatctccag tgtagagttc cagatgccgt agccgaaata gatcagcagg
     2341 cccacaaaac accacacggc gaacctgatc caggtgattg tgctcagctt cagcatcagg
     2401 tagatattca ccagcatggc gaaggcgggc acaaatggca ggcaaggggc catataaggc
     2461 agcttcttgg ggttctctgg ctgctgcagg atcacaaaca ccagggtgga gatcagcagc
     2521 accatcagca ccaccagcag gatggcccac caagactgct cggagatgta atcggagccg
     2581 aagatgataa agctacaaaa gatgaacatc aggatgaaca gcagcagcac gcagatggtc
     2641 actgtgtgtc cggttgctgc tgtaggcctg tccatcttgc ctggcagtcc cagcctgatc
     2701 ctcattgtat agtagtgggg gccgatcagc ttcttcagct tgatcagata gatattctcg
     2761 ctctcatcgg cctcgatgcc ggttgtcatg tccacggtgc cgtagttggg gtgattcacg
     2821 ttatatgtgc tcttatcaga cttgccgatc agcatctcat tgtcgcccag agatggcaga
     2881 ttcttggctc cgcaggtgtt tgttgctggt ccggagaact catcgccctc gctcacgggg
     2941 ctacatgcct ccttctcgca gtcggccagg atgccctcct tcttcttggt gtgctcctcg
     3001 gacaggaact tcacaaagcc atcgatgtcg ctctcgggct ggtagcgcag cagcagcacg
     3061 cacacgctca ccagggtata ggccagcagt gtgccgatgc tcatcatctc gatcagatcc
     3121 ctcagagaca ccagcagggc cagcagggcg gccagaaagc cgctcacgat gcaggccacc
     3181 actggggtct ctgtgtagct agacacgtgg gccagaaatc tgaacagcag tccgtcgcct
     3241 gccattgcgt agatcactct aggcatgggg aacagggatc ccagcaggct cacggtcagt
     3301 cctgccacag atccgattgc caccacaaac ttggcggcgt agaagccgtg ggccacaaac
     3361 atctccatca ggggggactc ggtatcgatt gtatagtatg gcaccatcag ggtcaggatc
     3421 acggacacgc tcacgtaggc tgtcagacag atgaccaggg aggcggtgat ggcatatggg
     3481 atgcttgtat tagggttctt ggcctcctcg ccggttgtgg cgatgatgtc gaagccgata
     3541 aaggcgtaga agcaggttgc tgctccctgc agcacgccgc tccatccgtg tggcaggaac
     3601 tgtccctctg cccaatactt gccattgata aagaacaggc cggcgatcat gataaacacc
     3661 cacacggcca gattcagcac gttcagcaca ttgttgaagc cgatggagtt cttcacgccc
     3721 agggccacga tgatggtcac gatcacggcg atcagcaggg ccagcaggtc agggtagctc
     3781 tcctcgccct tgcccaggcc attcagggtg cccacagaat cggccatcca tctggagatt
     3841 gtgtggttgg ccagagagtc aaacatggag ctcagggcgg atgctcctgc tgctgttccg
     3901 atcagatact ccaggatcag gttccagccg ataaagaagg ccacgaactc gcccacggtc
     3961 acgtaggaat atgtgtaggc gctgccggtt gtcttaggca cccgcacgcc aaactcggcg
     4021 tagcacacgc cagacaggat ggatgccact gctgcgatga tgaaggacac gatcacgcct
     4081 ggtcctgcca tctcctttgc caccagtccg ctcaccacat acatgccggt tcccacgcaa
     4141 gatcccactc ccagggagat cagatccacg gttgtcagca cctgtgccag cttggttccg
     4201 tgtgcggttg tggttcctgt tccctccagc atagactcca caggcttggt tctcaggatc
     4261 cgggagtgca ttgcgtacca tgctgctccc cactgcactc tccttgggtc caggcttgta
     4321 aagaagccag acatggtgga gcctgctttt ttgtacaaag ttggcattat aaaaaagcat
     4381 tgctcatcaa tgtgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgg
     4441 ggcccgagct taagactggc cgtcgtttta caacgtcgtg actgggaaaa catccatgct
     4501 agcgttaacg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgga
     4561 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
     4621 tccgccggga gcggatttga acgttgtgaa gcaacggccc ggagggtggc gggcaggacg
     4681 cccgccataa actgccaggc atcaaactaa gcagaaggcc atcctgacgg atggcctttt
     4741 tgcgtttcta caaactcttc ctggctagcg gtacgcgtat taattgcgtt gcgctcactg
     4801 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     4861 gggaga
//
