LOCUS       sequence-479322-        4341 bp    DNA     circular SYN 06-AUG-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC5A10_STOP
FEATURES             Location/Qualifiers
     source          1..4341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(45..62)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(216..804)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(296..315)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(951..1760)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1674..1693
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1752..1771
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     1873..1889
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1873..1889
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1894..1913
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1894..1912
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1930..2029
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3827..3926)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3942..3959)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3942..3958)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3951..3973)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      4022..4108
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4200..4227
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
       61 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
      121 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
      181 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      241 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      301 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      361 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      421 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      481 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      541 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      601 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
      661 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      721 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      781 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      841 cagtggaacg acgcgtaact cacgttaagg gattttggtc atgagcttgc gccgtcccgt
      901 caagtcagcg taatgctctg ccagtgttac aaccaattaa ccaattctga ttagaaaaac
      961 tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt
     1021 tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca
     1081 agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc
     1141 ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt
     1201 gagaatggca aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc
     1261 tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg
     1321 agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg
     1381 cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat
     1441 acctggaatg ctgtttttcc ggggatcgca gtggtgagta accatgcatc atcaggagta
     1501 cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc
     1561 atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc
     1621 gcatcgggct tcccatacaa gcgatagatt gtcgcacctg attgcccgac attatcgcga
     1681 gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg cctcgacgtt
     1741 tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt
     1801 attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacacg
     1861 ggccagagct gccaggaaac agctatgacc atgtaatacg actcactata ggggatatca
     1921 gctggatggc aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt
     1981 gataagcaat gctttcttat aatgccaact ttgtacaaga aagctgggtc ttaggcgaaa
     2041 taggcgtaaa agaagatatt cacgcacatc agcaggatgg cgttaaagcc gcacacccgt
     2101 gcccagaatg cgtgcttctg tggtgtctgt ccatcgcctg ccttggttcc cagaggcacg
     2161 tcctgtgcca gtgtccacca ggtcagattc tcgatctgca cagactgggg tggaggggtc
     2221 agcagggatc ctgccaccac cactgctccg ctcagtgcaa acagggccac tgcgaagtgc
     2281 agatagtgga tggatcccag cacggctggc ctggtatcag gctctccgca tggtggtgct
     2341 ggattcagaa actccagcac cagccttgtt gctcccacca ccagtcctgc gatcagtccc
     2401 cagaatgctc cctgctcgtt tgccctcctc caaaacacgc ccagcacgaa cacggctgtc
     2461 acaggtggtg ccaggctaga ggtcacgctc tgcatgtaga taaacagctg gccgctgtta
     2521 gaatcctgca gcacggggat ccatgccacg ctcacgccga tcagggccac gatgaccagc
     2581 cggcccacca gcagcagctc cctctctcca gagcgtggcc tcagtctccg ccagatgtcc
     2641 attgtaaaca gggtggagct agaattgaag atggaggtca gggagctcat cagggcggcc
     2701 agcatcacgg cgatcatcag gccgcgcagg ccgataggca tcagctccat gaccagcttg
     2761 gggtaggcga tgttggaaca gcccacttct gctccacatg ctctcaggca ctcgcttggc
     2821 accacgcagc ccacatcgtc agggaacagg gcccggctga tcattcctgg catgatgatc
     2881 aggcccattg gcagcatctt cagataggat gccaggatgc ttcctgcctt tgcgtgattc
     2941 agatcgcggg cggacaggct cctctgcacg atgacctggt cggtacacca gtaccatgtg
     3001 gccatgatgg tcaggccaaa tgtcatgccg gtccaaggca gatcgccggt gtgtgggtcc
     3061 ctgaacatgt gcatggcatc tgtcctaggc aggtggcatg tggtgtttgc gatggtcctg
     3121 gatgggattg cctgtgcata tgctgcctcc agctgtccgt atcctccgat ctgatcaaag
     3181 gccttgatgg tcaggatcac ggcgcccacc accatgatca gtgtctgcag ggcgtcggta
     3241 tagatcacgg ctgccagtcc tcctgcgatt gtgtacaggg cggtgatgcc cagtgtcagg
     3301 atggtagaca ggtagaagtt ccagcccaga cagatgtgca cgaacagggc gcctgcgtac
     3361 aggtccaggc tgatcttggt gaacacgctc agcagcagag acagcacgga caggtacatt
     3421 ctgatccgct ggccgccata tctcttctgg atgtactcgg gcagggtcac gatctcagag
     3481 gagatataga ttggcacaaa cacccaggcc agggccagca gcacgtatgt tgcgttccac
     3541 tcgaatcctg ccactgccag gcctcctgct gctccgcttc ctgccagtcc gataaacagg
     3601 ccagagccct cgctagatgc gaacagggat gctccgattg gccaccaggt catgtccctt
     3661 cctgccagaa aatagccatt cactgtgttt ctagaggccc tgcaggagct ccagattccc
     3721 actgccacat tcagggcgaa gtacacggtg atcacgatga tatcggccac ggacagctgt
     3781 gtgccagggg tgtgcaggtc agatgtggag ttggcggcca tggtggagcc tgcttttttg
     3841 tacaaagttg gcattataaa aaagcattgc tcatcaatgt gttgcaacga acaggtcact
     3901 atcagtcaaa ataaaatcat tatttggggc ccgagcttaa gactggccgt cgttttacaa
     3961 cgtcgtgact gggaaaacat ccatgctagc gttaacgcga gagtagggaa ctgccaggca
     4021 tcaaataaaa cgaaaggctc agtcggaaga ctgggccttt cgttttatct gttgtttgtc
     4081 ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgtgaagca
     4141 acggcccgga gggtggcggg caggacgccc gccataaact gccaggcatc aaactaagca
     4201 gaaggccatc ctgacggatg gcctttttgc gtttctacaa actcttcctg gctagcggta
     4261 cgcgtattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     4321 ctgcattaat gaatcggcca a
//
