LOCUS       sequence-479323-        4473 bp    DNA     circular SYN 28-JUL-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 28, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLCO1B7_STOP
FEATURES             Location/Qualifiers
     source          1..4473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(85..184)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(200..217)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(200..216)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(209..231)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      280..366
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      458..485
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(644..661)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(815..1403)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(895..914)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1550..2359)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2273..2292
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2351..2370
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     2472..2488
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2472..2488
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2493..2512
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2493..2511
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2529..2628
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 caaaagagcc gtcgatcagg cccaccagag aggagctgat ctcgaatctc cgctcgatct
       61 gtgtggtgga gatcttcatg gtggagcctg cttttttgta caaagttggc attataaaaa
      121 agcattgctc atcaatgtgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
      181 tttggggccc gagcttaaga ctggccgtcg ttttacaacg tcgtgactgg gaaaacatcc
      241 atgctagcgt taacgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag
      301 tcggaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg
      361 acaaatccgc cgggagcgga tttgaacgtt gtgaagcaac ggcccggagg gtggcgggca
      421 ggacgcccgc cataaactgc caggcatcaa actaagcaga aggccatcct gacggatggc
      481 ctttttgcgt ttctacaaac tcttcctggc tagcggtacg cgtattaatt gcgttgcgct
      541 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
      601 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
      661 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
      721 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
      781 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      841 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      901 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      961 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1021 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1081 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     1141 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     1201 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     1261 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     1321 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     1381 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     1441 agtggaacga cgcgtaactc acgttaaggg attttggtca tgagcttgcg ccgtcccgtc
     1501 aagtcagcgt aatgctctgc cagtgttaca accaattaac caattctgat tagaaaaact
     1561 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
     1621 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
     1681 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
     1741 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
     1801 agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct
     1861 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
     1921 gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
     1981 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
     2041 cctggaatgc tgtttttccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
     2101 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
     2161 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
     2221 catcgggctt cccatacaag cgatagattg tcgcacctga ttgcccgaca ttatcgcgag
     2281 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctcgacgttt
     2341 cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta
     2401 ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacgg
     2461 gccagagctg ccaggaaaca gctatgacca tgtaatacga ctcactatag gggatatcag
     2521 ctggatggca aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg
     2581 ataagcaatg ctttcttata atgccaactt tgtacaagaa agctgggtct tactgctcct
     2641 cggcgcttgg cacaaagtgc tcagaatcat tcaggaactc caggttggcc tcgtccatga
     2701 cctggcgctc gttctccagc acctttgtat ccttgccgtg ggacttcttc ctcaccacga
     2761 agataaacac ggtcagcagc accagcacag gaaagatcag ggccaccttc aggccgaaaa
     2821 aggcccggcc cagatatgtg ctattgtaga ttctacaggc tccccttgct ccgcaggagt
     2881 tggtgctcca cttcatacat gtggtatcga tcagggcgcc aaaatagatt ggcaccagga
     2941 tgccgcccag agatctcatg atcatggagt ggaagccgat ggccagggcc ttcagctcag
     3001 gctgcacgat ccggatgacc agcacgctgt aagatgtcag gcccacggcg cacagaaagg
     3061 cgtccagcac ctggatcacg aaatacacgt agctcttgcg ggtacatgca tcgtcccttg
     3121 ggcactctcc caggtgggcg gaataattct tgttctgcag gccgatcact tccacgcagc
     3181 tgcaattgta gaacacgatt ggctccttgt tgccagagga gctcttacag ccggccaggc
     3241 aagggctcag gtaggtgatg ccattgttgc cgcacacggg ctcccactga gactcgtcac
     3301 aattgcactc gctgttgcaa taagacagtg gcacatccac gtgggaccgc actgggctat
     3361 tgccgtcgta tgtcagggtc aggccggcca cggacttgct ctcacagatc aggaaaaagt
     3421 acagcacctg agacagcagg tgcacggtgg cggagcaaaa ggccagcttg gccaggccca
     3481 ccagagacag cttaaacttc ttgatgatat atcctccgga gaacattccg attgccactg
     3541 caggcagggc cagcacgccc agcaggaagt ttgtcttaga ggcggaccag ccgtactgct
     3601 gctccaccat cttgatgata taggtcaggg aggcgatgta agaggacatg tgcagcagtg
     3661 tgaagatcac aaagatcaca tacaggggat tggtcaggat agacttcagg gactggaaaa
     3721 agcctgtcac gttcttggtg atgtacttgc gcctatttgt caggttggcg atctgatttc
     3781 tcttatcgtt ggtcttcagc acgtgcagga acaggctcac cttccgctcc ttctgaggct
     3841 tattggggtt cagtggcagg aaaaagaaag ggatgctaga gatgatagac acgatgccgg
     3901 acaccagaaa gcccagccac catgctccca cccatctgct gtccttgggt gtgatccgga
     3961 tggtagacag atccacatag ccgatgtcca cgtacatctt ggcgaacagg gagcccagca
     4021 tgaaggcaaa caccaggcct gtcatgccca tcacattcac ggtgcccaga tacagggagc
     4081 tgtggccctc cttggcaaaa tcgtcgatgt agctgatgcc cagaggcacg atgggtgtct
     4141 cgccgatgcc tctcagcatg ttgcccatga acacgtagat ccacatgtgg ctgccagact
     4201 ccttcacgca tcccctctcg atgatctcgg atggggtcct attcaggctc agcatctggt
     4261 tgatcaggca atttggcagg ttggatgtgc tattctcgga aggatcgatg ttggtctcct
     4321 tgctatagcg atagtagccc ataaagaagt ggggcagtgc catcaggatg cttccggttc
     4381 ccatcaggaa gcagccgatg ccgatcagct ttggcctgtg cagcttgctg ccaaagtaag
     4441 acacgaacac gatcacaaac aggttgccga tct
//
