LOCUS       sequence-479333-        4089 bp    DNA     circular SYN 19-AUG-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 19, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC2A7_STOP
FEATURES             Location/Qualifiers
     source          1..4089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(48..636)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(128..147)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(783..1592)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1506..1525
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1584..1603
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     1705..1721
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1705..1721
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1726..1745
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1726..1744
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1762..1861
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3407..3506)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3522..3539)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3522..3538)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3531..3553)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      3602..3688
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3780..3807
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(3966..3983)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
       61 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
      121 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
      181 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
      241 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
      301 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
      361 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
      421 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
      481 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
      541 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
      601 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
      661 gggtctgacg ctcagtggaa cgacgcgtaa ctcacgttaa gggattttgg tcatgagctt
      721 gcgccgtccc gtcaagtcag cgtaatgctc tgccagtgtt acaaccaatt aaccaattct
      781 gattagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca
      841 ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc
      901 cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa
      961 cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg
     1021 actgaatccg gtgagaatgg caaaagttta tgcatttctt tccagacttg ttcaacaggc
     1081 cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat
     1141 tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc
     1201 gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga
     1261 tattcttcta atacctggaa tgctgttttt ccggggatcg cagtggtgag taaccatgca
     1321 tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag
     1381 tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga
     1441 aacaactctg gcgcatcggg cttcccatac aagcgataga ttgtcgcacc tgattgcccg
     1501 acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc
     1561 ggcctcgacg tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa
     1621 gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta acatcagaga
     1681 ttttgagaca cgggccagag ctgccaggaa acagctatga ccatgtaata cgactcacta
     1741 taggggatat cagctggatg gcaaataatg attttatttt gactgatagt gacctgttcg
     1801 ttgcaacaaa ttgataagca atgctttctt ataatgccaa ctttgtacaa gaaagctggg
     1861 tcttaaaaag aggtctcctt ggctggggat gctgtaggtg gtcctgcatc gatggtctcc
     1921 tccttctcct ctggcagctt cacgcgattc ctcttggcaa agattctgtt gatctccacg
     1981 aatgtcttgc ccttggtctc tgggatgacc acatagatgt agatggcggt cagcagacag
     2041 atgccggcaa agatgatgaa ggaataggcg ccgatggcct cctggatgct aggaaacagg
     2101 aagccgatga tgaagttggt cagccagtgc acggcgccgt ccaccataaa ggcggctctc
     2161 cgggagctct gcaggaagat ctctgtccgc accacggaag gcactgggct tggtccgata
     2221 gagtgtcctg cgatataggc aaacacgcag atgatgccca ggtagctcag ctcaggcacg
     2281 cgattctgga acagcagcac cacggtcagc accagacagg cagatccgca gattccgtat
     2341 cctgccagca gcaggtggcg cctgcccagc ctctccacca gcacggcgct tgtgatggtc
     2401 atcacgatgt tcaccacgcc gctgcccact gtcacatact gagagtgtgc tgcctccact
     2461 ccggcggatg tgtagatggt atcggcatag taattgatgg cgttgatgcc gctcagctgc
     2521 tgtcctgcca tcagcacgat gatggacagc agctgccatc tcaggctccg cagtgcgcac
     2581 aggtgcagca cggacaggtg tccctctgcc ctctctgccc ttgcctctgc tctcatatcc
     2641 tccagctctg cctccatgtc ggtgtgtccc ctcagtctcc gcagggcctg ccttgctgtg
     2701 gcctcgtcgc ccttctggat cagggagtac cgagggctct cgggaaagaa tggcagggtc
     2761 agcagctgca gcagggcggg cacgcctgtc agggccagca gcacgggcca tcctgctgga
     2821 tttcccagga ttgcctgcag gctaaagatc tgggccagga acacgcccac gatcacgaac
     2881 acctctgtca tggtgcccac catgcctctc aggttcttag gggccagctc gcccagatac
     2941 atgggcaggg cgctgtaaga gattcctgcg cacacgccca gcaccaccct agaaaataca
     3001 ataagttcga aggctttcgc caccttggac acgcccatca ggatggcagg gatgatggca
     3061 aagatattgt tgatcagcag ggtgcccttg cggccacaag aatccaccag caggcccacc
     3121 agcagggatc ccagcagtcc tcccagtggg aacatagaca ctgtgcagga ccacagcagc
     3181 agcatcagct tgccgtccat gaaggtggcg tgcctctcga aataggtctc attgtaaaag
     3241 ctcttgaaca ccttgtgtgg ggtattcacc acagacaggt tatagccgta ctgaaaggcg
     3301 ctgccgaagg cggcagacag tgtggccagc agcagggttg gctgcagtct gccctccctg
     3361 gaagggattg gaggtggtgt tcctgcctcc ttgttctcca tggtggagcc tgcttttttg
     3421 tacaaagttg gcattataaa aaagcattgc tcatcaatgt gttgcaacga acaggtcact
     3481 atcagtcaaa ataaaatcat tatttggggc ccgagcttaa gactggccgt cgttttacaa
     3541 cgtcgtgact gggaaaacat ccatgctagc gttaacgcga gagtagggaa ctgccaggca
     3601 tcaaataaaa cgaaaggctc agtcggaaga ctgggccttt cgttttatct gttgtttgtc
     3661 ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgtgaagca
     3721 acggcccgga gggtggcggg caggacgccc gccataaact gccaggcatc aaactaagca
     3781 gaaggccatc ctgacggatg gcctttttgc gtttctacaa actcttcctg gctagcggta
     3841 cgcgtattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     3901 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     3961 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     4021 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     4081 tgagcaaaa
//
